...recombination at a frequency three orders of magnitude higher than identical targeting vectors delivered through...Other investigators who had been working to develop high efficiency gene targeting approaches improved on...repair, it has proven more challenging to develop high efficiency CRISPR-based approaches for the generation...

.... This over-reliance on the perfect story for a “high impact” publication can lead to the publication ...reproducibility issues in the biological sciences. I’d highly recommend listening to the panel discussion to ...Scientific Figures into Dashboards and Share Your Insights Who gives a tweet? 9 facts about scientists on...

...important that your gRNA sequence is designed to be highly active and reduce off targets. For CRISPRa and ...consuming process and should not be undertaken lightly! Indeed, truly achieving the gold standard requires...genetic screens using genome-wide libraries have shown high concordance between different sequences targeting...

... LITE system, which uses light to regulate transcription, also includes light-regulated histone methyltransferases... CA (2017) CRISPR–Cas9 epigenome editing enables high-throughput screening for functional regulatory elements...

...may be a good choice for targeting neurons, with high transduction frequencies in the central nervous ... our in-stock viral prep service.  AAV8 AAV8 has high transduction efficiency in multiple tissues, rapid...AAV9 AAV9 has relatively broad tissue tropism and high transduction efficiency, and is an excellent choice...

...using them. Indeed, the future of mouse work is bright as it is now easier than ever to manipulate the...technology, CRISPR, that is simple in concept, straightforward to use, and robust in performance. In its natural...BL, Xavier RJ, Root DE (2014) Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation...

.... PubMed PMID: 18845712. 9. Warren, L., et al., Highly efficient reprogramming to pluripotency and directed...PubMed PMID: 26189426. 15. Si-Tayeb, K., et al., Highly efficient generation of human hepatocyte-like cells...Central PMCID: PMC2946078. 16. Zhang, D., et al., Highly efficient differentiation of human ES cells and...

...existing content. Access the CRISPR Guide here! Highlights include a much-anticipated prime editing section...

... an antibody that works, we highly recommend “Chapter 3: Finding the Right Antibody for Your Experiment...

...protein for controlling molecular processes with light. Listen to Our Segement on PhoCl Segment  Hot Plasmid...Plasmid 4: pPSU Plasmids for generating DNA molecular weight markers.  Listen to the Segement on the pPSU Plasmids...

...generating knockouts using CRISPR and Wednesday will highlight some Cas9 variants. Next, we’ll look at various...

...resources! Ready to sing along with this year's lineup? Right! We're ready to, so let's get started!  On the...sequences🎵On the 2nd day of CRISPR, Addgene gave to me: High-fidelity Cas13 variants for targeted RNA degradation...

...from the first generation of base editors to the eighth generation (and dual base editors, too!).  On the...gave to me: CRISPR methods for bacteria.  On the eighth day of CRISPR, Addgene gave to me: anti-CRISPR ...

...adorned with a bioluminescent tree topper, tube “lights,” and autoclave tape garland. Thanks @ribosholmes... bit of everything: frog snowflakes, ornaments, lights, and took a group photo wearing holiday sweaters...

... In each short episode of this series, we’ll highlight one tip that we’ve learned in the lab that will...

...to make inexpensive 100 bp or 1 kb DNA molecular weight ladders were recently deposited with Addgene. A... The pPSU Plasmids for Generating DNA Molecular Weight Markers. Scientific Reports,7(1). doi:10.1038/s41598...

... world making breakthrough discoveries!” I knew right then I had found my dream job, because one thing...latest technologies and breakthrough research. Insights about how to navigate through all stages of your...

...rap mastermind, Tom McFadden, recently worked with high school students in the bay area to create a plasmid...rap video for us (If you’re new to plasmids, we highly recommend checking out the video). Tom has made...

...every new lab mate? Do you have a trick to get the highest yield out of your minipreps? If so, we’d love to...Addgene Blog Possible guest topics Some topics you might consider are: An overview of software tools useful...

...each of the Hot Plasmids segments into its own (highly Tweetable!) audio clip. In the future, you’ll be...Domain Constructs Kit from John Chodera's Lab - Get high expression of His-tagged human kinases in bactera...