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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 817 results for: nin

Showing: 621 - 640 of 817 results

  1. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...the post-transcriptional additional of multiple adenine (A) nucleotides to the tail of a messenger RNA ...Promoter Controls Gene Expression Learn about New Cloning Techniques Read all of our Plasmids 101 Posts ...

  2. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...?), but there were no English speakers around. Opening talks – Genomics discoveries and moving beyond ..., over 50% of known rare diseases. One of the unintended themes of the meeting was how much we still don...

  3. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ... Epigenetic modifications play a large role in turning genes ‘off’ and ‘on’, signaling which genes should...methylation-sensitive restriction enzyme digestion, DNA adenine methyltransferase identification, and bisulfite...

  4. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ...relatively cheap). Several protocols have been written outlining the different steps and materials required for...University.  His interests include the role of learning and memory in opioid addiction and science communication...

  5. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    Published
    Jan. 21, 2015, 1:30 p.m.
    ...impolite, Complainers, who spend most of their time moaning about their troubles, Silent or unresponsive ...package your challenges with your supervisor into learning opportunities as you are advancing career, you...

  6. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ... and the team set up a directed evolution and screening experiment with three of the proteins that had...several rounds of semi-random mutagenesis and screening, the EGFP and mScarlet variants were showing no...

  7. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...groups: voltage sensitive domains and Rhodopsin containing GEVIs. Chemigenetic sensors are better classified...two-part fluorescent system, and rhodopsin still functioning as the voltage sensor. FP-rhodopsin GEVIs have...

  8. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ... the packaging cell genome, bacterial genomes, cloning vectors, and helper plasmids (Chadeuf et al., 2005... also be aligned to plasmids in our inventory containing the suspected contaminating sequence. In most...

  9. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...negative samples and, if unexpected, verified by running the product on a gel. And only ddPCR provides absolute... primer  Molecular Biology Reference Molecular Cloning Techniques References Bell AD, Usher CL, McCarroll...

  10. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...success You’ve put a great deal of thought into designing your poster – now it’s time to practice your delivery... a list of posters at all? No problem! At the beginning of the session, take a lap around the posters ...

  11. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...publications, which may take a longer time to acquire. Learning about drug discovery: Unless your academic lab...positions all at once at the end of February or beginning of March, and the jobs are mixed into the general...

  13. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...terminus the tag will end up on after successful cloning. Cloning information: This section provides useful ...

  14. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ...laboratory convenience. Disclaimer: Recommendations concerning the appropriate degree of confinement are those...Smidler, A. L., Catteruccia, F. & Church, G. M. Concerning RNA-guided gene drives for the alteration of ...

  15. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...the limitations to keep in mind whether you are planning your own ExM experiment or evaluating ExM images...expansion physically separates molecules while maintaining their relative positions, allowing you to resolve...

  16. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...favorite protein and (2) a synthetic fluorophore-contianing “ligand” that binds to the tag. Like most good...small-molecule labeling strategies is fluorogenicity, meaning the ligand exhibits low fluorescence when free ...

  17. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...  While their thorough analysis uncovered some concerning biases within widely used CRISPR screens, they...perpetuating racial bias. It is easy, even unintentionally, to attribute to an ancestry group what is ...

  18. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ... expressing neurons. We tested four serotypes containing an expression cassette with an ubiquitous promoter...be cited, tracked, and appropriately credited, broadening the knowledge base of AAV vector performance ...

  20. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ...specific genomic locus, which can require multiple cloning steps (Fujita et al., 2013). CRISPR-mediated purification... non-coding RNAs associated with telomeres by combining purification of a specific genomic region and ...
Showing: 621 - 640 of 817 results