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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 837 results for: Eras

Showing: 701 - 720 of 837 results

  1. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...auxotrophic selection in E. coli? Additional considerations HIS3 L-hisitidine no S. cerevisiae  no yes...ade6+ purine (adenine)   S. pombe  no no   Considerations when using auxotrophic selection Of course,...

  2. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...human embryos has been met with a storm of media coverage. But the paper published August 2nd in Nature ...present.   Another issue surrounding CRISPR-based therapy is the level of testing needed to define whether...

  3. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...individual colors to 6. A gRNA with all three hairpins generates white light. All 6 gRNAs can be expressed in a...separate vector. Figure 2: CRISPRainbow generates 6 unique colors and a white composite. gRNAs with...

  5. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...  Prime editing improvements By Angela Holmes Several labs have recently shared some exciting plasmid...systems improve gene editing performance at therapeutically relevant gene targets and expand the application...

  6. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...plasmids, such as special growth instructions (temperature, strain, media supplements), suggested sequencing...re like us, you need a strategy for processing several sequencing reactions in parallel. We have a few...

  8. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...and synthetic biology Synthetic biologists have leveraged the power of Golden Gate cloning into a modular...cloning or Gibson assembly. Another important consideration is the design of flanking overhangs. Although...

  10. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ... of society or devoid of social context. This “general public” audience comprises the taxpayers who fund...be affected by the knowledge and applications generated from this research. At both a practical and ethical...

  12. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    ...binding biotin), is conjugated to the enzyme horseradish peroxidase (HRP). Finally, the substrate of HRP... mx+b?). Figure 5: A standard curve is generated by plotting OD against known concentrations of...

  14. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...folks at the makerspace comfortable with next-generation sequencing data and the bioinformatics pipeline...resources to pursue it. Since makerspaces are generally funded on a membership model - everyone contributes...

  15. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...purposes, but mainly for the delivery of various therapies and viral vectors. This injection sounds very ... that require ingestion, such as experimental therapies or pain relief, straight to the stomach. This ...

  16. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...timers may be sensitive to changes in oxygen, temperature, and pH, but timer activity can be calibrated...dynamics in a synthetic circuit (Saxena et al.) In a general sense, FP timers can be used in any situation where...

  17. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...Also, avoid unnecessary talking because talking generates microbe-laden aerosols that may enter the hood... CO2 and get agitated with sudden changes in temperature (especially if you have air conditioning on) ...

  18. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...other departments/laboratories? How long is the average PhD in this department? How has the department ...research focuses on Drosophila-parasite pathogen interactions.  ...

  19. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...protein family. What sets them apart is the way they generate their color: they strongly absorb visible light...hydrophilic amino acids to reduce non-specific interactions with cytosolic proteins. This step is important...

  20. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ... I needed help with: wouldn’t it be fun to get several of the labs in our program to write short, funny...of content they share and produce and how they interact with their followers. It’s also been a great place...
Showing: 701 - 720 of 837 results