We narrowed to 842 results for: Eras

... the ASM General Meeting (now Microbe) in 2013 in Denver, CO. While I had been to the General Meeting ...the next generation of scientists. The connection between mentors and Singletons can accelerate the networking...meet someone new, as they’d been introduced to several people by their mentor, as well as their bin-mates...recognized by meeting organizers in their town hall.  Iterative improvement - Making Binning Singletons better...Goldschmidt conference, Coastal & Estuarine Research Federation (CERF) Biennial Conference, Gordon Research Conference...

...process to reduce viral order fulfilment from an average of 40 minutes to 10 minutes. While users can’t ...result in less than optimal user experience and the overall soundness of the software design. Each of us brings...called Scrum. The Scrum framework is based on an iterative and collaborative approach to development. Scrum...capacity to finish all of the work. Since we work in iterative chunks, we have the flexibility to adapt and easily...allows for life to happen without impacting the overall work. Each of our sprints is themed and the stories... being maintained (i.e. hasn’t been updated in several years) or they make significant changes that make...that helps our users focus on the fun stuff, accelerating research and discovery. We work with transparency...

...opportunity to discover and evolve your transferable skills. Transferable skills are the non-technical skills... 5 o’clock. You’re going to have to talk and interact with...non-scientists! Maybe you’ve just spent... Wu in graphic design. You could dread these interactions, but you could also embrace them. Talking about...production, I didn’t really start to recognize my transferable (or “soft”) skills until I was out of the lab...lab to think about these--get started with our Transferable Skills Guide.     Over the last decade, my... blog Visit our careers topic page Read our transferable skills guide Read blog posts about career readiness...

... slim chance of generating indels with base editors, keep in mind that the generation of indel sometimes...minimizes the second outcome and increases the generation of the desired T-A base pair. Figure 1...inosine), which is treated as ‘G’ by cellular polymerases. Subsequently, an original genomic A-T base pair...simultaneously.     These binary vectors could be used to generate stable genome edited plants via Agrobacterium-...transformation. Cytosine base editors for plant There are several CBEs available for plant systems. Target-AID based... transient assay. For stable transformation, regenerated plants may be subjected to genotyping. If successful...

...continuous evolution and protein engineering to generate seven new prime editors, PE6a–g, that overcome...delivery and reduce off-target effects of gene therapy, Feng Zhang’s lab has developed a system called...specific cell types. It can also make CAR-T cell therapy more accessible by simplifying the production process...creation of CAR-T cells directly within the body. Overall, DIRECTED can increase on-target delivery with ... offers a promising approach for experimental therapies and medical applications.  Find DIRECTED plasmids...Lacy Proximity labeling can identify protein interactions or map a local proteome, but some applications...transcriptional history of a cell. Lin et al. generated a fusion protein called iPAK4 that assembles into...

...means they can be used with or as fluorophores in several different ways. Researchers have used oligonucleotides...can be created in many different ways, but the general premise is that an aptamer in its unbound state...cells. The new aptamer also has a higher melting temperature than its Spinach ancestors, and has a lower magnesium...fluorophores, including: Environments with higher temperatures, since proteins can denature while aptamers ...likely to trigger immunoresponses. Nucleotides, in general, are far less immunogenic than proteins. Assays...ions or particularly small molecules that do not generate an immune response. Aptamers can be selected for...Peixuan AND Haque, Farzin. RNA Nanotechnology and Therapeutics. Boca Raton: CRC Press, 2014. 5. Hayat, Akhtar...

...company that is developing RNAi therapeutics to treat neurodegenerative diseases. He has earned a B.S. ...s Board of Directors, Bart Newland. Newland is General Counsel at Atalanta, an early-stage biotech company...like? BN: I've made the transition now to being general counsel of a small start-up. For the last 20 years...and Biogen. They’re very different jobs. Being general counsel at a small start-up is very interesting...interesting. It’s very varied. There’s IP work to do, general corporate work, and negotiating contracts with other...

... genes with potentially harmful mutations in therapeutic settings. Silencing of a target gene is achieved...RNA (shRNA) Short interfering RNA (siRNA) Piwi-interacting RNA (piRNA) miRNA and piRNA can be found endogenously...experiments. shRNA plasmids typically use RNA polymerase III promoters, which transcribe small RNAs. The..., shRNA plasmids are being designed using RNA polymerase II promoters. The design of these plasmids is...plasmids One of the most common uses of shRNAs is to generate stable knockout or knockdown cell lines. In fact...collections/mammalianrnai/","type":"EXTERNAL"},"user_generated_content":false}{% endraw %}{% end_module_attribute...(2004). MicroRNA genes are transcribed by RNA polymerase II. The EMBO Journal, 23(20), 4051–4060. https...

...molecule into the cell. The fluorescence that is generated allows for qualitative and quantitative measurement...contact with its RNA aptamer. This increased interaction between Corn and its small molecule makes Corn...developed to quantify RNA transcription by RNA Polymerase III. By expressing Corn under promoters for transcripts... transcripts uniquely transcribed by RNA Polymerase III (5S, tRNA and U6) and measuring Corn fluorescence...effect of mTOR inhibition on the activity of RNA Polymerase III. Red and orange fluorescent aptamers Corn...changes in intracellular viral rna." Journal of General Virology 98.5 (2017): 992-1003. PubMed PMID: 28555544...PMC5602116. Song, Wenjiao, et al. "Imaging RNA polymerase III transcription using a photostable RNA–fluorophore...

...of organelles and vesicles, and more. Although several strategies are available to disrupt microtubules...extended to other GTPases. The Chow Lab has also generated a variety of membrane-recruitment optogenetic ...recently developed a full toolbox and workflow for generating new Rho GTPase optogenetic systems (Berlew et...express the other separately, then use blue light to generate CRY2-CIB1 oligomers that trap the fusion protein..., and 100 times the activity of the original, generated using directed evolution (Tei et al., 2023).  ...license.   Just make sure you don’t overdo it! The moderately-enhanced 10x and 30x SuperPLDs avoid dark-state...Krishnan, N., Ononiwu, F., Pal, D., Manikas, J., Taveras, C., Hall, N. A., Silva, J. D., Freshour, J., &...