We narrowed to 848 results for: STI
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TypeBlog PostPublishedJan. 22, 2019, 2:41 p.m....of red to green fluorescence allows for clear distinction between vacuolar/lysosomal and cytoplasmic Rosella...acting as an internal control. Autophagic flux is estimated by the GFP/RFP ratio: a decrease in GFP fluorescence...
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Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog PostPublishedJune 9, 2016, 2:30 p.m....world, and the question on everyone’s minds is: could NgAgo replace CRISPR? Such a drastic shift won’t happen... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog PostPublishedSept. 18, 2018, 1:33 p.m....function is inactivated by the separation of its synergistic domains. This paper demonstrated that the migration...mediated Editing (ACE) in real-time. The reporter constitutively expresses eGFP to indicate reporter abundance... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog PostPublishedNov. 12, 2019, 2 p.m....stabilization of unknown bacteria. Figure 2: Christina Mork collecting samples at the hot spring. ... Christina Mork... -
HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides
TypeBlog PostPublishedOct. 10, 2019, 12:41 p.m....zebrafish embryo over an 80 minute time course. By stitching together a stable scaffold and HA-binding regions...protein dynamics in vivo. In the future, this stitching technique could be applied to other epitopes and... -
Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC
TypeBlog PostPublishedSept. 21, 2023, 1:15 p.m....because the secondary antibody is not able to distinguish between the primary antibody and endogenous IgGs...your samples, but know that all is not lost if you still see non-specific staining! Hopefully, these solutions... -
Viral Vectors 101: What is a Virus (and a Viral Vector)?
TypeBlog PostPublishedAug. 18, 2023, 4:15 p.m.... viral vectors, which are used in the lab, are distinct from viruses — we’ll get to that in a bit! Viral...bud out, instead of lysing, but they do generally still kill the infected cell.) This is in contrast to ... -
What the HEK?
TypeBlog PostPublishedApril 18, 2023, 1:15 p.m....the fetal tissue which HEKs were isolated from is still a mystery, but it’s currently assumed that the cells...native channels. No matter what your research question, HEK-293 is known for reproducible results. It... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog PostPublishedJune 17, 2021, 1:17 p.m.... strong a signal as with the wild-type AraC, it still reached the level of expression needed for most ...bacteriograph was created using 160 individual images stitched together. Image from Romano et al., 2021 with ... -
Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource
TypeBlog PostPublishedMay 25, 2021, 2:29 p.m....have recently received a grant from the National Institutes of Health that will address this problem.The ... represent the official views of the National Institutes of Health. References and Resources References... -
Safe Port in a Storm...How Addgene is Weathering the Pandemic
TypeBlog PostPublishedSept. 17, 2020, 1:15 p.m....world mobilized on COVID-19 research, cures, and diagnostics. Almost immediately we began to receive urgent...Addgene plays in accelerating research. Yet this was still not enough to ensure we could keep up with demand... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog PostPublishedJune 2, 2020, 1:15 p.m....designed a calcineurin transcriptional reporter consisting of a quickly-degraded destabilized GFP variant...molecule-fluorescence assay to monitor restriction enzyme digestion of single molecules in real-time. Find plasmids... -
Advice for Moving Into Sales After Your Science Postdoc
TypeBlog PostPublishedAug. 21, 2014, 3:15 p.m....researchers. For many years I struggled with the questions of “Will I leave academia?” and "What type of ...the Harvard Business Review or some Chartered Institute of Marketing reviews, I would have struggled. ... -
Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System
TypeBlog PostPublishedJune 4, 2019, 1:02 p.m....methods. Unger plans to use the sensor to answer the question of whether firing of serotonergic neurons (measured... green. A second colonizer, labelled with a constitutively fluorescent reporter can also be used to track... -
Improving Transduction Efficiency with Exosome AAVs
TypeBlog PostPublishedMarch 14, 2019, 1:54 p.m....2014), but scientists have yet to decipher the mechanistic aspects behind these improvements. What have..."Enhanced liver gene transfer and evasion of preexisting humoral immunity with exosome-enveloped AAV vectors... -
5 Great Apps for Lab Life
TypeBlog PostPublishedSept. 22, 2016, 2:30 p.m....technique, I try to look for a resource to help me. I’m still waiting for a mass spectrometry app. I digress. ...one convenient resource. I can safely say that I still have the single and triple abbreviations of the ... -
Visualizing Translation at the Single Molecule Level
TypeBlog PostPublishedAug. 1, 2017, 1:15 p.m....have been able to study the effects of various stimuli on global translation, but a visual technique to... determine if proteins are targeted to their destination co- or post-translationally. TRICK: Visualizing... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog PostPublishedJuly 21, 2017, 12:48 p.m....stocks and other reagents of interest, the next question to ask is what to do with them. In some cases,... convince him to use Twitter @jonchowphd but he still reads “#” as a “pound symbol”. References 1. A. ... -
Summer SciComm Series: A PhD in Science Communication
TypeBlog PostPublishedAug. 23, 2022, 1:15 p.m....misinformation about sexual and reproductive health, as the stigma around sexual and reproductive health issues creates...never forgot about all the anxious, whispered questions from friends and classmates back in high school... -
K. phaffii: Rising to the Occasion
TypeBlog PostPublishedApril 4, 2023, 1 p.m....either fission or budding, which is the primary distinction between two of the most frequently used yeast...highly efficient at protein secretion, making harvesting recombinant proteins a cinch. This is because...