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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 791 results for: Kin

Showing: 621 - 640 of 791 results

  1. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...fed a diet of primarily egg whites would develop skin lesions and other terrible health issues. This disease...PMC3223083. 6. McInnes, J. L., Forster, A. C., Skingle, D. C., & Symons, R. H. “Preparation and uses of...

  2. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...optimal promoter for your gene of interest, painstakingly cloned into the perfect empty backbone, made...Promoter Region – Let's Go! Mammalian Vectors Looking for More Plasmids 101 Articles?  Find them all ...

  3. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...cell types and are useful if you're going to be working in several different systems or want to transduce...j.omtn.2017.03.009 Watakabe, A., Ohtsuka, M., Kinoshita, M., Takaji, M., Isa, K., Mizukami, H., Ozawa,...

  4. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...been observed to occur in many different kinds of plasmids, taking many forms (Levene, 2009; Higgins & Vologodskii...

  5. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...gRNAs to choose from. With so many possibilities, picking a gRNA with an optimized sequence is of primary...detectable mutations (Veres et al., 2014). When working with single-cell clones, the authors note that ...

  6. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ... MQ1.) The Q147L mutation improves methylation kinetics such that cytosine methylation occurs within 24...Sander JD, Reyon D, Bernstein BE, Costello JF, Wilkinson MF, Joung JK (2013) Targeted DNA demethylation...

  8. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...with DIO/FLEx vectors and off-target expression making it easier to determine the spread pattern around...false negative or conclude the viral vector is not working.  When using a “cell-type specific” promoter, such...

  9. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...mutations are essential data points for investigators looking to definitively link genotype and phenotype. It... and do not require high sequencing read counts making them fast and viable options for multiplex sequencing...

  10. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...photographer at the Super Bowl, a medical technologist taking an x-ray, or a biologist imaging the smallest structures...microscope to use for a new sample I always start by asking two questions: 1) Is the sample dynamic or static...

  11. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...cytometry particularly well to complex experiments looking at a variety of different targets. If you are planning...43:404–409 . https://doi.org/10.1063/1.1685647 McKinnon KM (2018) Flow Cytometry: An Overview. Current...

  12. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...Base editors chemically modify DNA bases without breaking the DNA backbone, sidestepping the problem of ... 98-99% target modification in primary T cells, making them a promising tool for cell therapy applications...

  13. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...efficiency is so high that some applications may permit working with edited “pools” of cells, eliminating the time-consuming...telomere length. Telomere trimming can be assayed by looking for the production of ECT-DNA (extra-chromosomal...

  14. New Coomassie Protocol Video

    Type
    Blog Post
    Published
    Oct. 31, 2023, 1:15 p.m.
    ...Looking for a fast and inexpensive way to determine your antibody's purity? Check out our latest protocol...

  15. New Antibodies in the Lab Animation!

    Type
    Blog Post
    Published
    Nov. 19, 2024, 2:15 p.m.
    ...Thinking about using antibody-based applications in your experiment...but not sure where to start or ...

  16. Addgene Launches Partnership with IPI

    Type
    Blog Post
    Published
    June 13, 2023, 1:15 p.m.
    ...for Protein Innovation (IPI). Together, we’ll be working to provide researchers with a collection of IPI...

  17. Introducing...Addgene Quick Tips!

    Type
    Blog Post
    Published
    Sept. 19, 2024, 3 p.m.
    ...If you’re looking for the best way to improve your lab techniques — but perhaps are a little limited ...

  19. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    Published
    Dec. 2, 2016, 3:30 p.m.
    ...Addgene we’re getting into the holiday spirit by kicking off our annual #DeckTheLab contest. The bar is ...
Showing: 621 - 640 of 791 results