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Showing: 661 - 680 of 831 results

  1. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ..., is species compatible, and doesn’t come with a high background can be a huge challenge. Epitope tags...amino acids. FLAG tag has been used with moderate to high success rates across all applications. You can find...

  3. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...protocol takes about 2-3 weeks but requires less than eight hours worth of hands-on time. Creating a modular... the SEC-based system is both simple to use and highly efficient for C. elegans genome modification. ...

  4. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...makes NEBuilder HiFi so accurate (apart from the high-fidelity polymerase) is the ability to remove, reset...covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc...

  6. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...). They saw that these AAV particles come in a slightly different shape. These AAV particles are often...purified AAV vectors. They found that they have a higher transduction efficiency, i.e. the same number of...

  9. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ...great way to take a mental break, while meditating right before bed will help you prepare for sleep. At Addgene... Home. It’s normal and okay to feel overwhelmed right now, but hopefully these tips are helpful to you...

  10. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    ...dry, and then view under an oil immersion using a light microscope. Gram positive bacteria will be stained...decolorization. In contrast, counterstain, safranin, is a light and water soluble dye that is able to enter the ...

  11. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ...can't be too hard to apply. These papers suggest it might work well enough to consider as a therapeutic.” ...handle on how cleavage sites are repaired.” Those insights will be critical for scientists’ ability to influence...

  12. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ...focus on discussion and brainstorming Though, we might be forced to work from home and/or be in the lab...experience more interactive. The button is on the right of the Share Screen control panel on the top of ...

  13. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    Published
    Jan. 12, 2021, 2:15 p.m.
    ...out of the incubator because I’d had a baby that night, all he said was “OK” and hung up on me). I went... 25 years since our last contact, but we were delighted to be reconnected.  Science can be a small community...

  14. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...videos. The search function in the manual just highlights what the user searches, which is not very helpful...

  15. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ...next steps (see “action items” below). Get the right people in the room There are few experiences more...informative, and pleasant. Email: Sometimes, the right meeting is no meeting at all. When no discussion...

  16. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...bandwidth to become an expert) the end result is high quality and the entire process is completed more... What types of issues do you want to know about right away? Provide adequate support. The manager must...

  18. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...is typically cytotoxic, which makes generation of high titer Cas9 lentiviruses challenging. This cytotoxicity... Bähr M. Human synapsin 1 gene promoter confers highly neuron-specific long-term transgene expression ...

  19. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...middle) and extends from the 3' end of the nick (right), occasionally introducing mutations (red nucleotide...EvolvR with a targeted mutagenesis rate ~9-fold higher than the original nCas9, while increasing the standard...

  20. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    Published
    Dec. 11, 2018, 12:46 p.m.
    ...you that Twitter is a tool worth using, but you might be wondering how to get started. This blog post ...video from Science Media Savvy At this point, you might be be thinking social media could be useful, but...
Showing: 661 - 680 of 831 results