...process to reduce viral order fulfilment from an average of 40 minutes to 10 minutes. While users can’t ...result in less than optimal user experience and the overall soundness of the software design. Each of us brings...called Scrum. The Scrum framework is based on an iterative and collaborative approach to development. Scrum...capacity to finish all of the work. Since we work in iterative chunks, we have the flexibility to adapt and easily...allows for life to happen without impacting the overall work. Each of our sprints is themed and the stories... being maintained (i.e. hasn’t been updated in several years) or they make significant changes that make...that helps our users focus on the fun stuff, accelerating research and discovery. We work with transparency...

...your viral vector, you use the same protocol for generating lentivirus, but you use a different envelope ...terminals and can’t be used for neuronal tracing or peripheral gene delivery. Combining the rabies glycoprotein...Cells Are Inactivated by Human Serum. Molecular Therapy 2:218–222 . https://doi.org/10.1006/mthe.2000.0116...Modification of Hematopoietic Cells for Research and Gene Therapy. Viruses 12:1016 . https://doi.org/10.3390/v12091016...Lentiviral Vectors: One Vector, Many Guises. Human Gene Therapy Methods 28:291–301 . https://doi.org/10.1089/hgtb...transport and access to the nervous system after peripheral delivery. Human Molecular Genetics 10:2109–2121...envelope glycoprotein show increased stability in sera and augmented transduction of primary lymphocytes...

...continuous evolution and protein engineering to generate seven new prime editors, PE6a–g, that overcome...delivery and reduce off-target effects of gene therapy, Feng Zhang’s lab has developed a system called...specific cell types. It can also make CAR-T cell therapy more accessible by simplifying the production process...creation of CAR-T cells directly within the body. Overall, DIRECTED can increase on-target delivery with ... offers a promising approach for experimental therapies and medical applications.  Find DIRECTED plasmids...Lacy Proximity labeling can identify protein interactions or map a local proteome, but some applications...transcriptional history of a cell. Lin et al. generated a fusion protein called iPAK4 that assembles into...

...India at room temperature. Partnering with our distributor allowed us to ensure that temperature-sensitive...transit for a certain number of days and tracking average transit time for each courier we contracted with...destination in a timely manner and at the correct temperature. To date, we have partnered with six distributors...country. ‌For example, when we began offering temperature-sensitive materials, we noticed a large percentage...preps and libraries were kept at the appropriate temperature for the duration of their journey. Figure 2... backbones, lentiviral packaging systems, and luciferase expression. On individual material pages, we ...

...and some considerations when using the method. ELISA basics All ELISAs follow the same overall process....simple and saves time, it also has several drawbacks. Direct ELISAs generally have higher levels of background... the capture reagent to prevent non-specific interactions. Next comes the binding step where the target...Typically, the reporter is an enzyme such as horseradish peroxidase (HRP) or alkaline phosphatase (AP)... of conjugated antibodies   Additional considerations No matter what ELISA method you choose, it ...

...initiation proteins to start their own replication. In general, these plasmids tend to be low copy number. Conversely...at the low-affinity sites, the proteins work cooperatively to separate nearby AT-rich regions of DNA. In...are occupied by ATP-bound DnaA, the proteins cooperatively can separate the DNA strands. Created with BioRender.com... from the ori. In the elongation phase, a DNA polymerase synthesizes a new DNA strand at each replication...controlling the initiation of replication. Once the average copy number for a given plasmid has been reached...called iterons located near the ori and directly interacts with DnaA, stabilizing its binding and contributing...negatively regulate its own expression and thereby the overall replication of pSC101 (del Solar et al., 1998)....

...means they can be used with or as fluorophores in several different ways. Researchers have used oligonucleotides...can be created in many different ways, but the general premise is that an aptamer in its unbound state...cells. The new aptamer also has a higher melting temperature than its Spinach ancestors, and has a lower magnesium...fluorophores, including: Environments with higher temperatures, since proteins can denature while aptamers ...likely to trigger immunoresponses. Nucleotides, in general, are far less immunogenic than proteins. Assays...ions or particularly small molecules that do not generate an immune response. Aptamers can be selected for...Peixuan AND Haque, Farzin. RNA Nanotechnology and Therapeutics. Boca Raton: CRC Press, 2014. 5. Hayat, Akhtar...

...molecule into the cell. The fluorescence that is generated allows for qualitative and quantitative measurement...contact with its RNA aptamer. This increased interaction between Corn and its small molecule makes Corn...developed to quantify RNA transcription by RNA Polymerase III. By expressing Corn under promoters for transcripts... transcripts uniquely transcribed by RNA Polymerase III (5S, tRNA and U6) and measuring Corn fluorescence...effect of mTOR inhibition on the activity of RNA Polymerase III. Red and orange fluorescent aptamers Corn...changes in intracellular viral rna." Journal of General Virology 98.5 (2017): 992-1003. PubMed PMID: 28555544...PMC5602116. Song, Wenjiao, et al. "Imaging RNA polymerase III transcription using a photostable RNA–fluorophore...

...of organelles and vesicles, and more. Although several strategies are available to disrupt microtubules...extended to other GTPases. The Chow Lab has also generated a variety of membrane-recruitment optogenetic ...recently developed a full toolbox and workflow for generating new Rho GTPase optogenetic systems (Berlew et...express the other separately, then use blue light to generate CRY2-CIB1 oligomers that trap the fusion protein..., and 100 times the activity of the original, generated using directed evolution (Tei et al., 2023).  ...license.   Just make sure you don’t overdo it! The moderately-enhanced 10x and 30x SuperPLDs avoid dark-state...Krishnan, N., Ononiwu, F., Pal, D., Manikas, J., Taveras, C., Hall, N. A., Silva, J. D., Freshour, J., &...

...these techniques are cumbersome, often requiring several rounds of selection to isolate plants with the ...organisms like Arabidopsis and Medicago truncatula and several crop species including potato, corn, tomato, wheat... plant cells.   This blog post will present a general overview of plant genome engineering using CRISPR... repress target genes in plants using the same general experimental design principles developed in other...systems, multiple gRNAs can be expressed to modify several genomic loci at once (get more information on multiplexing...sequence to enhance targeting to the nucleus, and several codon optimized Cas9 variants have been created... the plasmids described above, Addgene carries several useful CRISPR toolkits for creating plant expression...