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TypeBlog PostPublishedFeb. 5, 2019, 1:04 p.m....page Read our transferable skills guide Read blog posts about career readiness Resources at Addgene.org... their lab, but for many graduate students and postdocs, the people you see in your bay for 8-12 hours...floundered for a solid year after I started my postdoc! It’s a lot easier to manage your time. As my...
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Bioinformatics at Addgene
TypeBlog PostPublishedJuly 14, 2022, 1:15 p.m....pipeline, we needed a user interface. In our next post, we’ll share our story about how we used Nextflow... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog PostPublishedSept. 28, 2017, 2:01 p.m....expression in mammals. Subscribe to Viral Vector Blog Posts Additional Resources on Addgene.org Search for... -
Plasmids 101: Shuttle Vectors
TypeBlog PostPublishedMay 20, 2025, 1:15 p.m....a distinct gene delivery method. We focused this post on shuttle vectors delivered by transformation and... -
Hot Plasmids - November 2023
TypeBlog PostPublishedNov. 14, 2023, 3:35 a.m.... Hot Plasmids! Here's what you'll find in this post: New compact, efficient prime editors A versatile... -
Hot Plasmids: Winter 2025
TypeBlog PostPublishedMarch 11, 2025, 1:15 p.m....Plasmids articles. Here's what you'll find in this post: Optimized prime editing in dicots Self-labeling...subthreshold events, including miniature excitatory postsynaptic potentials (mEPSPs) (Hao and Lee et al., 2024...Anti-APP [6E10-A5] recombinant antibody in human postmortem brain samples. Results show positive staining... -
Design Tips for Prime Editing
TypeBlog PostPublishedJan. 23, 2025, 2:15 p.m....We recently updated our blog post on Prime Editing, and that meant rereading many of the original papers... -
Magnetic Control of Proteins: More than a Dream
TypeBlog PostPublishedSept. 24, 2024, 1:15 p.m....-publishing directly on his website (and often posting updates on social media) and freely sharing code... -
CRISPR 101: Making a Knock-In Cell Line
TypeBlog PostPublishedDec. 22, 2022, 2:15 p.m....as part of the templated DNA. Check out our blog post on increasing HDR efficiency to read more about ... -
Career Insights: Technical Support Specialist
TypeBlog PostPublishedNov. 9, 2017, 2 p.m....Perkins and Stephanie Hays for images used in this post. Klaus Wanisch is an Outreach Scientist at Addgene...only become obvious at rather late stages (i.e. postdoc level) and can include the pressure to publish ...become more appealing on the job market (e.g. a postgraduate degree in law, an MBA, or similar), there are...developed by life science PhDs. In my days as junior postdoc I was aware that such roles existed, but did not... -
Fluorescent Proteins 101: Fluorescent Protein Timers
TypeBlog PostPublishedMay 4, 2017, 2:30 p.m....consequences of FP aggregation, as Erik Snapp’s blog post shows. To avoid these issues, Subach et al. and ... -
How to Write a Scientific Review Article
TypeBlog PostPublishedFeb. 16, 2017, 3:30 p.m....currently stuck trying to write one, hopefully this post will help you get things moving - remember you're... -
Plasmids 101: Gateway Cloning
TypeBlog PostPublishedJan. 12, 2017, 3:30 p.m....recombined construct. Read our recent Plasmids 101 Post on CcdB for more information. How to clone using... -
The Challenges of Cell Culture
TypeBlog PostPublishedMay 5, 2016, 2:30 p.m....This post was contributed by ATCC Outreach Scientist Nick Amiss. Cell culture in the present day Cell... -
CRISPR Antimicrobials
TypeBlog PostPublishedMay 3, 2016, 2:30 p.m....antibiotics are easy to see. As discussed in this blog post, most bacteria have poor non-homologous end joining... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog PostPublishedOct. 6, 2015, 2:30 p.m....Avaliable on the Addgene Blog Read more Inside Addgene Posts! Learn all about Plasmids in our Plasmids 101 series... -
Light Sheet Fluorescence Microscopy
TypeBlog PostPublishedJuly 16, 2015, 2:30 p.m....This post was contributed by Jae Lee and Pantelis Tsoulfas of the Department of Neurological Surgery ...the direction of the specimen, A= anterior, P= posterior, D= dorsal, V= ventral Developments in light sheet... -
Plasmids 101: Control Plasmids
TypeBlog PostPublishedApril 9, 2015, 3:29 p.m....Region – Let's Go! Browse All of Our Plasmids 101 Posts ... -
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog PostPublishedDec. 2, 2014, 7:23 p.m.... the future. Addgene: What advice do you give to post-docs and graduate students as they complete their... -
Filming Science Videos in the Age of Social Distancing
TypeBlog PostPublishedMay 20, 2021, 1:15 p.m....She rolled several long takes, and we cut it up in post. Test: Overall, it worked. We pulled it off! Check...