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Addgene

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We narrowed to 729 results for: NSI

Showing: 361 - 370 of 729 results

  1. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...of the desired T-A base pair. Figure 1: Transition mutation (C>T, G>A, A>G, and T>C) installation...also constructed smaller vectors suitable for transient assays using protoplast transformation. Cytosine...72 hours post-transformation/transfection in a transient assay. For stable transformation, regenerated ...

  2. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    Published
    Feb. 18, 2020, 2:15 p.m.
    ... bins together at a mixer. We call this Quorum Sensing, after the phenomenon in bacteria that occurs when...gene expression in response to changes in cell density. At the mixer, they meet other Mentors and Singletons...briannadoesbio) June 22, 2019 Favorable conditions - Expansion of networks The last stage is Exponential Growth...

  3. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ... can be to get a “bad batch”); Customizable sensitivity and detection - since they frequently undergo...fluorescence for readout may render this approach less sensitive in most applications. Learn About Fluorescent ... sensor could save precious minutes in a time sensitive detection assay. In 1999, Grate and Wilson paired...

  4. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...clone. You will also need to use a E. coli strain sensitive to CcdB (e.g. DH5α, TOP10, Mach1). The ccdB gene... the CcdB protein inhibits the growth of CcdB sensitive E. coli strains, most colonies should contain ...sequences, start codon, stop codons, reading frame considerations, etc). This video demonstrates how to use the...

  5. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...When choosing beads, there are several factors to consider. Agarose beads are sponge-like and vary in shape...express the protein of interest, such as a sample transiently transfected or stably infected with the protein...appealing, but the disadvantages must be carefully considered before using. For example, the tag may affect...

  6. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ... the virus and is less affected.  Altering sensitivity to serum. Different pseudotyped viruses are differentially... protein that binds phosphatidylserine and low-density lipoprotein receptors (LDLRs) on a cell surface...CD, Friedman T, McClure MO, Weiss RA (1997) Sensitization of rhabdo-, lenti-, and spumaviruses to human...

  7. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...pTargetF system consists of one plasmid that expresses the S. pyogenes Cas9, a temperature-sensitive replicon...changed the replicon of pCas into a non-temperature-sensitive replicon, and 3) added the sacB gene for counterselection...

  8. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...place and give you some reasons why you should consider using Reddit to share your science. While Reddit...permanent reference by the Winnower. The only small downside to the AMA from my end as an organizer/promoter... general advice on how to meet people, how to transition out of academia, and other science career topics...

  9. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...in the door When I was first considering preparing for and transitioning to a career in healthcare consulting...fast turnover of projects, pressure to maintain consistently high levels of performance, and occasionally...

  10. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...staining and the molecular weight and relative intensity of the viral capsid proteins are analyzed. The...preparation shows that the vast majority of the vectors consist of full particles (white arrowhead) relative to...targeting integrated copies of the lentiviral Rev responsive element (RRE) gene and the cellular ribonuclease...
Showing: 361 - 370 of 729 results