...carbon sources such as glycerol or glucose (in moderation) may also serve to increase cell density and ...plasmid yield. You can optimize your growth time, temperature, and shaking speed to maximize cell density and... and plasmid yield as these factors are generally, but not perfectly, correlated. Typical growth times...sufficient gas exchange in the overnight culture. Generally, large cultures in flasks may be shaken around...plates, require a faster shaking speed for proper aeration (260-300 RPM). 37℃ doesn’t work for every plasmid...plasmid Certain plasmids and strains grow best at temperatures other than the standard 37℃. This plasmid feature...recommendations to grow a plasmid at 30℃ or room temperature. These recommendations also often require a longer...

...describe and interact with the world using words like OR and AND. These logical operators are so useful...collection is the beginnings of what we think of as an operating system for engineering an entire cell. Typically...BIOFAB project is seeding the beginnings of an operating system for programming life, starting with the... pieces in these kits as the beginnings of an operating system or language for programming life. Which...as applied to products on the domestic market generates $300 billion dollars every year. That's two percent...anticipate plugging into the probiotic and cellular therapeutic space. If you were to look forward to the future...

...be stabilized by monomeric superfolded GFP and averages only ~50% RNA knockdown. In initial tests, Cas13b...Prevotella sep. P5-125 (PspCas13b) yielded 62.9% average knockdown, and they chose this enzyme for further...editing. To increase A->G editing, they employed hyperactive ADAR constructs, like ADAR2DD(E488Q). They also...and designated this system REPAIRv1. Using next-generation sequencing, they confirmed A->I editing and found...off-target activity. By mutating residues in ADAR2 that interact with duplex RNA, they hoped to destabilize ADAR-RNA...   Addressing the potential use of RESCUE in therapeutic applications, the authors tested various dRanCas13b...of RNA editing in research and its potential therapeutic uses We are also curious if REPAIR and RESCUE...

... to illuminate biology and enable next-generation therapeutics. Prime editing, base editing, PACE, and... lab places an order, they are likely to order several plasmids at once, breaking the statistical independence...our distribution, resulting in the following hierarchical model: Because the U.S., Europe, and China ...the inferred percentage of normal activity (the average rate of unique PIs/labs placing orders per day)...The inferred weekend effect is, in retrospect, generally more dramatic than the COVID-19 effect, though...not a causal interpretation -- the effect would generally not be the same if weekends were somehow imposed...

...fresh columns, regenerated columns had similar DNA yields. Additionally, columns regenerated up to five times...size of bacterial culture you’re working with. Overall, the glass syringe filter method is not only cheaper...cellulose dipstick. Image: Zou et al., 2018. Regeneration of silica columns for DNA purification Starting... and Deng labs developed a simple method for regenerating silica columns that come with PCR product purification...before a wash with ddH2O and then ethanol. After regeneration, trace amounts of DNA (3 femtograms/ul) could...Huang, L., Zhao, S., & Deng, W. (2018). Rapid Regeneration and Reuse of Silica Columns from PCR Purification...

...company that is developing RNAi therapeutics to treat neurodegenerative diseases. He has earned a B.S. ...s Board of Directors, Bart Newland. Newland is General Counsel at Atalanta, an early-stage biotech company...like? BN: I've made the transition now to being general counsel of a small start-up. For the last 20 years...and Biogen. They’re very different jobs. Being general counsel at a small start-up is very interesting...interesting. It’s very varied. There’s IP work to do, general corporate work, and negotiating contracts with other...

...base-pair mCherry insertion. Editing of these motifs generates lesions that are subject to excision and ssDNA...This edit changes the histidine to a tyrosine generating a GFP variant (GFPY66) that has a shifted emission...Reporters in Gene On include a mScarlet reporter, luciferase reporter, and neomycin resistance reporter. Image...hypothesis with GFP fluorescence. To do this they generated a silent GFP construct, that contains an ACG start... of different reporters including mScarlet-I, luciferase, antibiotic resistance markers, and other enzymes...fluorescent proteins, antibiotic resistance, and luciferase. References Gaudelli NM, Komor AC, Rees HA,...CRISPR/Cas9-mediated Genome Editing. Molecular Therapy - Nucleic Acids 5:e334 . https://doi.org/10.1038...

... the ASM General Meeting (now Microbe) in 2013 in Denver, CO. While I had been to the General Meeting ...the next generation of scientists. The connection between mentors and Singletons can accelerate the networking...meet someone new, as they’d been introduced to several people by their mentor, as well as their bin-mates...recognized by meeting organizers in their town hall.  Iterative improvement - Making Binning Singletons better...Goldschmidt conference, Coastal & Estuarine Research Federation (CERF) Biennial Conference, Gordon Research Conference...

...process to reduce viral order fulfilment from an average of 40 minutes to 10 minutes. While users can’t ...result in less than optimal user experience and the overall soundness of the software design. Each of us brings...called Scrum. The Scrum framework is based on an iterative and collaborative approach to development. Scrum...capacity to finish all of the work. Since we work in iterative chunks, we have the flexibility to adapt and easily...allows for life to happen without impacting the overall work. Each of our sprints is themed and the stories... being maintained (i.e. hasn’t been updated in several years) or they make significant changes that make...that helps our users focus on the fun stuff, accelerating research and discovery. We work with transparency...

...opportunity to discover and evolve your transferable skills. Transferable skills are the non-technical skills... 5 o’clock. You’re going to have to talk and interact with...non-scientists! Maybe you’ve just spent... Wu in graphic design. You could dread these interactions, but you could also embrace them. Talking about...production, I didn’t really start to recognize my transferable (or “soft”) skills until I was out of the lab...lab to think about these--get started with our Transferable Skills Guide.     Over the last decade, my... blog Visit our careers topic page Read our transferable skills guide Read blog posts about career readiness...