We narrowed to 842 results for: Eras

...serotype also has promising therapeutic applications; it can enhance therapeutic delivery across broader ...

... to pursue their scientific curiosity but can literally put their work on the shoulders of giants. The... and blueprints has the potential not only to accelerate scientific progress, but also to enable science...

...(MFI) of the different populations. MFI is the average brightness from all cells that are positive for...background in protein mutagenesis and cancer immunotherapies. She is currently a Research Assistant in Arlene...

...CRISPR gene editing methods relied solely on Cas9. Overall, these approaches have two main drawbacks: 1) ...plasmids; for multiple edits, single plasmids are generally more efficient. Pro-Tip Make sure to reverse the...

...expressing recombineering proteins Red/ET from a temperature-sensitive plasmid. Homologous recombination then.... Using commercially available high-fidelity polymerases such as KOD and Phusion, which are recommended...

...they examined gRNA expression levels and indel generation for two PTG constructs in rice protoplasts. Compared...human cells. Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK. Mol Cell. 2014 May 22;54(4):698-710. ...

...auxotrophic selection in E. coli? Additional considerations HIS3 L-hisitidine no S. cerevisiae  no yes...ade6+ purine (adenine)   S. pombe  no no   Considerations when using auxotrophic selection Of course,...

...  Prime editing improvements By Angela Holmes Several labs have recently shared some exciting plasmid...systems improve gene editing performance at therapeutically relevant gene targets and expand the application...

...allowing an influx of ions into the neuron which generates an action potential (Figure 1). This light-induced..., must be optimized simultaneously in order to generate a channelrhodopsin that’s successful in vivo. ...

...individual colors to 6. A gRNA with all three hairpins generates white light. All 6 gRNAs can be expressed in a...separate vector. Figure 2: CRISPRainbow generates 6 unique colors and a white composite. gRNAs with...

...human embryos has been met with a storm of media coverage. But the paper published August 2nd in Nature ...present.   Another issue surrounding CRISPR-based therapy is the level of testing needed to define whether...

...plasmids, such as special growth instructions (temperature, strain, media supplements), suggested sequencing...re like us, you need a strategy for processing several sequencing reactions in parallel. We have a few...

...GBPb-GAL4 (DNA-binding domain); and a UAS-driven luciferase reporter construct. In the absence of GFP, no...create a complete transcription factor, activating luciferase transcription. Tang et al. coined this system...

...and synthetic biology Synthetic biologists have leveraged the power of Golden Gate cloning into a modular...cloning or Gibson assembly. Another important consideration is the design of flanking overhangs. Although...

...through programming cells. In the context of therapeutics, if you want to program a cell to identify and...Sometimes in CRISPR people talk about off-target interactions. That’s relevant if you want to make sure you...

...binding biotin), is conjugated to the enzyme horseradish peroxidase (HRP). Finally, the substrate of HRP... mx+b?). Figure 5: A standard curve is generated by plotting OD against known concentrations of...

...monoclonal hybridomas, but those techniques have several drawbacks. Polyclonal antibodies produced in animals...antibodies are monoclonal antibodies that are generated in vitro using synthetic genes that are typically...

...recognized that genome sequencing projects were generating huge amounts of data that could provide the basis... to genes in other species, but these studies generally leave questions about gene function wide open....

... of society or devoid of social context. This “general public” audience comprises the taxpayers who fund...be affected by the knowledge and applications generated from this research. At both a practical and ethical...

...folks at the makerspace comfortable with next-generation sequencing data and the bioinformatics pipeline...resources to pursue it. Since makerspaces are generally funded on a membership model - everyone contributes...