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  1. More Data for You: Find Articles Citing Addgene Plasmids

    Type
    Blog Post
    Published
    June 16, 2015, 2:15 p.m.
    ...under the "Resource Information" heading in the right column of the plasmid page. Check out the purple...can also use the "Search Table" box at the upper right of the table to search and filter the list of citing...
  2. A Conference By Postdocs For Postdocs: Future of Research

    Type
    Blog Post
    Published
    Sept. 2, 2014, 3:05 p.m.
    ... for academic research and training is getting tighter and competition is fierce. However, I believe there...conference is organized by postdocs for postdocs. I am delighted to see enterprising early career scientists taking...
  3. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    Published
    Jan. 28, 2014, 3:10 p.m.
    ... to make your own competent cells. If you need a high efficiency of transformation (for example, if you...However, you can skip this incubation step and go straight to plating if your transformed cells are being...
  4. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    Published
    Aug. 28, 2018, 12:04 p.m.
    ...‘foldscope’ (Dr. Prakash, Stanford University). Right there on the floor outside the lecture hall. It ...addition to low costs, deep tissue penetration, and high spatial resolution are benefits of this technique...
  5. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    Published
    Dec. 31, 2019, 2:26 p.m.
    ...sacB counter-selection) tools used in cloning. 8. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1... Proteins 101: GFP Fusion Proteins - Making the Right Connection by Joachim Goedhart Guest blogger Joachim...
  6. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ...experiences with CRISPR alternative, NgAgo. As you might expect with any nascent technology, researchers ...from Addgene Executive Director Joanne Kamens. I highly recommend taking a look at all of our posts as ...
  7. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    Published
    March 22, 2016, 2:30 p.m.
    ...cancers are treated with drugs designed to hit them right where it hurts, the effects are often remarkable...that confer resistance to drugs,” in a rapid, straightforward, and scalable manner, Wood said. Thankfully...
  8. From our Table to Yours: An Inside Look at Lunch at Addgene

    Type
    Blog Post
    Published
    Nov. 27, 2013, 2:54 p.m.
    ...Right from the start, when we founded Addgene nearly 10 years ago, lunch felt like a family meal. My ... we have a few unique lunchtime traditions that might be fun to incorporate into your own lab. Iron Chef-style...
  9. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    Published
    Jan. 16, 2024, 6:13 p.m.
    ...systems enable gene knockout in immune cells in a high-throughput manner, but these systems have not been...also includes pXPR_071, a plasmid that leads to higher titer lentivirus preparations than the first generation...
  10. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ...precise genome edits, the recombinases used must have high activity and specificity with low cross-reactivity...recombinase/reporter pairs were inactive, even at very high recombinase levels. These data show that multiple...
  11. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    Published
    June 10, 2014, 3:20 p.m.
    ...collaborators was extremely helpful, but with hindsight I wished I had gotten them from Addgene. I really...really struggled to get those plasmids with the right information and I believe that a lot of European ...
  12. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...signal. Reporter signals may be electrochemical or light-based, with luminescent and fluorescent reporters...transfer between the fluorescent proteins. FRET is highly amenable to biosensor creation, and custom FRET...
  13. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    Published
    Nov. 22, 2016, 3:30 p.m.
    ...leaders in the field of microbiology with multiple high-profile publications. My classmates consisted of...scientific manuscript is not prose; it is to be straight to the point. 3. Engage the reader Scientists ...
  14. New Viral Vectors - Fall 2024

    Type
    Blog Post
    Published
    Oct. 3, 2024, 1:15 p.m.
    ...monkeys it actually targets neurons, and has 45-fold higher expression levels compared to AAV9. You can now...AAV-CAG-GFP packaged in AAV9-X1.1!  We also have eight new AAV-PHP.eB vectors from the Jonathan Ting lab...
  15. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...proteins from E. coli is generally more straightforward and of a higher purity than mammalian purifications...
  16. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    Published
    Sept. 12, 2023, 1:15 p.m.
    ...mapping and design (Figure 1). Benchling: While you might think of Benchling as an electronic lab notebook...Design (DAD): This tool designs overhang sets for high complexity Golden Gate Reactions Cloning ligation...
Showing: 641 - 660 of 839 results