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We narrowed to 740 results for: NSI

Showing: 321 - 330 of 740 results
  1. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ...might use it in the lab. What is a Fab Fragment? Consider the classic IgG antibody (Figure 1): it is composed...new components in your antibody tool box. As you consider which tools to use, be sure to think about what... other components of your experiment. These considerations will help you narrow down which antibody format...
  2. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ...red-shifted Ace-mScarlet, a fusion of a voltage-sensitive inhibitory rhodopsin (Ace2N) with the red fluorescent... Ace-mScarlet is a fusion between the voltage-sensitive inhibitory rhodopsin Ace2N and mScarlet. Ace2N...existing green-fluorescent GEVIs and blue-light-sensitive channelrhodopsins. Find the Ace-mScarlet voltage...
  3. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...organized lab saves time and money across the board. Consider alternative cloning strategies There are many ...methods, like Gibson assembly, are typically more expensive, so you’ll have to find a balance between time...either don’t scale or have limited scaling. The downside of high-throughput work is that you give up a ...
  4. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...antibody, this approach can greatly increase the sensitivity of an assay by increasing the number of signaling...place over multiple days. Increased run time is considered one of the major drawbacks of the indirect method...interest - the secondary antibody needs careful consideration before selection. If all the primary antibodies...
  5. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...advantage of polyclonal antibodies is that they are inexpensive. Their short production time and the bulk purification...labs. Polyclonal antibodies also have a high sensitivity to the target protein due to their ability to...or fixation (IHC). Because of their increased sensitivity, polyclonal antibodies are particularly useful...
  6. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...sequencing and qPCR methods are too costly and time intensive to process a large number of microbiome samples...bumble bee and honey bee microbiome. The toolkit consists of modular parts that are assembled with Golden...microbial population dynamics in the gut can provide insight into how the microbiome responds to infection, ...
  7. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ...using words for their field or technology in a consistent way. Just creating a lexicon was not enough. ... using the same words with the same meanings consistently, meetings will be shorter and more productive...during your postdoc that you enjoy and have a propensity for cross team communication, that’s an important...
  8. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ... dsDNA. It cleaves ssDNA with high fidelity - sensitive to even a single mismatch in the target sequence...This makes it particularly useful in generating transient edits of signaling molecules (RNA) as opposed ...for targeting mRNA and useful as a mechanism of transient knockdown of gene expression. This system could...
  9. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...that consistently receives around 200 new DNA samples every week. Here we will provide an inside look ...terminators. Detailed instructions on other factors to consider when designing your primers can be found here....
  10. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...site-specific recombination events. The system consists of two components derived from the P1 bacteriophage...cyclization recombinase”), is a 38 kDa protein responsible for intra- and inter-molecular recombination ...sites are 34-base-pair long recognition sequences consisting of two 13-bp long palindromic repeats separated...
Showing: 321 - 330 of 740 results