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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 848 results for: STI

Showing: 621 - 640 of 848 results

  1. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    Published
    Dec. 5, 2019, 1:49 p.m.
    ...months of strategizing, designing, developing, testing - and repeat - we’re proud and excited to finally... than through the ‘front door,’ the homepage is still important. It needs to express value, clearly communicate...

  2. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ...recombination at pseudo- or cryptic loxP sites (estimated to occur at a frequency of 1.2 per megabase in...toxicity of this enzyme in Drosophila. If you're still reading this post and you don't work in Drosophila...

  4. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ... my kids like any other youngster. His parents, Kristi and Rico, had to keep him from running too much...the fight against FOP. Second, Addgene will be hosting the AJ’s Boston League team (Contributing many ...

  6. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    Published
    March 15, 2018, 12:59 p.m.
    ...two gRNAs.  For nickase applications, a common question is: how should the gRNAs be oriented in comparison...insertions. These profiles are likely due to the distinct overhang patterns generated by the nickases; D10A...

  9. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    Published
    June 5, 2017, 3:19 p.m.
    ...for new ways to serve the research community. Questions? Feel free to reach out to us on Twitter, Facebook... Ishrat, Jane, Jason, Jessica, Joel, Jonathan, Justine, Karen, Kate, Kayla, Kelly, Kory, Kris, Leila, ...

  11. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ... but boy does it have a lot of parts! If you’re still a bit confused by CRISPR acronyms, this post is ...appears between each spacer in the CRISPR array, distinguishing the foreign sequences and keeping the array...

  13. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ...process known as phagocytosis.  Other functions We’re still learning about Fc effector functions and all that...driving particular cells or responses. And there are still antibody isotypes and Fc receptors that we don’t...

  14. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    Published
    May 4, 2023, 1 p.m.
    ...sometimes even genetic pathways that are worth investigating for drug development. Due to the difficulty ... to determine if a patient would respond to an existing therapy. In theory, CRISPR could even be used ...

  15. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    Published
    June 4, 2020, 1:22 p.m.
    ...neutralize the nuclease activity. For strains that still carry this gene, there are usually extra steps like...the pH preparation stabilizing the plasmid, and still allowing most downstream processing. On the other...

  17. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...plasmid in addition to its own chromosomal DNA, costing additional resources to maintain the plasmid. Adding...cell division (bacteriostatic); however, the distinction between the two categories may be a bit of a ...

  19. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    Published
    Jan. 22, 2019, 2:41 p.m.
    ...of red to green fluorescence allows for clear distinction between vacuolar/lysosomal and cytoplasmic Rosella...acting as an internal control. Autophagic flux is estimated by the GFP/RFP ratio: a decrease in GFP fluorescence...

  20. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    Published
    Feb. 14, 2018, 2:36 p.m.
    ... show that this basic concept of gRNA design is still challenging for researchers using CRISPR, and that...you’d like to avoid editing the genome and just stick to RNA? Cas13 may be able to help you do that! How...
Showing: 621 - 640 of 848 results