We narrowed to 800 results for: Des

1. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

   Type

   Blog Post

   Published

   July 23, 2020, 1:20 p.m.

   .../35++ Adenovirus Vector Expressing Anti-CRISPR Peptides Decreases CRISPR/Cas9 Toxicity in Human Hematopoietic...

2. Antibodies 101: Stripping and Reprobing Western Blots

   Type

   Blog Post

   Published

   Aug. 1, 2024, 1:15 p.m.

   ...v) HCl Adjust to pH 2.0–2.2 For the mildest stripping solution, omit the Tween 20. Once your...

3. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

   Type

   Blog Post

   Published

   Aug. 24, 2021, 1:15 p.m.

   ...ELISA is a modification of the sandwich ELISA as described here, the direct and indirect ELISA (more details...

4. Meet an Addgenie: Insights into Grad School, Career Paths, and More

   Type

   Blog Post

   Published

   June 25, 2020, 1:15 p.m.

   ... I can have on an organization beyond my job description.” Our resident bagel provider, source of energy...

5. Engaging with science and society at pgEd

   Type

   Blog Post

   Published

   Sept. 20, 2018, 12:17 p.m.

   ...uncomfortable places, from sticky ethical dilemmas to dark episodes in the history of eugenics and research misconduct...

6. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

   Type

   Blog Post

   Published

   June 26, 2018, 1:33 p.m.

   ...guanidine moiety at the end of its side chain. Decades ago, other labs discovered that the in vitro functions...

7. Adenoviral Vector Production and Troubleshooting

   Type

   Blog Post

   Published

   June 14, 2018, 11:44 a.m.

   ...for faster detection of RCA. 2nd Gen AdVs were designed to have increased cloning capacity, decreased  ...

8. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

   Type

   Blog Post

   Published

   May 1, 2018, 1:57 p.m.

   ...specific protein-protein interactions, SplitBioID provides a candidate list of proteins that are part of ...

9. Lab Automation at Addgene

   Type

   Blog Post

   Published

   March 22, 2018, 1:28 p.m.

   ...Biology Tips from the Repository Trenches: Using Barcodes to Track Samples Resources at Addgene.org Find...

10. 6 Tips for Grant Writing

    Type

    Blog Post

    Published

    Oct. 18, 2016, 2:30 p.m.

    ...to a lay audience. My lay paragraph typically includes a couple of sentences of introduction to my area...

11. The Materials Science of Optogenetics Experiments

    Type

    Blog Post

    Published

    Sept. 17, 2015, 2:30 p.m.

    ...materials required for each [1, 2]. This protocol [3] provides, in exquisite detail, the various steps required...

12. Gaining Leadership Skills Volunteering at a Professional Organization

    Type

    Blog Post

    Published

    Aug. 6, 2015, 2:30 p.m.

    ...communication and learning to work with people despite the distance improved my verbal and written communication...

13. Experimenting in an Open Source lab: from CRISPR to Cats

    Type

    Blog Post

    Published

    July 23, 2015, 2:30 p.m.

    ...Derek Jacoby from Makerspace Victoria, CA Over the decades science has become increasingly restricted to academic...

14. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type

    Blog Post

    Published

    Jan. 21, 2015, 1:30 p.m.

    ...familiar with the minute details of your work. Describe the problem by stating the facts and explain how...

15. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type

    Blog Post

    Published

    July 2, 2020, 12:40 p.m.

    ...Variant for Your Next Experiment Learn More About Designing gRNAs Against Your Favorite CREs Here Additional...

16. Production of Virus in Insect Versus Mammalian Cells

    Type

    Blog Post

    Published

    Nov. 5, 2024, 8:32 p.m.

    ... (about three to four days), this second round provides the added benefit of producing a stock P1 virus...

17. Magnetic Control of Proteins: More than a Dream

    Type

    Blog Post

    Published

    Sept. 24, 2024, 1:15 p.m.

    ...Maria were encouraged by previous reports that described magnetic responses of cryptochrome proteins or...

18. Antibodies 101: Flow Compensation

    Type

    Blog Post

    Published

    May 16, 2024, 1:15 p.m.

    ...This will make it difficult to properly gate your desired cell population. By compensating between fluorescent...

19. Hot Plasmids: Winter 2025

    Type

    Blog Post

    Published

    March 11, 2025, 1:15 p.m.

    ...Optimized dicot prime editing enables heritable desired edits in tomato and Arabidopsis. Nature Plants,...

20. Transferable Skills: Negotiation

    Type

    Blog Post

    Published

    March 3, 2022, 2 p.m.

    ...specific details about the individuals, each example provides a scenario where both people get something valuable...