We narrowed to 792 results for: Kin
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TypeBlog PostPublishedAug. 20, 2015, 2:30 p.m....and develop an area of expertise. Whether you’re seeking a full-time job or want to be an independent grant...a mentor, find your people Find someone who is working or has worked as a Grant Professional to mentor...
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Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog PostPublishedMay 15, 2014, 3:33 p.m....optogeneic actuators and sensors at Addgene. Cell marking/selection: Expression constructs like plasmids ...Because of its stability, GFP can be used in lineage tracking capacities in cell fate studies. It can also be... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog PostPublishedApril 23, 2014, 1:08 p.m....many questions from scientists all over the world looking for some additional information or clarification...functional before investing time and resources into making a stable cell line. When expressing a viral construct... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog PostPublishedFeb. 17, 2022, 2 p.m....tools for cellular targeting. Lately, they’ve been working on two tools that expand and exploit CRISPR, both...Jonathan and experts in CRISPR evolution, were also working on the project. Using computational observations... -
Antibodies 101: The Four ELISAs and When to Use Them
TypeBlog PostPublishedJuly 25, 2024, 1:15 p.m....The assay only uses one antigen binding antibody, making it less specific than other methods. In addition...positive control will confirm that the procedure is working and should always be included. For a negative... -
Hot Plasmids Spring 2024
TypeBlog PostPublishedMay 23, 2024, 1:15 p.m....high fidelity during action potentials of fast-spiking neurons. The same A-to-D mutation in the related...useful materials for research into Alzheimer’s, Parkinson’s, ALS, and Huntington’s Disease, including plasmids... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog PostPublishedJuly 23, 2019, 12:46 p.m....independently identified LY6A as a receptor for PHP.B by looking for a unique sequence of DNA that was present in...galactose and the AAV receptor (AAVR). Cell lines lacking galactose were still transduced by PHP.B but only... -
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog PostPublishedMay 2, 2019, 12:41 p.m....clear plans to connect Genotype to Phenotype thus making progress on the NSF “G to P grand challenge.” Creating...like people. This is actually rare in the animal kingdom. Gasterosteus aculeates (Dan Bolnick) A paper on... -
Designing Your Chalk Talk for the Academic Job Interview
TypeBlog PostPublishedMarch 12, 2019, 1:37 p.m....research vision uninterrupted and then you will begin working through your aims over the next 45 minutes. The...salient details. Make the most of your limited speaking time. Beyond the information you seek to convey... -
Hot Plasmids - August 2020
TypeBlog PostPublishedAug. 18, 2020, 1:15 p.m....the yeast extracellular surface) which enables tracking of protease activity with a fluorescent protein...that the best motif was from the Lck gene. For marking microtubules of mammalian cells, the author found... -
Finding nucleic acids with SHERLOCK and DETECTR
TypeBlog PostPublishedApril 16, 2020, 1 p.m.... Specific High Sensitivity Enzymatic Reporter UnLOCKING (SHERLOCK and SHERLOCKv2) (Gootenberg et al., ... Specific High Sensitivity Enzymatic Reporter UnLOCKING Cas13 (C2c2) was first identified in 2016 by the... -
Mesothelioma - Causes, Symptoms, and Treatment
TypeBlog PostPublishedDec. 13, 2016, 3:30 p.m.... used as a test to see how well treatments are working to fight the cancer. Beyond early modifications...Asbestos Disease Awareness Organization (ADAO) are working toward such a ban, and the president of the organization... -
Getting the Most from Your Lentiviral Transduction
TypeBlog PostPublishedApril 7, 2016, 2:30 p.m....lentivirus to be time consuming, difficult, and lacking in reproducibility. Read on for some tips for getting...overly confluent or too old. Ensuring that you are working with healthy, happy cells is one of the easiest... -
Plasmids 101: E. coli Strains for Protein Expression
TypeBlog PostPublishedFeb. 10, 2015, 3:06 p.m....positive selection and a p15A origin of replication, making it incompatible with other p15A plasmids. pLys ...of Replication The Promoter Region – Let's Go! Looking for More Plasmids 101 Articles? Find them all ... -
Tips for Using FRET in Your Experiments
TypeBlog PostPublishedNov. 5, 2014, 3:41 p.m....biologist you would say. The student was of course talking about the now well-known FRET, aka Fluorescence...and the extinction coefficient of the acceptor (linking the quantity of absorbed light, at a given wavelength... -
Antibodies 101: Single Chain Fragment Variables (scFvs)
TypeBlog PostPublishedJune 3, 2021, 1:15 p.m.... signal is boosted and enables single molecule tracking in living cells. The HA frankenbody is an HA tag...also be fused to cellular toxins, radioisotopes, cytokines, and enzymes for cancer, autoimmune, and/or inflammatory... -
Antibodies 101: Normalization and Loading Controls for Western Blots
TypeBlog PostPublishedApril 8, 2025, 1:15 p.m.... are equal. Created with BioRender.com. This kind of normalization is a nearly ubiquitous tool, but...controls So how can you address these errors without making incorrect assumptions? The answer depends on the... -
Plasmids 101: Biotinylation
TypeBlog PostPublishedNov. 15, 2018, 1:50 p.m....fed a diet of primarily egg whites would develop skin lesions and other terrible health issues. This disease...PMC3223083. 6. McInnes, J. L., Forster, A. C., Skingle, D. C., & Symons, R. H. “Preparation and uses of... -
SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas
TypeBlog PostPublishedMay 5, 2020, 1:15 p.m.... so, the WHO recommends one strategy: testing, tracking, and social distancing. With countries like South...relies on its activity on the target regions thus making it an affinity-based method. Due to the mismatch... -
Tips for Getting a Faculty Position
TypeBlog PostPublishedJuly 20, 2023, 1:15 p.m....thoughts on research statements. Snapp, who is always looking for ways to improve his eBook, adds: "If you have... fit into a department and bring in grants. What kind of colleague will you be? Do you understand what...