...for a higher number of MPE. Therapeutic potential  To explore the therapeutic potential of the DAP array...pathogen. Over the past decade, CRISPR tools have leveraged such host-defense mechanisms to enable multiplex...to develop a strategy that would independently generate multiple gRNAs for nCas9-BE, or any other kinds...drive-and-process.” To optimize DAP use, they developed a generalizable method to acquire the most effective DAP array... demonstrated up to 31-loci MBE with over 50% averaging editing efficiency in human cells. MBE with DAP...

...part of my job, in addition to preparing DNA and generating/replenishing kits, is to find new ways to make...extract libraries from previously fragmented DNA.The general process for library preparation using this kit ...total of about 3 hours. It was robust, capable of generating a wide range of insert sizes and also produced...participants from different biotech companies to interact and learn more about one another. They also provided...representatives. I got to talk with a technician from Neon Therapeutics, a biotechnology company that uses neoantigen...extremely helpful since she was familiar with library generation. Also, I now know what to expect at a workshop...

...remote collaborations Transferable Skills Guide: External collaborations Transferable Skills Guide: Teamwork...how to seek out such an arrangement, and how to operate once in that position. What is a visiting scientist...new placement. Make sure you read any relevant literature for the techniques you will be performing to ...home institute. Discuss rights Do you expect to generate data that will contribute to a publication? A ...authorship and rights for anything that could be generated during the visit. If you don’t feel comfortable...

...2012). The polarizing impact of science literacy and numeracy on perceived climate change risks. Nature... between what I was doing in the lab and the interactions I was having outside of the “ivory tower.” I... acute “adeolitis” – received the best ratings overall, simply because none of the participants had preconceived...show that people with greater levels of science literacy are especially prone to this, possibly because...Fischhoff, B. (2017). Individuals with greater science literacy and education have more polarized beliefs on controversial...

... In each case, we leveraged the team’s knowledge of the scientific domain in general and of our data in... that we needed to improve more than that: the general “findability” of the information on the site. Different...approached the issue from multiple angles: the overall user experience of our website, the different website...resources and handle millions of queries. In an iterative process driven by our user experience experts,...approach much more than if we were dealing with general purpose information. Below are a few of the strategies...

...proteomics--the study of the distribution and interactions of proteins within these structures. Enzyme-...substrate to map the spatiotemporal distribution and interaction networks of specific proteins in living cells...process is much slower and can take as long as several days. In 2015, the laboratory of long-time Addgene..., the enzymes function across a much broader temperature range than BioID, which only functions optimally...modular domains that facilitate protein-protein interactions and mediate biochemical processes. One of the...than previously thought. Sidhu’s group has made several hundred SH3 domain containing plasmids available...

... these interactions, and can give valuable insight into DNA expression and protein interactions, including...technique that provides insight into protein:DNA interactions. ChIP works by using antibodies to capture protein... focuses on the DNA your protein is actively interacting with, reducing background information. Your options...could mean needing to increase crosslinking to counteract the effects of increased sample agitation; a ... this investment can well be worth it once you generate your data. However, it may be a good idea to add...

...synthetic) pFZ19 (yellow flame) Temperature inducible promoters Temperature sensitive expression systems ...Promoters control the binding of RNA polymerase and transcription factors. Since the promoter region ...Types of inducible promoters Chemical agents, temperature, and light are all examples of factors that can...promoters; they show near-zero expression at regular temperatures but can be induced by heat or cold exposure....since expression can be repressed by glucose. Temperature inducible promoters are also known for their ...

... systems that measure biological phenomena by generating an output which is directly proportionate to ...antibody, enzyme, DNA, etc. The receptors job is to generate a quantitative signal upon recognizing the analyte... (fluorescence, pH, etc.). The transducer will generate a numerical value which represents the amount ...disciplines! Most of the sensors designed for calcium generate a fluorescent output, which will turn on, change... It’s a good thing, though! Viral delivery has several advantages over other mechanisms. For one, many...permanently integrate and/or express long term (up to several years!). Biosensors tend to be more reproducible...

...more effectively used in a technology or as a therapeutic.  A (very) brief history GCE came onto the synthetic...specific to the ncAA and orthogonal to (i.e. non-interacting with) the cells’ own natural translation machinery...system (including media, cell type, and growth temperature) may need to be individually optimized for each...focused on turning GCE tools developed in the literature into robust and universal techniques that researchers...fulfill their mission. While they’ve shortlisted several protocols, including their phosphoserine ncAA protocol...., Griffith, M. C., & Schultz, P. G. (1989). A General Method for Site-specific Incorporation of Unnatural...