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We narrowed to 839 results for: igh

Showing: 661 - 680 of 839 results

  2. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ... I settled on using glass slides. With just the right amount of agarose, I could pour a very thin gel ...surface tension.  It's a bit tricky to pour the right amount so the agarose doesn’t pour over the sides...

  3. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    Published
    Jan. 16, 2014, 2:56 p.m.
    ...period. While there are many aspects to choosing the right labs (see my webinar (updated link from 2020) on...Mentoring" Don’t ignore warning signs If lab members, neighbors or alumni give you information about the PI that...

  8. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ..., is species compatible, and doesn’t come with a high background can be a huge challenge. Epitope tags...amino acids. FLAG tag has been used with moderate to high success rates across all applications. You can find...

  10. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    Published
    Nov. 17, 2016, 3:30 p.m.
    ...for similarly controlling neuronal activity with light will be available soon. We’re also distributing ...controls to determine if your AAVs are getting to the right cells using your particular delivery technique and...

  11. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...protocol takes about 2-3 weeks but requires less than eight hours worth of hands-on time. Creating a modular... the SEC-based system is both simple to use and highly efficient for C. elegans genome modification. ...

  13. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...). They saw that these AAV particles come in a slightly different shape. These AAV particles are often...purified AAV vectors. They found that they have a higher transduction efficiency, i.e. the same number of...

  14. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    Published
    Jan. 12, 2021, 2:15 p.m.
    ...out of the incubator because I’d had a baby that night, all he said was “OK” and hung up on me). I went... 25 years since our last contact, but we were delighted to be reconnected.  Science can be a small community...

  15. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    ...dry, and then view under an oil immersion using a light microscope. Gram positive bacteria will be stained...decolorization. In contrast, counterstain, safranin, is a light and water soluble dye that is able to enter the ...

  16. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ...can't be too hard to apply. These papers suggest it might work well enough to consider as a therapeutic.” ...handle on how cleavage sites are repaired.” Those insights will be critical for scientists’ ability to influence...

  17. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...makes NEBuilder HiFi so accurate (apart from the high-fidelity polymerase) is the ability to remove, reset...covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc...

  18. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ...focus on discussion and brainstorming Though, we might be forced to work from home and/or be in the lab...experience more interactive. The button is on the right of the Share Screen control panel on the top of ...

  19. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ...great way to take a mental break, while meditating right before bed will help you prepare for sleep. At Addgene... Home. It’s normal and okay to feel overwhelmed right now, but hopefully these tips are helpful to you...
Showing: 661 - 680 of 839 results