We narrowed to 718 results for: tat

1. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

   Type

   Blog Post

   Published

   Dec. 8, 2016, 3:30 p.m.

   ...actually contain your insert of interest. Simply stated, ccdB makes cloning easier by selecting against...

2. Developing Lab Management Software for Biology

   Type

   Blog Post

   Published

   Aug. 25, 2016, 2:30 p.m.

   ... track the location, quality, growth, and legal status of thousands of plasmids a day, like we do at Addgene...

3. Gendered Innovations: Why Does Sex of the Cell Matter?

   Type

   Blog Post

   Published

   July 21, 2016, 2:30 p.m.

   ...Gendered Innovations. This project: 1) develops state-of-the-art methods of sex and gender analysis and...

4. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

   Type

   Blog Post

   Published

   Sept. 26, 2019, 12:50 p.m.

   ... The team found that "all-in-one" Nanoblades facilitated HDR with either a ssDNA or dsDNA donor template...

5. Adenoviral Vector Production and Troubleshooting

   Type

   Blog Post

   Published

   June 14, 2018, 11:44 a.m.

   ...ratio for the purified virus should be ~1.3 Quantitative PCR: the number of viral DNA packaged in virions...

6. Plasmids 101: Modular Cloning

   Type

   Blog Post

   Published

   March 12, 2024, 1:15 p.m.

   ... allows users to test a variety of different orientations and components within the system to maximize...

7. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

   Type

   Blog Post

   Published

   Dec. 14, 2021, 2:15 p.m.

   ...without clearly testing whether a certain methylation state causes a condition. To understand mechanisms and...

8. CRISPR in the Clinic

   Type

   Blog Post

   Published

   Nov. 2, 2023, 1:15 p.m.

   ...targeted to precisely edit with the goal of ‘fixing’ mutations or introducing a desired edit, but this particular...

9. Viral Vectors 101: Calcium Sensors

   Type

   Blog Post

   Published

   Aug. 10, 2023, 1 p.m.

   ...switches from green fluorescence to a red fluorescent state only by simultaneous (1) user-controlled illumination...

10. Antibodies 101: Choosing the Right Isotype

    Type

    Blog Post

    Published

    April 6, 2023, 3 p.m.

    ...that require high affinity binding, such as immunoprecipitations. Your sample species should also be considered...

11. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type

    Blog Post

    Published

    July 12, 2021, 1:15 p.m.

    ...now circulating and plasmids containing these mutations have been deposited at Addgene. Plasmids deposited...

12. Hot Plasmids and Viral Preps - March 2021

    Type

    Blog Post

    Published

    March 23, 2021, 1:15 p.m.

    ...vivo studies should always take DNA’s chromatin status into account (Fig 1a). The laboratory of Karmella...

13. Plasmids for Endogenous Gene Tagging in Human Cells

    Type

    Blog Post

    Published

    April 6, 2017, 1:02 p.m.

    ...it’s also good to make a few single base pair mutations in crRNA target sites found within the homology...

14. 5 Tips for Troubleshooting Viral Transductions

    Type

    Blog Post

    Published

    Aug. 11, 2016, 2:23 p.m.

    ... to a target cell by reducing the repulsive electrostatic forces between these two negatively-charged ...

15. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type

    Blog Post

    Published

    July 14, 2016, 2:30 p.m.

    ...sophisticated genome-wide analysis. At many of its computationally predicted off-target sites, Cpf1 does not mediate...

16. Plasmids 101: Terminators and PolyA signals

    Type

    Blog Post

    Published

    March 31, 2016, 2:30 p.m.

    ...sequence conserved Rho-independent termination annotation for 90 bacterial elements. By Ppgardne at English...

17. How-to: 5 Steps to a Great Panel Discussion

    Type

    Blog Post

    Published

    Oct. 22, 2014, 1:57 p.m.

    ...use any slides for the event. Slides are for presentations, not discussions. I will rarely work with a ...

18. Tips to Make the Most of a Scientific Conference

    Type

    Blog Post

    Published

    May 23, 2019, 12:26 p.m.

    ...themselves and then ask questions. Practice your presentation for both instances. If your poster material ...

19. 10 Basic tips for mammalian cell culture

    Type

    Blog Post

    Published

    July 12, 2018, 1:09 p.m.

    ...their comfort zone around 37 °C and 5% CO2 and get agitated with sudden changes in temperature (especially...

20. Transferable Skills: Negotiation

    Type

    Blog Post

    Published

    March 3, 2022, 2 p.m.

    ...more important to you and why? What are their expectations/needs and how are they different from mine? ...