We narrowed to 711 results for: tat

1. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

   Type

   Blog Post

   Published

   Sept. 26, 2019, 12:50 p.m.

   ... The team found that "all-in-one" Nanoblades facilitated HDR with either a ssDNA or dsDNA donor template...

2. Plasmids for Endogenous Gene Tagging in Human Cells

   Type

   Blog Post

   Published

   April 6, 2017, 1:02 p.m.

   ...it’s also good to make a few single base pair mutations in crRNA target sites found within the homology...

3. Adenoviral Vector Production and Troubleshooting

   Type

   Blog Post

   Published

   June 14, 2018, 11:44 a.m.

   ...ratio for the purified virus should be ~1.3 Quantitative PCR: the number of viral DNA packaged in virions...

4. CRISPR in the Clinic

   Type

   Blog Post

   Published

   Nov. 2, 2023, 1:15 p.m.

   ...targeted to precisely edit with the goal of ‘fixing’ mutations or introducing a desired edit, but this particular...

5. Viral Vectors 101: Calcium Sensors

   Type

   Blog Post

   Published

   Aug. 10, 2023, 1 p.m.

   ...switches from green fluorescence to a red fluorescent state only by simultaneous (1) user-controlled illumination...

6. Antibodies 101: Choosing the Right Isotype

   Type

   Blog Post

   Published

   April 6, 2023, 3 p.m.

   ...that require high affinity binding, such as immunoprecipitations. Your sample species should also be considered...

7. Plasmids 101: Modular Cloning

   Type

   Blog Post

   Published

   March 12, 2024, 1:15 p.m.

   ... allows users to test a variety of different orientations and components within the system to maximize...

8. Hot Plasmids and Viral Preps - March 2021

   Type

   Blog Post

   Published

   March 23, 2021, 1:15 p.m.

   ...vivo studies should always take DNA’s chromatin status into account (Fig 1a). The laboratory of Karmella...

9. 5 Tips for Troubleshooting Viral Transductions

   Type

   Blog Post

   Published

   Aug. 11, 2016, 2:23 p.m.

   ... to a target cell by reducing the repulsive electrostatic forces between these two negatively-charged ...

10. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type

    Blog Post

    Published

    July 14, 2016, 2:30 p.m.

    ...sophisticated genome-wide analysis. At many of its computationally predicted off-target sites, Cpf1 does not mediate...

11. Plasmids 101: Terminators and PolyA signals

    Type

    Blog Post

    Published

    March 31, 2016, 2:30 p.m.

    ...sequence conserved Rho-independent termination annotation for 90 bacterial elements. By Ppgardne at English...

12. How-to: 5 Steps to a Great Panel Discussion

    Type

    Blog Post

    Published

    Oct. 22, 2014, 1:57 p.m.

    ...use any slides for the event. Slides are for presentations, not discussions. I will rarely work with a ...

13. Transferable Skills: Negotiation

    Type

    Blog Post

    Published

    March 3, 2022, 2 p.m.

    ...more important to you and why? What are their expectations/needs and how are they different from mine? ...

14. Tips to Make the Most of a Scientific Conference

    Type

    Blog Post

    Published

    May 23, 2019, 12:26 p.m.

    ...themselves and then ask questions. Practice your presentation for both instances. If your poster material ...

15. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type

    Blog Post

    Published

    Sept. 12, 2017, 1:44 p.m.

    ...is Addgene plasmid #44362. Method validation, limitations, and future plans How did AAV-68544 become contaminated...

16. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type

    Blog Post

    Published

    May 4, 2017, 2:30 p.m.

    ...Associated Plasmids from Subach et al. Protein Excitation (nm) Emission (nm) Brightness pKa Maturation ...

17. 10 Basic tips for mammalian cell culture

    Type

    Blog Post

    Published

    July 12, 2018, 1:09 p.m.

    ...their comfort zone around 37 °C and 5% CO2 and get agitated with sudden changes in temperature (especially...

18. Viral Vectors 101: Voltage Indicators

    Type

    Blog Post

    Published

    Jan. 30, 2024, 2:15 p.m.

    ...of the previously described systems. Several adaptations of this method have been to tag, cage, uncage...

19. Hot Plasmids: Winter 2025

    Type

    Blog Post

    Published

    March 11, 2025, 1:15 p.m.

    ... and subthreshold events, including miniature excitatory postsynaptic potentials (mEPSPs) (Hao and Lee...

20. Common Injection Routes in Mice

    Type

    Blog Post

    Published

    Jan. 22, 2025, 9:42 p.m.

    ...finally, to expert.   Figure 1. Visual representation of common injection routes in mice. Image created...