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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 817 results for: nin

Showing: 601 - 620 of 817 results

  1. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ...command line, and so was really starting from the beginning. Luckily, a fellow graduate student and a data... entered is useful for beginners who are still learning the basics. However, these notes take up a lot...

  2. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ... for publication and is, in most cases, blind (meaning you don’t know who the reviewers are). If the reviewers...future. Disregard reviewer’s comments without explaining why. If it looks like you have ignored their ...

  4. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...interactions, Ramanathan et al. designed an RNA bait containing three well conserved but not well characterized... and post-transcriptional regulation of SM1v1-containing RNAs could play a role in the severity of breast...

  5. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ... additionally provides detailed information on cloning procedures used to construct the deposited plasmids...Considerations when getting the dTAG system up and running in your lab Confirm the functionality of your fusion...

  6. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    Published
    Oct. 1, 2019, 1:21 p.m.
    ...Do I have to be on LinkedIn?” For anyone who is planning or might need to plan for a career outside academia...hiring managers will think it is odd. For those planning a career in academia, it may not be required, ...

  7. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Published
    July 25, 2017, 1:20 p.m.
    ... two dimerizing photoswitchable Dronpa (dron= a ninja term for vanishing, and pa= photoactivation) domains... psMEK2, psRaf1, and psCDK5. MEK2 is a serine/threonine kinase with a similar 3D structure to MEK1, but...

  8. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ... used in research have their genomes “gutted”, meaning that all viral genes except ITRs (inverted terminal...capacity Empty rAAV has a cargo capacity of ~ 4.7 kb, meaning that your gene of interest (or genes) need to be...

  9. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ... of solute channels that are responsible for maintaining the nutrient and ionic balance of the cells,”...the skin making it important to study what is happening within the keratinocytes. “We hope to use CRISPR...

  10. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...target opposite DNA strands and specify a large intervening deletion. Not only can this method precisely ...induces two double-strand breaks and deletes the intervening sequence. As with other prime editing tools, ...

  12. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...coregulator-related global gene profiling datasets in to our data mining tool, Transcriptomine. We are currently nearing...2001). Nuclear receptors and lipid physiology: opening the X-files. Science 294, 1866-1870. (PubMed) Lonard...

  13. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...ncRNA locus (Ho et al. 2012). For large-scale screening, sgRNA libraries are useful for targeting thousands...092902 Liu Y, Cao Z, Wang Y, et al. Genome-wide screening for functional long noncoding RNAs in human cells...

  14. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ...with more stringent stripping methods. If you are planning to detect multiple proteins through several rounds...membrane once before any detection steps to avoid retaining residual antibodies. If you have already applied...

  15. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...architecture and plumbing are important. We started with learning the head is connected to the neck. Shortly after...M, Ghanem A, Conzelmann KK, Lepier A, Götz M, Berninger B. Retrograde monosynaptic tracing reveals the...

  17. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ...anti-CRISPRs for a type II system in Neisseria meningitidis (Nme) (Pawluk et al., 2016). While Streptococcus...most used and well studied CRISPR system, N. meningitidis can also be used for genome editing. In this...

  18. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...Several variants are now circulating and plasmids containing these mutations have been deposited at Addgene...more about it on the blog. COVID-19 research is happening fast and we’re glad to join these efforts and ...

  19. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ... about a week. Taking advantage of Golden Gate cloning, the Haynes lab’s Protocols.io protocol describes...useful vectors compatible with Gibson and Saptrap cloning systems to generate AID* tagged CRISPR knock-in...

  20. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...spread out the risk for the project by initially designing and ordering several dozen versions of the toehold...genome as unique barcodes to identify samples containing the virus. Of the 48 sensors, targeting 24 regions...
Showing: 601 - 620 of 817 results