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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 837 results for: Eras

Showing: 721 - 740 of 837 results

  1. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ...conclusively shown that barrier protocols are vulnerable to human error. And with gene drives, one mistake... M. Concerning RNA-guided gene drives for the alteration of wild populations. eLife e03401 (2014). doi...

  2. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...process has changed with the introduction of next generation sequencing!  Choosing a BLAST program Of the...amount of time to perform the search as well as the overall alignment results. The default setting is “Optimize...

  3. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...7 mutations found in the REC2, REC3, and PAM interacting domains and allows for expanded PAM recognition...engineering was Cas12a (formerly Cpf1), a nuclease that generates double strand breaks in target genes resulting...

  4. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...remotely-tethered ligands (PORTLs). OptoXRs are chimeras of the extracellular domain of endogenous light-sensitive...Created with BioRender.com.   Important considerations for using optogenetic tools Once you’ve selected...

  5. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    .... During FACS, the sample stream oscillates to generate droplets that are charged as they pass through... cells. Once in the cells, amine reactive dyes interact with free amines in the cytoplasm causing dead...

  8. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    Published
    May 13, 2019, 12:01 p.m.
    ...CRISPR overview and history. Tuesday will focus on generating knockouts using CRISPR and Wednesday will highlight...

  9. The 12 Days of CRISPR

    Type
    Blog Post
    Published
    Jan. 3, 2023, 2:15 p.m.
    ...variants for targeted RNA degradation with minimal collateral effects🎵 On the 1st day of CRISPR, Addgene gave...

  11. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    Published
    Feb. 4, 2015, 3:58 p.m.
    ...adoption of the Cas9 technology was also greatly accelerated through a combination of open-source distributors...

  12. CRISPR 101 eBook...en español

    Type
    Blog Post
    Published
    Jan. 18, 2024, 2:15 p.m.
    ...¡La primera edición de nuestro libro electrónico CRISPR 101 ya está disponible en español! Esta traducción...

  14. New Videos: Addgene Lab Tips

    Type
    Blog Post
    Published
    Feb. 15, 2018, 2:41 p.m.
    ...reagents to a single tube and simply flick the tube several times. This will help ensure ensure equal distribution...

  17. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    Published
    Feb. 1, 2024, 2:15 p.m.
    ... 2020. We find this process takes 4-6 weeks on average. Therefore, we recommend placing your order well...

  20. Deck the Lab 2022!

    Type
    Blog Post
    Published
    Jan. 17, 2023, 2:15 p.m.
    ... pic.twitter.com/rExV3b8Q8u— Michael Früh (@Michelderapfel) December 19, 2022   Congratulations to all...
Showing: 721 - 740 of 837 results