We narrowed to 842 results for: Eras

...these techniques are cumbersome, often requiring several rounds of selection to isolate plants with the ...organisms like Arabidopsis and Medicago truncatula and several crop species including potato, corn, tomato, wheat... plant cells.   This blog post will present a general overview of plant genome engineering using CRISPR... repress target genes in plants using the same general experimental design principles developed in other...systems, multiple gRNAs can be expressed to modify several genomic loci at once (get more information on multiplexing...sequence to enhance targeting to the nucleus, and several codon optimized Cas9 variants have been created... the plasmids described above, Addgene carries several useful CRISPR toolkits for creating plant expression...

...highly multiplexed tissue imaging method that uses iterative staining and chemical bleaching to allow for high-resolution...high-quality antibodies and reagents that you know work…preferably before you start your experiment. (And you’re...the IBEX Knowledge-Base, seeking to create an iterative process that is constantly becoming better, with...Ichise, H., Gola, A., Kabat, J., Lowekamp, B., Speranza, E., Croteau, J., Thakur, N., Jonigk, D., Davis...Hernandez, J. M., & Germain, R. N. (2022). IBEX: An iterative immunolabeling and chemical bleaching method for...00644-9 Radtke, A. J., Kandov, E., Lowekamp, B., Speranza, E., Chu, C. J., Gola, A., Thakur, N., Shih, R...

...to three) working in your chosen field. This cooperative education experience (co-op) is what has brought...find out that the two hours were split up between several Addgene employees, who all made the daunting task...old) employees with information on how Addgene operates as a team and shows how they fit into the system... on projects there. At this point, I have seen several lab processes and am currently working on projects...are scheduled to take place and employees are generally expected to be available. In addition to these...student at Northwestern University. She is one of several students completing their co-op at Addgene. This...

...overlapping or nested on a register, then the operation of one recombinase on the register could affect...affect the way downstream recombinases interact with the register- either by re-orienting their recognition...amplified by a particular primer pair. Then, by leveraging qPCR data from multiple primer pairs, one can...designed to have 20-30 nt length, ~60 oC annealing temperature, and ~50% GC content) so that individual parts...not – the state machine design space is highly degenerate and often times there are multiple register designs...serine recombinases have been described in the literature (5, 6) and may be substituted for one another...predictability of RSMs and recombinase-based circuits in general would benefit from a more comprehensive characterization...

... to the molecule it detects. This change then generates a reporter signal. Reporter signals may be electrochemical...fluoresce; when present, calcium induces CaM/RS20 interaction, thereby changing the FP’s structural organization...M13. When calcium binds, it triggers CaM/M13 interaction that increases FRET transfer between the fluorescent..., and voltage across membranes. These sensors generally follow the same pattern described above - a sensing...withstand a particular solute concentration or temperature? For further advice, see our posts on how to ... Plate-based assays (plate reader) Detect the average amount of fluorescence across a population of cells...

...the relevant literature to understand where the field is today? Have you talked to veterans in the lab ...-to-date with the literature of a couple of well-known journals that discuss general science in the area... timeline? A typical research project can take several years to complete and has multiple benchmarks. ...before even touching a pipetteman. Take the time to generate new ideas and think about them critically before...

...looking to determine how Protein A and Protein B interact. You read extensive research on the two proteins... both targets in your specimen. You scour the literature and find an ideal antibody against Protein A ...reduce immunogenicity and thereby enhance the therapeutic potential of antibody medicines. In this process...with human antibody sequences.  Additional considerations Although isotype conversion is routinely used...utility of recombinant antibody tools, the resulting chimera may have different binding properties (Morelock...needs to be addressed when multiplexing. It is imperative that you use narrowly selective isotype-specific...

...previously yielded valuable enzymes. Famously, Taq polymerase – from Thermus aquaticus bacterium – was discovered...processing environmental samples Since the high temperatures and unstable ground around these hot springs...collection site, we took GPS coordinates, water temperature readings using a heat gun, and pH measurements... continued profiling samples, and years later, several whole genome sequencing projects were launched ...Parvibaculum lavamentivorans, whose optimal growth temperature is 30°C. Find IgnaviCas9 here! What can you do...applications where reagents must be stored at elevated temperatures for long time periods and workflows where CRISPR...

...Thailand, our annual operating budget is approximately 20,000 USD and we are working on several genetic diseases...: This year, the BeHEARD (Helping Empower and Accelerate Research Discoveries) Award attracted submissions...affected by AHC suffer from recurrent attacks of unilateral paralysis (hemiplegia) that are often painful...stressors, including emotional stress or changes in temperature.  “The disease was first described in 1971,” ...elucidating association between SOX10 and WS4 and also several other rare genetic diseases. We hope kids in Thailand...

...outcrossed knock-in ready for experimentation. In 2014, several manuscripts published in Genetics detailed approaches... DNA as repair templates which reduces time to generate edits to only four days (Paix et al., 2017). The... demonstrated that selection for repair of a temperature-sensitive pha-1 mutation significantly enriches...design The Meyer lab meticulously characterized several aspects of editing. First, they devised guidelines...modifying the 5’ modification of oligos used to generate linear dsDNA repair templates by PCR boosted efficiency...from a single injected animal.  PAM sequence considerations For changes 5’ to the PAM, sequences corresponding...elegans The Meyer and Mello labs also identified several experimental set ups that could improve CRISPR ...