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Showing: 661 - 680 of 704 results

  1. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...affect your AAV experiments. Luckily, the other posts in our Viral Vectors 101 series can help guide you...axonal transport  (Murlidharan et al., 2014), and postsynaptic transmission (Oh et al., 2020). For example,...Others, like AAV1 and AAV9, can also transduce postsynaptic cells when used at very high titers (Zingg et...

  5. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...This post was contributed by guest blogger Søren Hough, a Biochemistry PhD candidate at the University...

  12. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...What's more, depositing is free! We've written this post as a step-by-step guide to the online deposit process...

  15. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...you’ll still have to lyse your proteins. For this post, we are assuming that you are running a denaturing...

  16. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ... combinations. Check out our retrograde AAV blog post. An additional desirable property for network visualization...

  17. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    Published
    Oct. 7, 2014, 3 p.m.
    ...a sense of relief that I attended the amazing, postdoc-driven Future of Research Conference at Boston ...fantastic city-wide effort was led by two amazing postdocs, Kristin Krukenberg and Jessica Polka from Harvard...relieved because it finally feels to me like the postdocs are taking some steps to control their own future...feckless”.  So what can be done?  He charged the postdocs to “Grab ahold of your destinies” and also suggested...tractable” problems.  We must better define the postdoc (and even the grad student) trainee experience ...Anderson-Thompkins, Director of the Office of Postdoctoral Affairs at UNC, called her presentation “Disequilibrium...Disequilibrium, Disillusion and The Postdoc Dilemma” which pretty much said it all.  She cited the oft ...

  18. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    Published
    Jan. 15, 2015, 1:50 p.m.
    ...Interested in Addgene's Keystone Poster?  We'll be sharing the poster with our blog readers and on social...meeting participants. They were the ones holding poster tubes (or as our baffled flight attendant called..., & more via Addgene Addgene's addition to the poster session was a first for our repository, and I was...expect in terms of interest, but visitors to the poster were impressed to see how the numbers of plasmid... in the past 4 years. I kept busy for the whole poster session, talking to people from every conceivable...all the Addgenies who worked so hard to make this poster, offered suggestions and tips, and helped get me...

  19. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ...data from postdoctoral programs in the Boston area that demonstrate that 85% of leaving postdocs pursue ...especially important for graduate students and postdocs to get a perspective on other types of science...Both explicit and implicit aspects of today’s postdoctoral training can directly interfere with a seamless...

  20. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...recently it hadn’t been used to treat disease postnatally. Now, three papers published concurrently in ...shown CRISPR can treat a genetic disease in a postnatal mouse model, an important proof of concept for... that CRISPR and AAV can be used together for postnatal genome editing in mice. Could this approach work...Bhattacharyya S, Shelton JM, Bassel-Duby R, Olson EN. Postnatal genome editing partially restores dystrophin expression...
Showing: 661 - 680 of 704 results