We narrowed to 711 results for: tat
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TypeBlog PostPublishedNov. 7, 2019, 2:18 p.m.... will be, and it will need some effort and experimentation. But it ought to be possible, and if we get...
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Cultivating Community Science at BosLab
TypeBlog PostPublishedNov. 2, 2017, 1:35 p.m.... BosLab inc. as a nonprofit organization in the state of Massachusetts. Because we’re conveniently located... -
Four Factors that Differentiate the Stem Cell Field
TypeBlog PostPublishedSept. 28, 2016, 2:35 p.m.... instance in which "profound" seems like an understatement. Multiple speakers from top minds in the stem... -
5 Great Apps for Lab Life
TypeBlog PostPublishedSept. 22, 2016, 2:30 p.m.... D. Stacey is a Ph.D. student at East Tennessee State University. He works with gene expression in Pseudomonas... -
Optimizing Donor DNA for Enhanced CRISPR Genome Editing
TypeBlog PostPublishedMarch 24, 2016, 2:30 p.m....if breaking the gene is worse than leaving the mutation alone). CRISPR delivery by nucleofection The in... -
Addgene Welcomes John Doench to the Advisory Board
TypeBlog PostPublishedAug. 13, 2015, 2:30 p.m....during a lecture given by Phil Sharp. After a few rotations, Doench decided to complete his PhD work in Sharp... -
Plasmids 101: Mammalian Vectors
TypeBlog PostPublishedMarch 25, 2014, 3:15 p.m....Resistance Cell Types* Mode of Action** Working Concentation*** Blasticidin bsd HeLa, NIH3T3, CHO, COS... -
Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection
TypeBlog PostPublishedJune 25, 2019, 12:54 p.m....discovered in 1946 by Joshua Lederberg and Edward Tatum when they observed genetic recombination between... -
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME
TypeBlog PostPublishedJune 6, 2019, 12:41 p.m....population, or identify differentiation and disease states. This collection of reporter plasmids is amenable... -
Early Career Researcher Toolbox: Social Media for Scientists
TypeBlog PostPublishedDec. 11, 2018, 12:46 p.m....the three social media platforms, there’s some statistics on what percent of scientists and engineers regularly... -
A Quick Guide to a Career in Software Product Management
TypeBlog PostPublishedNov. 24, 2020, 2:15 p.m....catching up in the morning! 9:00 AM--Technical implementation review. The developers discuss which front-... -
Building and Validating Recombinant Antibodies: A Case Study
TypeBlog PostPublishedApril 5, 2022, 1:20 p.m....). Figure from Andrews, et. al., Elife A limitation of hybridoma generated mAbs is that if the frozen... -
Antibodies 101: Isotypes
TypeBlog PostPublishedOct. 19, 2021, 1:15 p.m....that helps attack extracellular pathogens. Transportation of antibodies to places they can’t reach on... -
Addgene’s Viral Service - Why Virus? Why Now?
TypeBlog PostPublishedMarch 23, 2017, 2:30 p.m....ice. The Business and Finance Teams continue to facilitate the formation of partnerships to expand the service... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog PostPublishedJune 15, 2023, 1:15 p.m....GCE4All, an NIH-funded center located at Oregon State University focused on turning GCE tools developed... -
Viral Vectors 101: Viral Applications
TypeBlog PostPublishedApril 13, 2023, 1:15 p.m.... have been engineered to introduce frameshift mutations in genes of interest, turning expression off permanently... -
Viral Vectors 101: Gamma-Retroviral Packaging Systems
TypeBlog PostPublishedAug. 6, 2024, 1:15 p.m....by long terminal repeat (LTR) sequences, which facilitate integration of the transfer plasmid sequences... -
How Addgene Creates Content
TypeBlog PostPublishedNov. 17, 2022, 2:15 p.m.... be surprised to learn that Addgene’s mission statement extends beyond plasmids, AAVs, and antibodies ... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog PostPublishedFeb. 11, 2021, 2:15 p.m....create chemogenetics receptors, GPCRs were first mutated via site-directed mutagenesis to bind non natural... -
Developing a cold tolerant E. coli using specialized chaperones
TypeBlog PostPublishedJan. 28, 2020, 2:35 p.m.... means recombinant proteins can aggregate or precipitate out as insoluble granules. We overcame this problem...