...with a 2:1 BSc in Molecular Biology (roughly a B average in the American system), in 2009 (at the beginning...structured and controlled. I worked from Standard Operating Procedures (SOP’s) and every item I tested was...sustainable. After working at this company for several years, I was ready for a change.  Working abroad...

...resonance energy transfer (FRET) is a biophysical interaction between two fluorophores. When the first fluorophore...& Zhang, 2006), quantifying antibody/antigen interactions (Saraheimo et al., 2013), and visualizing structural...different biomolecules you’d like to test for interaction. In fact, targeting a FRET fluorophore to a biomolecule...

...multiplex gRNAs, some being: Using dual nickases to generate a knockout or edit. This can help reduce off-target...gRNAs, with four being the ideal number. First you generate four unique kanamycin-resistant plasmids, each...each unused promoter. This step is necessary to generate all of the overhangs needed for the final ligation...

...Toolbox for quickly generating zebrafish transgenic lines to study neurodegenerative diseases. Listen to...

...restriction digested with PstI or EcoRV, these plasmids generate 100 bp or 1 kb DNA ladders, respectively. Unlike.... L., & Tan, S. (2017). The pPSU Plasmids for Generating DNA Molecular Weight Markers. Scientific Reports...

...hydroelectric in nature. The operation of -80C and -20C freezer temperatures, biosafety cabinet ventilation...

...viral lots. Aligning with Addgene’s mission to “accelerate research and discovery by improving access to...calculations on how to prepare a plasmid stock used to generate a standard curve, visit the AAV Titration by qPCR...

...outlets meaning you’ll have the opportunity to interact with and discuss your work with many different...applying CRISPR for mouse genome editing. Post: Generating Mouse Models Using CRISPR/Cas9   Erik L. Snapp...

...department at MIT. Crystal now works on protein therapeutics at Novartis - listen to learn more about Crystal...in industry. The importance of recognizing and leveraging your unique skills set while also adapting to...

...Genetics Institute in Cambridge, MA and Sequana Therapeutics in San Diego, CA. She earned a B.S in Biology...Addgene as the Chief Financial Officer and Chief Operating Officer. Prior to Addgene, Kenneth was an investment...

...We've also added AAV1 and AAV5 preparations of several variants of a new optogenetic tool called PdCO,... is now offering AAV1 and AAV5 preparations of several variants of a new optogenetic tool called PdCO....

...visualize the aggregated data. Plotting the data in interactive figures is a great step up, but I needed a way...access to important numbers and trends, (ii) user interaction with the data, and (iii) the ability to easily...

...perturbations to how CRISPR has made it much easier to generate mouse models.   Cpf1 Update: Comparison to Cas9...: Addgene at the ASM Microbe 2016 Conference Generating Mouse Models Using CRISPR/Cas9 Cpf1 Update: Comparison...

...the insert. pBACKBONE-Flag-hGene-EGFP Other considerations  If your insert contains a mutation or modification...be included in the plasmid name. Mutations are generally listed as the amino acid change and not a nucleotide...

...including yeast and E. coli engineering, microbial cooperation, and antibiotic production. Chapter 5: The future...before spending time in biotech screening direct interactions between drugs and promoters of oncogenes. He...

..., Addgene remains steadfast in our mission to accelerate research and discovery by improving access to...funding transitions. Enabling resource sharing: Our operating model allows researchers to access authenticated...

...who was excited about “Seeing a real lab!” And several of our visitors made sure to tell us what we already...already knew about our Shipping Operations Coordinator: “Kory was awesome!”. The students also got to ...

..., along with links to the relevant scientific literature.  -In light of the current Ebola crisis, Science... present, and future, don’t miss Carl Zimmer’s coverage on the subject in the New York Times and on his...

...modification in monkeys is invaluable for the generation of human disease models." Addgene Outreach Scientist... References, Protocols, and more References: Generation of Gene-Modified Cynomolgus Monkey via Cas9/RNA-Mediated...

...AacCas12b) exhibits optimal DNA cleavage at 48 °C, a temperature incompatible to genome editing in human cells...is a single step gRNA multiplexing system that generates vectors containing high numbers of gRNAs in one...