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We narrowed to 729 results for: NSI

Showing: 331 - 340 of 729 results

  1. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...article we will discuss general viral safety considerations and best practices for handling lab-grown viruses...replicate and continue the infection cycle once inside the host. While the virus can still enter (and ...into the host’s DNA. These viruses also have a propensity to integrate into proto-oncogenes, which can ...

  2. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    ... this type of ELISA is extremely specific and sensitive to the target protein due to the use of multiple...concentrations of your protein of interest. The optical density (OD) - the measure of light absorbed by a solution...any experimental wells in that range of optical densities. If your standard curve does not have a linear...

  3. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ... publication, Mary Dunlop’s lab generated a photosensitive Cre, Opto-Cre-Vvd, by splitting and joining.... Opto-Cre-Vvd is precise, demonstrating low sensitivity to ambient light, and versatile as it can be ...be activated by both low and high-intensity blue light. Unlike other split-Cre systems which can take up...

  4. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...significant plasmid elements. Which differences are considered a QC issue is reviewed yearly at a best practices...populations. All of these topics require additional considerations to resolve. Sequencing and assembly issues ...assembly in Geneious can still provide additional insights to resolve the assembly and determine if there...

  5. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    Published
    Oct. 6, 2020, 1:15 p.m.
    ...Performance When targeting single genes, SAM consistently shows the highest levels of gene activation ...which are relatively large and are not expressed consistently throughout cells. VPR Description VPR fuses ... dCas9 to activate transcription. This complex consists of the VP64 activator used in other CRISPR activation...

  6. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...fluorescent in-situ hybridization, methylation-sensitive restriction enzyme digestion, DNA adenine methyltransferase...living cells. Essentially this reporter construct consists of one substrate (the histone-derived peptide)... of these sites was controlled, the reporter consistently showed significantly different FRET levels between...

  7. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...poster – now it’s time to practice your delivery. Consider your audience: Who will be attending the session... for Cancer Research Annual Meeting, you might consider including more background on how your virus of...practice: Rehearse your presentation with people inside and outside your field. Your colleagues, lab mates...

  8. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...If your poster material is nearing publication, consider uploading your work to a preprint server (and ... of the things we've mentioned above could be considered networking, but here are some more general tips...resume and prepare business cards. You might not consider being a student or postdoc a “business,” but having...

  9. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    Published
    Feb. 7, 2019, 2:16 p.m.
    ...batch analysis because of its ease-of-use and comprehensive reports. With CrispyCrunch, you get the all ...summarisation score".) For CrispyCrunch, we wanted to consider both factors, as a bench scientist would looking... our CRISPR 101 blog posts Learn about some considerations when designing gRNAs Find other CRISPR software...

  10. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...only help. Like any major life decision, the transition felt scary. While I knew two people from graduate...an industry postdoc is something that you’re considering. Like anything, there are pros and cons to postdocs... to enter academia, it is easier to make that transition from an academic setting—especially if job applications...
Showing: 331 - 340 of 729 results