We narrowed to 694 results for: ust

1. Giving gRNAs a Facelift - Synthetic and Beyond

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   Blog Post

   Published

   Oct. 20, 2022, 1:15 p.m.

   ...O-methyl modifications, proved more stability than just one modification alone (Hendel, et al., Nat Biotechnol...

2. Antibodies 101: Selecting the Right Antibody

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   Blog Post

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   March 15, 2022, 1:15 p.m.

   ...by price point, shipping time, or other factors. Just remember that these are secondary considerations...

3. Antibodies 101: Polyclonal Antibodies

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   Blog Post

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   July 14, 2021, 1:15 p.m.

   ...production of monoclonal antibodies starts with selecting just one of these B cell clones for further antibody ...

4. RNA Extraction Without A Kit

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   Blog Post

   Published

   March 24, 2020, 1:15 p.m.

   ...protein denaturant, guanidinium thiocyanate, did just this (Chirgwin et al., 1979). They developed a protocol...

5. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

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   Blog Post

   Published

   April 13, 2017, 2:30 p.m.

   ...color-emission biosensor named after the brightly-coloured Australian parrot. It’s comprised of two tandem fluorescent...

6. CRISPRainbow and Genome Visualization

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   Blog Post

   Published

   Feb. 28, 2017, 3:30 p.m.

   ...different Cas9 ortholog, and the target sequence must be located adjacent to that PAM. To scale up fluorescent...

7. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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   Blog Post

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   March 19, 2019, 1:31 p.m.

   ...interactions in cells because the protein binders can be customized for protein visualization, relocation, degradation...

8. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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   Blog Post

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   Jan. 31, 2019, 1:32 p.m.

   ...development, the microenvironment backstage play just as important of a role if not more.  Many thanks...

9. Plasmid Tools for Microbiome Studies

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   Blog Post

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   June 27, 2019, 12:25 p.m.

   ...Jeffrey Barrick’s labs at The University of Texas at Austin recently developed the Bee Microbiome Toolkit (...

10. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type

    Blog Post

    Published

    Nov. 3, 2020, 2:15 p.m.

    ...localization, kinetics, and spectral properties, must be optimized simultaneously in order to generate...

11. New Tool for Lineage Tracing: The ClonTracer Library

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    Blog Post

    Published

    Sept. 22, 2015, 2:30 p.m.

    ...Novartis because she wanted first-hand experience in industry research. When she came to Novartis, she was happily...

12. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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    Blog Post

    Published

    April 22, 2015, 2:06 p.m.

    ...also proteins, like antibodies. But we are really just using what biology has given us. Wood is a complicated...

13. Plasmids 101: Cre-lox

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    Blog Post

    Published

    Jan. 13, 2015, 3:47 p.m.

    ...protein tags, and antibiotic resistance markers (just to name a few). In this edition, we’re going to ...

14. A Look at Addgene's QC Process

    Type

    Blog Post

    Published

    May 6, 2025, 1:15 p.m.

    ...high standard of quality control, we have built a trusted plasmid catalog containing over 150,000 sequence-verified...

15. Design Tips for Prime Editing

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    Blog Post

    Published

    Jan. 23, 2025, 2:15 p.m.

    ...tips! General principles pegRNA design If you’re just starting out with prime editing, you’ll need to ...

16. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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    Blog Post

    Published

    Nov. 14, 2024, 2:15 p.m.

    ...et al. 2021).    Figure 1: A schematic illustration of CRISPR/Cas9-based approaches to study ncRNAs...

17. Plasmids 101: Modular Cloning

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    Blog Post

    Published

    March 12, 2024, 1:15 p.m.

    ... a terminator. A simple TU could be comprised of just three parts: promoter, gene, and terminator.   ...

18. Viral Vectors 101: Calcium Sensors

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    Blog Post

    Published

    Aug. 10, 2023, 1 p.m.

    ..., one size doesn’t fit all, but never fear! That just means there is a great fit out there waiting for...

19. High Complexity Golden Gate Assembly with NEB’s DAD

    Type

    Blog Post

    Published

    Aug. 1, 2023, 1:15 p.m.

    ... a narrow search window for a site—or sites—that must be used. Each of the tools works with either 3-base...

20. Viral Vectors 101: Virus Safety

    Type

    Blog Post

    Published

    June 22, 2023, 1:15 p.m.

    ...reporting mechanisms before you start an experiment, just in case something does happen.   Finally, the best...