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We narrowed to 729 results for: NSI

Showing: 321 - 330 of 729 results

  1. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    Published
    Sept. 4, 2018, 12:53 p.m.
    ...negative regulation by using a promoter that is responsive to a repressor protein, the increase in expression... of repressor protein also increases thereby intensifying repression of the gene of interest and keeping...the increase in plasmid copy number during the transition to stationary phase, Segall-Shapiro et al. placed...

  2. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...antibody, this approach can greatly increase the sensitivity of an assay by increasing the number of signaling...place over multiple days. Increased run time is considered one of the major drawbacks of the indirect method...interest - the secondary antibody needs careful consideration before selection. If all the primary antibodies...

  3. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...advantage of polyclonal antibodies is that they are inexpensive. Their short production time and the bulk purification...labs. Polyclonal antibodies also have a high sensitivity to the target protein due to their ability to...or fixation (IHC). Because of their increased sensitivity, polyclonal antibodies are particularly useful...

  4. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ...red-shifted Ace-mScarlet, a fusion of a voltage-sensitive inhibitory rhodopsin (Ace2N) with the red fluorescent... Ace-mScarlet is a fusion between the voltage-sensitive inhibitory rhodopsin Ace2N and mScarlet. Ace2N...existing green-fluorescent GEVIs and blue-light-sensitive channelrhodopsins. Find the Ace-mScarlet voltage...

  5. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ...using words for their field or technology in a consistent way. Just creating a lexicon was not enough. ... using the same words with the same meanings consistently, meetings will be shorter and more productive...during your postdoc that you enjoy and have a propensity for cross team communication, that’s an important...

  6. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...that consistently receives around 200 new DNA samples every week. Here we will provide an inside look ...terminators. Detailed instructions on other factors to consider when designing your primers can be found here....

  7. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...site-specific recombination events. The system consists of two components derived from the P1 bacteriophage...cyclization recombinase”), is a 38 kDa protein responsible for intra- and inter-molecular recombination ...sites are 34-base-pair long recognition sequences consisting of two 13-bp long palindromic repeats separated...

  8. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    Published
    Oct. 6, 2020, 1:15 p.m.
    ...Performance When targeting single genes, SAM consistently shows the highest levels of gene activation ...which are relatively large and are not expressed consistently throughout cells. VPR Description VPR fuses ... dCas9 to activate transcription. This complex consists of the VP64 activator used in other CRISPR activation...

  9. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...position, I just had a phone interview. I also transitioned between different lab groups without having ...that have lots of general questions about job responsibilities, performance evaluations, professional development...difficulties. Have your presentation on a flash drive and consider bringing your own laptop as a backup, if the company...

  10. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...article we will discuss general viral safety considerations and best practices for handling lab-grown viruses...replicate and continue the infection cycle once inside the host. While the virus can still enter (and ...into the host’s DNA. These viruses also have a propensity to integrate into proto-oncogenes, which can ...
Showing: 321 - 330 of 729 results