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Addgene
Showing: 661 - 680 of 823 results

  1. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    Published
    July 7, 2016, 2:30 p.m.
    ... a learn a lot from these ASM pros and hope to leverage their expertise as we advance our mission of sharing...well-optimized CRISPR screening technology. Addgene got several shout-outs for making popular screening libraries...

  2. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ...aliquots could be stored indefinitely at room temperature (thus also saving freezer space).  The procedure...BitesizeBio is the protocol for making your own Taq polymerase by Outreach Scientist, Andy Baltus. Addgene founder...

  3. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    Published
    Jan. 16, 2014, 2:56 p.m.
    ...develop general technical proficiency, communicate your work, maintain ethical standards and interact professionally...

  4. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...viruses bud out, instead of lysing, but they do generally still kill the infected cell.) This is in contrast... Taylor (Ed.), Viruses and Man: A History of Interactions (pp. 23–40). Springer International Publishing...

  5. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...depositors. Below you’ll find brief summaries of several excellent guest posts from our depositors. Interested...Harvard University, describes R-bodies, “force generating protein machines that can extend and retract ...

  6. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    Published
    Jan. 22, 2019, 2:41 p.m.
    ...being targeted for autophagy. The development of several fluorescent autophagy reporters now allows for ...translation, this fusion protein is cleaved to generate one molecule of GFP-tagged LC3 for every one molecule...

  9. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ... Repeats of Adeno-Associated Virus. Human Gene Therapy Methods 30:206–213 . https://doi.org/10.1089/hgtb...basic viral vector elements Read about four considerations for the perfect AAV tool Learn about synthetic...

  10. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type
    Blog Post
    Published
    Sept. 5, 2019, 1:06 p.m.
    ...The Golden Stopwatch Award highlights TTOs that accelerate science by processing agreements quickly! If ...your institution’s TTO or legal office first. Generally, Addgene is also waiting to receive that legal...

  11. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ...of knowledge or interest in STEM subjects; we generally refer to them as the “interested public.” Subscribers... have a higher level of science knowledge than average and are more likely to spend time engaging with...

  12. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ... plants (Arabidopsis floral dip). In the next generation the CRISPR construct needs to be segregated away...transformants can be produced. In the following generation when CRISPR needs to be removed to prevent further...

  13. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ...Plasmids 101: Cre-lox Cre recombinase can be used to generate inversions, deletions, and translocations. It ...Joining So you know that CRISPR can be used to generate knockouts, but how does it work? Guest contributors...

  14. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ... cellular behaviors often fall short with the generation of various flux imbalances. The conventional ...authors sequentially rerouted the metabolic flux to generate differently pigmented derivatives of violacein...

  15. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...organizations in 111 countries. From 2019 to 2023, we averaged facilitating 89,477 MTAs per year.  The MTA Process...the process, from notifications and emails to generating the UBMTA link for the recipient organization...

  16. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ...if in the end all they want to do is to quickly generate a single cell line and proceed to use it for experiments...researcher must amplify the library, use next-generation sequencing to ensure that the library has retained...

  17. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ...should recommend #PhysicalDistancing. Social interactions and #social support are even more necessary ...while. Getting started early on introductions and literature reviews could make the months leading up to your...

  18. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ... plasmids deposited at Addgene still accrue on average 2 to 3 times as many citations as other papers ...could be growing more quickly which could also generate this type of correlation. But even with that caveat...

  19. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...effectively is now your new measure of success. It is imperative that you resist the temptation to “just do it...of the team to ensure that no component of the overall project gets left undone or is duplicated. With...
Showing: 661 - 680 of 823 results