We narrowed to 792 results for: Kin
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TypeBlog PostPublishedDec. 8, 2016, 3:30 p.m....protein (CcdB) that acts as a DNA gyrase poison, locking up DNA gyrase with broken double stranded DNA and...
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Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening
TypeBlog PostPublishedApril 12, 2016, 2:30 p.m.... has revolutionized how we decode the genome by making it easy to create specific genetic perturbations... -
Addgene's Tips for Plasmid Quality Control
TypeBlog PostPublishedJan. 14, 2016, 3:30 p.m.... will depend on the particular plasmid you are working with, but, in most cases at Addgene, we check what... -
Plasmids 101: Cre-lox
TypeBlog PostPublishedJan. 13, 2015, 3:47 p.m....types, during certain stages of development, or by making the Cre inducible (such as with tamoxifen or doxycycline... -
A Look Back at One Year of Plasmid Sharing for COVID-19 Research
TypeBlog PostPublishedJuly 12, 2021, 1:15 p.m....HY, Tortorici MA, Veesler D, Murphy M, Pettie D, King NP, Balazs AB, Bloom JD (2020) Protocol and Reagents... -
Overcoming the Challenges of Lentiviral Production
TypeBlog PostPublishedAug. 7, 2018, 12:31 p.m....tips and tricks for overcoming these hurdles. Talking 'bout my (lentiviral) generation The first step... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog PostPublishedMarch 8, 2017, 3:15 p.m....process. Figure 1: Begin the deposit process by clicking “Submit Online Deposit”or “Download Deposit Spreadsheet... -
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog PostPublishedSept. 26, 2019, 12:50 p.m....Nanoblades? They’re easily produced in a BSL2 lab, making them a versatile and accessible genome editing ... -
Open Resources and Plasmid Tools For Studying C. elegans
TypeBlog PostPublishedJuly 18, 2019, 12:55 p.m....associate, self-excise, and covalently ligate their flanking peptides together (Wang et al., 2018).” With the... -
CRISPR in the Clinic
TypeBlog PostPublishedNov. 2, 2023, 1:15 p.m....pipelines (viral), and researchers are currently working on giving this treatment a CRISPR spin. CRISPR ... -
Antibodies 101: Choosing the Right Isotype
TypeBlog PostPublishedApril 6, 2023, 3 p.m....References Lu, Q. L., & Partridge, T. A. (1998). A New Blocking Method for Application of Murine Monoclonal Antibody... -
A Look at Addgene's QC Process
TypeBlog PostPublishedMay 6, 2025, 1:15 p.m....ensure the entire team is using the same rubric and making the same decisions when analyzing the wide variety... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog PostPublishedOct. 8, 2024, 1:15 p.m....of conjugation, and these are encoded by certain kinds of plasmids — more on that in a minute! Fun fact... -
Progress Towards a PAM-Free CRISPR
TypeBlog PostPublishedMarch 7, 2024, 2:39 p.m....mean it’s suitable for your experiment. If you’re looking for more pan-genome PAM flexibility, you’ll want... -
Plasmids 101: Terminators and PolyA signals
TypeBlog PostPublishedMarch 31, 2016, 2:30 p.m....eBook to get all the background you need to start working with plasmids! What are termination and polyadenylation... -
PCR Applications for Copy Number Variation Assays
TypeBlog PostPublishedApril 26, 2022, 1:15 p.m....relative quantification - that is, since you’re looking at the amount of DNA in your sample relative to... -
Plasmids 101: Protein Expression
TypeBlog PostPublishedJune 7, 2018, 1:17 p.m....antibodies and pharmaceuticals, specifically interleukins. Yeast expression systems Yeast are a great ... -
Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria
TypeBlog PostPublishedApril 4, 2019, 12:53 p.m....gene in the recipient's genome. Antibiotic plates lacking DAP select against donor bacteria and for recipients... -
No Llamas Required - Synthetic Nanobodies Against Membrane Proteins
TypeBlog PostPublishedJune 18, 2020, 1:15 p.m....membrane-bound spike protein, in a remarkable 12 working days. While the lab used this method for COVID-... -
Plasmids 101: Degron Tags
TypeBlog PostPublishedMarch 14, 2024, 1:15 p.m....round of evolution (AID-2) to reduce some of the leakiness associated with the system, but the general concept...