We narrowed to 702 results for: lic

1. Hot Plasmids and Viral Preps - May 2021

   Type

   Blog Post

   Published

   May 18, 2021, 1:15 p.m.

   ...acid, suggesting that MPyV system can improve metabolic pathway yields by encapsulating a single enzyme...

2. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

   Type

   Blog Post

   Published

   July 13, 2017, 2:30 p.m.

   ... from mutations in the gene, and whether any publications have mentioned the gene. The Bloomington Drosophila...

3. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

   Type

   Blog Post

   Published

   June 22, 2017, 2:30 p.m.

   ...fluorescent protein to your organelle of interest. Click on the gene names in the figure below to find plasmids...

4. Fluorescent Proteins 101: Fluorescent Protein Timers

   Type

   Blog Post

   Published

   May 4, 2017, 2:30 p.m.

   ...-FT-N1 pTRE-Slow-FT pBAD/HisB-Slow-FT -  Applications of fluorescent protein timers FP timers have...

5. Which Fluorescent Protein Should I Use?

   Type

   Blog Post

   Published

   May 20, 2014, 2:06 p.m.

   .... al. (2010) “Fluorescent proteins and their applications in imaging living cells and tissues”  Physiol...

6. CRISPR 101: Making a Knock-In Cell Line

   Type

   Blog Post

   Published

   Dec. 22, 2022, 2:15 p.m.

   ...nutrients in their media, they will not undergo DNA replication, so make sure you are keeping an eye on those...

7. Uncovering Ancestral Bias in CRISPR Research

   Type

   Blog Post

   Published

   Nov. 29, 2022, 2:15 p.m.

   ...same reference genomes and libraries. Further complicating the issue is the long and ugly history of reporting...

8. CRISPR 101: RNA Editing with Cas13

   Type

   Blog Post

   Published

   July 31, 2020, 12:30 p.m.

   ...Addressing the potential use of RESCUE in therapeutic applications, the authors tested various dRanCas13b truncations...

9. AAVs CREATed for Gene Delivery to the CNS and PNS

   Type

   Blog Post

   Published

   Sept. 28, 2017, 2:01 p.m.

   ...morphologic studies), as well as translational applications (mechanistic studies and gene therapy treatments...

10. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type

    Blog Post

    Published

    Oct. 4, 2018, 12:44 p.m.

    ...Calabrese pooled library and lentiviral prep here! Applications for the Root and Doench CRISPR libraries Overall...

11. Summer SciComm Series: Modes of Communication

    Type

    Blog Post

    Published

    July 1, 2022, 1:15 p.m.

    ...bring in things to be physically interacted with. Public speaking is a common fear. Can be discouraging ...

12. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type

    Blog Post

    Published

    Aug. 9, 2016, 2:30 p.m.

    ...therapeutics. We’ll have more to say about clinical applications of CRISPR in the next section. In order to denote...

13. The Importance of a Fun Workplace: Company Culture at Addgene

    Type

    Blog Post

    Published

    Sept. 3, 2015, 2:30 p.m.

    ...be an obvious one, but don’t make things too complicated for yourself (especially if you are the sole ...

14. Plasmids 101: Protein tags

    Type

    Blog Post

    Published

    Dec. 11, 2014, 4:26 p.m.

    ...deciding on which one to use will depend on your application (see table below). The antibodies available for...

15. Negotiating Work and Life: How to Find the Joy

    Type

    Blog Post

    Published

    March 20, 2014, 3:04 p.m.

    ...can make a decent meal, fold their laundry, take public transportation, call a cab and remember their own...

16. CRISPR 101: Any Base Transversion Editors

    Type

    Blog Post

    Published

    April 1, 2025, 1:15 p.m.

    ...researchers can now select the best tool for their application from among many options.  Conclusion With these...

17. Twenty Years of Sharing: Expanding Accessibility

    Type

    Blog Post

    Published

    Nov. 21, 2024, 10:47 p.m.

    ...Systems AAV Plasmids Blogs Origin of Replication What is a Plasmid? How to Design Your gRNA for...

18. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type

    Blog Post

    Published

    July 30, 2024, 1:15 p.m.

    ...approach and involves reading primary literature, soliciting information from requestors and depositors, and...

19. Antibodies 101: The Four ELISAs and When to Use Them

    Type

    Blog Post

    Published

    July 25, 2024, 1:15 p.m.

    ...). Fluorescent tags may also be used, but for simplicity we will discuss the more common enzyme-based ...

20. How to Be an Excellent Trainee

    Type

    Blog Post

    Published

    Sept. 26, 2023, 1:15 p.m.

    ...tell a trainee is understanding and things are ’clicking’ for them. It’s often easiest to train individuals...