We narrowed to 842 results for: Eras

...and mutant loxP sites has allowed scientists to leverage this system in new, creative ways. Today’s post...potential to express the transduced reporter gene. Generating a FLEx switch to control expression of an optogenetic...Double-floxed inverse Orientation), FLEx systems are generally considered "Cre-On" meaning that your gene of ...

...specific recombination outcome using PCR and then generate a monoclonal cell line with the floxed exon. You...B, A Limaye, & AB Kulkarni. (2009) "Overview: Generation of Gene Knockout Mice". Curr Protoc Cell Biol...Basics of Homology Directed Repair Read about Generating Knockouts with CRISPR through Non-Homologous ...

...drawn to the idea of working as a team to develop therapies that could directly impact patients. And, to be...beginning of March, and the jobs are mixed into the general careers section of their web site. Each position... and is applying this towards researching neurodegenerative diseases.   Additional resources on the Addgene...

... modulators, and chimeric effectors. Nucleases generate a classic double-stranded break, while modulators...with researchers all over the world. That’s an average of 27,272 plasmids distributed per year, or approximately...it clear that it’s also a story of community, cooperation, and sharing. Sharing this story is a truly special...

...connection levels and interaction. Can bring in things to be physically interacted with. Public speaking...s much more challenging to design a hands-on interactive activity using elephants than it is to design...

...needed for EM is not something you find in the average cell and molecular biology lab, and EM is limited... is able to expand evenly in all directions to several times its original size. Depending on the protocol...depend upon the specific gel recipe used, ambient temperatures, incubation times, and the physical properties...

...Figure 1B-D). These other molecules can alter the overall 260 nm reading, distorting the final concentration...with dilute samples:  Take multiple readings to average out noisy measurements. Be cautious when interpreting...these long enough?  There are, of course, more considerations and other approaches for measuring DNA quantity...

...quick tips to get things going: If your PTA has a general meeting and a means to request funding for events...and think of a way to make this section more interactive. If you can think of a good analogy that involves...but I also tried not to be an alarmist.  The overarching guideline for all of these questions, and for...

...deprioritize genes that could be good targets for therapeutic development, particularly in cancers that are... ancestry which will reduce the likelihood of generating genetic dependency data with an ancestry-based...researching ancestry groups. And, of course, it’s imperative for scientists to be aware of and actively use...

... Prevotella and Francisella), was annotated in several genomes and is classified as a type V CRISPR system...Again, they were in for a surprise! Whereas Cas9 generates blunt ends 3 nt upstream of the PAM site, Cpf1...with Kleinstiver et al. reporting that Cpf1 can tolerate mismatches at gRNA positions 1, 8, 9, and 19-23...

...the grantor’s requirements as well as state and federal regulations. A grant professional’s job duties ...Dr. Cook served as a principal investigator for several research grants. One of the benefits of being a...management, and program evaluation are highly transferable. Use your experience and your passion(s) to ...

...scientific mentor is typically a professor who operates a research group. Within that research group, ...everything! The bare minimum should be taking notes on general protocol steps and other tips and tricks your mentor...my dissections go smoother next time?” or more generally “How am I doing? Can you tell me what’s going ...

...are below the new exemption level (the current average postdoc salary is estimated at around $45,000, ...tests must be met. In the Department of Labor’s general guide to the effect on higher education, you can...Perez, Fair Pay for Postdocs: Why We Support New Federal Overtime Rules, made clear that institutional arguments...

...sites differ at the nucleotide level but share an overall structure of 13 base pair palindromic repeats separated...efficient The first version of FLP discovered has a temperature optimum of 30 °C and is therefore inefficient...led to the identification of FLPe which has a temperature optimum of 37 °C (Buchholz, Angrand, and Stewart...

... with low affinity for ligands. When integrins interact with extracellular ligands, their ectodomains ...sites and do not interfere with integrin function. Overall, the synthetic rAbs demonstrate remarkable specificity...cells. What’s next IPI is working with Addgene to accelerate biomedical research by distributing antibodies...

...still go horribly awry when several factors aren’t taken into consideration. 1. Always focus on the science...equipment that you use in your current lab first. Generally, major equipment can be defined as equipment with...

...with a 2:1 BSc in Molecular Biology (roughly a B average in the American system), in 2009 (at the beginning...structured and controlled. I worked from Standard Operating Procedures (SOP’s) and every item I tested was...sustainable. After working at this company for several years, I was ready for a change.  Working abroad...

...resonance energy transfer (FRET) is a biophysical interaction between two fluorophores. When the first fluorophore...& Zhang, 2006), quantifying antibody/antigen interactions (Saraheimo et al., 2013), and visualizing structural...different biomolecules you’d like to test for interaction. In fact, targeting a FRET fluorophore to a biomolecule...

...multiplex gRNAs, some being: Using dual nickases to generate a knockout or edit. This can help reduce off-target...gRNAs, with four being the ideal number. First you generate four unique kanamycin-resistant plasmids, each...each unused promoter. This step is necessary to generate all of the overhangs needed for the final ligation...

...Toolbox for quickly generating zebrafish transgenic lines to study neurodegenerative diseases. Listen to...