We narrowed to 740 results for: NSI

...faster assembly and disassembly dynamics, higher sensitivity to light, and better distribution of clusters...measure fluctuations of TagRFP-T fluorescence intensity due to interactions with photoactivatable fluorescent...By monitoring changes in TagRFP-T fluorescence intensity at different time points, the authors tracked ...

...significant plasmid elements. Which differences are considered a QC issue is reviewed yearly at a best practices...populations. All of these topics require additional considerations to resolve. Sequencing and assembly issues ...assembly in Geneious can still provide additional insights to resolve the assembly and determine if there...

...Performance When targeting single genes, SAM consistently shows the highest levels of gene activation ...which are relatively large and are not expressed consistently throughout cells. VPR Description VPR fuses ... dCas9 to activate transcription. This complex consists of the VP64 activator used in other CRISPR activation...

...position, I just had a phone interview. I also transitioned between different lab groups without having ...that have lots of general questions about job responsibilities, performance evaluations, professional development...difficulties. Have your presentation on a flash drive and consider bringing your own laptop as a backup, if the company...

...article we will discuss general viral safety considerations and best practices for handling lab-grown viruses...replicate and continue the infection cycle once inside the host. While the virus can still enter (and ...into the host’s DNA. These viruses also have a propensity to integrate into proto-oncogenes, which can ...

... this type of ELISA is extremely specific and sensitive to the target protein due to the use of multiple...concentrations of your protein of interest. The optical density (OD) - the measure of light absorbed by a solution...any experimental wells in that range of optical densities. If your standard curve does not have a linear...

...science and collaboration. The field already has comprehensive reference pages and curated databases for scientists... (CeNGEN) The CeNGEN goal is to establish a comprehensive gene expression atlas of every C.elegans  neuron...Scientists monitor the effects of RNAi using four-dimensional contrast interference (DIC) microscopy and computer...

... publication, Mary Dunlop’s lab generated a photosensitive Cre, Opto-Cre-Vvd, by splitting and joining.... Opto-Cre-Vvd is precise, demonstrating low sensitivity to ambient light, and versatile as it can be ...be activated by both low and high-intensity blue light. Unlike other split-Cre systems which can take up...

...fluorescent in-situ hybridization, methylation-sensitive restriction enzyme digestion, DNA adenine methyltransferase...living cells. Essentially this reporter construct consists of one substrate (the histone-derived peptide)... of these sites was controlled, the reporter consistently showed significantly different FRET levels between...

...carry out a high-throughput genetic screen, it is expensive and time consuming to individually generate gRNAs...Most insertions can only be detected by labor-intensive processes like PCR or by evaluating visual phenotypes...PMID: 27052166. Kunzelmann, Stefan, et al. “A Comprehensive Toolbox for Genome Editing in Cultured Drosophila...