We narrowed to 842 results for: Eras

...carbon sources such as glycerol or glucose (in moderation) may also serve to increase cell density and ...plasmid yield. You can optimize your growth time, temperature, and shaking speed to maximize cell density and... and plasmid yield as these factors are generally, but not perfectly, correlated. Typical growth times...sufficient gas exchange in the overnight culture. Generally, large cultures in flasks may be shaken around...plates, require a faster shaking speed for proper aeration (260-300 RPM). 37℃ doesn’t work for every plasmid...plasmid Certain plasmids and strains grow best at temperatures other than the standard 37℃. This plasmid feature...recommendations to grow a plasmid at 30℃ or room temperature. These recommendations also often require a longer...

...describe and interact with the world using words like OR and AND. These logical operators are so useful...collection is the beginnings of what we think of as an operating system for engineering an entire cell. Typically...BIOFAB project is seeding the beginnings of an operating system for programming life, starting with the... pieces in these kits as the beginnings of an operating system or language for programming life. Which...as applied to products on the domestic market generates $300 billion dollars every year. That's two percent...anticipate plugging into the probiotic and cellular therapeutic space. If you were to look forward to the future...

...be stabilized by monomeric superfolded GFP and averages only ~50% RNA knockdown. In initial tests, Cas13b...Prevotella sep. P5-125 (PspCas13b) yielded 62.9% average knockdown, and they chose this enzyme for further...editing. To increase A->G editing, they employed hyperactive ADAR constructs, like ADAR2DD(E488Q). They also...and designated this system REPAIRv1. Using next-generation sequencing, they confirmed A->I editing and found...off-target activity. By mutating residues in ADAR2 that interact with duplex RNA, they hoped to destabilize ADAR-RNA...   Addressing the potential use of RESCUE in therapeutic applications, the authors tested various dRanCas13b...of RNA editing in research and its potential therapeutic uses We are also curious if REPAIR and RESCUE...

... to illuminate biology and enable next-generation therapeutics. Prime editing, base editing, PACE, and... lab places an order, they are likely to order several plasmids at once, breaking the statistical independence...our distribution, resulting in the following hierarchical model: Because the U.S., Europe, and China ...the inferred percentage of normal activity (the average rate of unique PIs/labs placing orders per day)...The inferred weekend effect is, in retrospect, generally more dramatic than the COVID-19 effect, though...not a causal interpretation -- the effect would generally not be the same if weekends were somehow imposed...

...fresh columns, regenerated columns had similar DNA yields. Additionally, columns regenerated up to five times...size of bacterial culture you’re working with. Overall, the glass syringe filter method is not only cheaper...cellulose dipstick. Image: Zou et al., 2018. Regeneration of silica columns for DNA purification Starting... and Deng labs developed a simple method for regenerating silica columns that come with PCR product purification...before a wash with ddH2O and then ethanol. After regeneration, trace amounts of DNA (3 femtograms/ul) could...Huang, L., Zhao, S., & Deng, W. (2018). Rapid Regeneration and Reuse of Silica Columns from PCR Purification...

...means they can be used with or as fluorophores in several different ways. Researchers have used oligonucleotides...can be created in many different ways, but the general premise is that an aptamer in its unbound state...cells. The new aptamer also has a higher melting temperature than its Spinach ancestors, and has a lower magnesium...fluorophores, including: Environments with higher temperatures, since proteins can denature while aptamers ...likely to trigger immunoresponses. Nucleotides, in general, are far less immunogenic than proteins. Assays...ions or particularly small molecules that do not generate an immune response. Aptamers can be selected for...Peixuan AND Haque, Farzin. RNA Nanotechnology and Therapeutics. Boca Raton: CRC Press, 2014. 5. Hayat, Akhtar...

...India at room temperature. Partnering with our distributor allowed us to ensure that temperature-sensitive...transit for a certain number of days and tracking average transit time for each courier we contracted with...destination in a timely manner and at the correct temperature. To date, we have partnered with six distributors...country. ‌For example, when we began offering temperature-sensitive materials, we noticed a large percentage...preps and libraries were kept at the appropriate temperature for the duration of their journey. Figure 2... backbones, lentiviral packaging systems, and luciferase expression. On individual material pages, we ...

...and some considerations when using the method. ELISA basics All ELISAs follow the same overall process....simple and saves time, it also has several drawbacks. Direct ELISAs generally have higher levels of background... the capture reagent to prevent non-specific interactions. Next comes the binding step where the target...Typically, the reporter is an enzyme such as horseradish peroxidase (HRP) or alkaline phosphatase (AP)... of conjugated antibodies   Additional considerations No matter what ELISA method you choose, it ...

...initiation proteins to start their own replication. In general, these plasmids tend to be low copy number. Conversely...at the low-affinity sites, the proteins work cooperatively to separate nearby AT-rich regions of DNA. In...are occupied by ATP-bound DnaA, the proteins cooperatively can separate the DNA strands. Created with BioRender.com... from the ori. In the elongation phase, a DNA polymerase synthesizes a new DNA strand at each replication...controlling the initiation of replication. Once the average copy number for a given plasmid has been reached...called iterons located near the ori and directly interacts with DnaA, stabilizing its binding and contributing...negatively regulate its own expression and thereby the overall replication of pSC101 (del Solar et al., 1998)....

...base-pair mCherry insertion. Editing of these motifs generates lesions that are subject to excision and ssDNA...This edit changes the histidine to a tyrosine generating a GFP variant (GFPY66) that has a shifted emission...Reporters in Gene On include a mScarlet reporter, luciferase reporter, and neomycin resistance reporter. Image...hypothesis with GFP fluorescence. To do this they generated a silent GFP construct, that contains an ACG start... of different reporters including mScarlet-I, luciferase, antibiotic resistance markers, and other enzymes...fluorescent proteins, antibiotic resistance, and luciferase. References Gaudelli NM, Komor AC, Rees HA,...CRISPR/Cas9-mediated Genome Editing. Molecular Therapy - Nucleic Acids 5:e334 . https://doi.org/10.1038...