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Fluorescent Proteins 101: Aptamer Fluorophores

Type

Blog Post

Published

April 11, 2017, 2:30 p.m.

...or as fluorophores in several different ways. Researchers have used oligonucleotides in fluorescent visualization...
Plasmids 101: Gateway Cloning

Type

Blog Post

Published

Jan. 12, 2017, 3:30 p.m.

... genes, including Hs.KRAS4a. Use our website to search for your favorite gene! STEP 2: Generate an Expression...
Getting the Most from Your Lentiviral Transduction

Type

Blog Post

Published

April 7, 2016, 2:30 p.m.

...Resources on Addgene.org Find Viral Vectors for Your Research Brush Up on All Things Lentivirus with our Lentiviral...
New Optogenetic Tools for Cytoskeleton and Membrane Control

Type

Blog Post

Published

Sept. 28, 2023, 1:15 p.m.

...more to optogenetics than neuronal activity. Researchers have designed a variety of photoswitchable elements...
The Fluorescent Vegetables in Aptamer Soup

Type

Blog Post

Published

Jan. 29, 2019, 2:27 p.m.

... instead activates Spinach fluorescence. In the search for something more stable for quantitative imaging...
Plasmids 101: Biotinylation

Type

Blog Post

Published

Nov. 15, 2018, 1:50 p.m.

...ssDNA or RNA–protein interactions.” Nucleic Acids Research, 23.18 (1995): 3792–3793. PubMed PMID: 7479014...
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

Type

Blog Post

Published

Sept. 1, 2020, 1:15 p.m.

...approaches developed in the early 2000’s which allow researchers to deliver oversized cargo (Hirsch et al., 2010...
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

Type

Blog Post

Published

March 1, 2016, 3:30 p.m.

... with Linkers (MODAL) strategy." Nucleic acids research 42.1 (2014): e7-e7. PubMed PMID: 24153110. PubMed...
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

Type

Blog Post

Published

April 24, 2015, 2:39 p.m.

...systems. Interested in applying Brainbow to your research? Check out Brainbow plasmids available from Addgene...
Viral Vectors 101: AAV Serotypes and Tissue Tropism

Type

Blog Post

Published

Jan. 16, 2025, 3:42 p.m.

...numbered AAV1, AAV2, and so on. In addition, researchers have created many engineered serotypes by mixing...
Antibodies 101: The Basics of Western Blotting

Type

Blog Post

Published

March 8, 2021, 2:41 p.m.

... that you can make on your own. To narrow your search, first pay attention to the validated applications...
Technique: Probe Phage Genomes for Host Binding Proteins

Type

Blog Post

Published

Sept. 29, 2016, 2:30 p.m.

...the (all-too-common) event that homology-based searches return no candidates. Our strategy: Express random...
Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

Type

Blog Post

Published

March 7, 2017, 3:30 p.m.

...pretty amazing. Despite the shifting landscape of research opportunities, it’s an exciting time to be in ...
Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

Type

Blog Post

Published

Dec. 15, 2016, 3:57 p.m.

...blogger, Beth Kenkel Beth Kenkel is currently a research scientist in the Department of Laboratory Medicine...
Antibodies 101: Designing Your First Flow Panel

Type

Blog Post

Published

May 28, 2024, 1:45 p.m.

...design your first flow panels! Paul Heisig is a Research Associate in the lab of Arlene Sharpe at Harvard...
Which Fluorescence Microscopy Techniques is Best for Me?

Type

Blog Post

Published

Oct. 10, 2017, 1:57 p.m.

...of tissue clearing techniques (9) now allows researchers to image tissues that are over a cm3 in size....
Technical Design of a Western Blot

Type

Blog Post

Published

Aug. 22, 2024, 1:15 p.m.

...modifications, we recommend doing a little more research into gel chemistry and/or specialty gel options...

Showing: 761 - 777 of 777 results

Results per page:

Fluorescent Proteins 101: Aptamer Fluorophores

Plasmids 101: Gateway Cloning

Getting the Most from Your Lentiviral Transduction

New Optogenetic Tools for Cytoskeleton and Membrane Control

The Fluorescent Vegetables in Aptamer Soup

Plasmids 101: Biotinylation

Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

Viral Vectors 101: AAV Serotypes and Tissue Tropism

Antibodies 101: The Basics of Western Blotting

Technique: Probe Phage Genomes for Host Binding Proteins

Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

Antibodies 101: Designing Your First Flow Panel

Which Fluorescence Microscopy Techniques is Best for Me?

Technical Design of a Western Blot

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