We narrowed to 902 results for: Ide
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TypeBlog PostPublishedApril 19, 2016, 2:30 p.m....to not produce artifacts in your experiments. Considerable effort has gone into making FPs monomeric, yet..., increasing the probability that two FPs will collide with each other. The consequences can be dramatic...localized to the endoplasmic reticulum, the normally spiderweb-like pattern of tubules can be grossly distorted...
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Droplet Digital PCR for AAV Quantitation
TypeBlog PostPublishedJan. 16, 2020, 2:18 p.m....factor of 2. This means that if you set up two identical assays with the same sample, you could end up ... should fall within the range of the assay for a wide range of titers. We usually dilute our samples 1...experiments. References Furuta-Hanawa, Birei, Teruhide Yamaguchi, and Eriko Uchida. "2D droplet digital... -
Plasmids 101: Gateway Cloning
TypeBlog PostPublishedJan. 12, 2017, 3:30 p.m.... codon, stop codons, reading frame considerations, etc). This video demonstrates how to use the Snapgene...Protocols Watch Our Diagnostic Restriction Digest Video Check Out Our Molecular Biology Reference Pages ... -
Mesothelioma - Causes, Symptoms, and Treatment
TypeBlog PostPublishedDec. 13, 2016, 3:30 p.m....more funding and research opportunities are being provided to immunotherapy than ever before. Although different...from many other types of cancer. Mesothelioma is considered an orphan cancer; it is rare disease with only...(ADAO) are working toward such a ban, and the president of the organization, Linda Reinstein – whose husband... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog PostPublishedOct. 19, 2017, 1:54 p.m....sites. FRT sites and loxP sites differ at the nucleotide level but share an overall structure of 13 base...37 °C). Smart molecular evolution led to the identification of FLPe which has a temperature optimum of ...Recombination between the two target sites with identical sequence leads to inversion of the flanked DNA... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog PostPublishedMarch 5, 2019, 1:08 p.m....fluorescence can occur. Cre recombinase can be used alongside common, existing tools for fly research that use...turnover of Langerhans cells in the dermis and identify that Langerhans cells are replenished through ...The intestinal stem cell markers Bmi1 and Lgr5 identify two functionally distinct populations." Proceedings... -
Deep Mutational Scanning with One Pot Saturation Mutagenesis
TypeBlog PostPublishedFeb. 22, 2017, 3:30 p.m....results in a polymerase error rate of up to 2% per nucleotide position. The “sloppy” PCR reaction is created...ssDNA is packaged into phage and then extracted. Besides taking a couple days to generate the phage-derived...strand: A mix of degenerate primers and the high-fidelity Phusion polymerase are used to introduce point... -
New Antibodies in the Lab Animation!
TypeBlog PostPublishedNov. 19, 2024, 2:15 p.m....found the video radd-ically helpful! More resources at Addgene Addgene's Antibody Guide What is an... -
Addgene Launches Partnership with IPI
TypeBlog PostPublishedJune 13, 2023, 1:15 p.m....Innovation (IPI). Together, we’ll be working to provide researchers with a collection of IPI’s well-validated...on our website and blog, including our antibody guide, antibody protocols, and Antibodies 101 blog series... -
Introducing...Addgene Quick Tips!
TypeBlog PostPublishedSept. 19, 2024, 3 p.m.... And you’ll be able to find all published videos in our Quick Tips playlist: Want your quick tips...be sharing our favorite parts of every quick tip video on our TikTok! Be sure to subscribe to our YouTube... -
Help Us Name Blugene’s New Virus Friend
TypeBlog PostPublishedJan. 7, 2020, 1:55 p.m....your favorite name. We hope you submit your best ideas because the finalists will receive some newly designed...Learn more about Blugene's make-over Get a how-to guide on the new Addgene search engine ... -
Introducing the Addgene Antibodies 101 eBook!
TypeBlog PostPublishedOct. 29, 2024, 1:15 p.m....Sorting” is there to help guide you through the process. If you’re confident in your application and protocols... -
Lucky Thirteen: Guest Contributors of 2023
TypeBlog PostPublishedDec. 5, 2023, 2:15 p.m...., tools, and knowledge. Guest authors The Codon Guide to Synthetic BiologyAuthor: Niko McCartyAntibodies...globe find and share knowledge, resources, and new ideas - we're so grateful for your writing, your knowledge... -
We’re Going Viral This Week!
TypeBlog PostPublishedOct. 14, 2019, 1 p.m....Learn more about the Data Hub in this blog post. Alongside our efforts to facilitate the sharing of reagents...Learn more about the viral service Read our AAV guide ... -
Twenty Years of Sharing Science!
TypeBlog PostPublishedJan. 8, 2024, 3:25 p.m....experience we're sharing through blogs, eBooks, videos, guides, protocols, and of course, the friendly folks... -
Genome Engineering, CRISPR, and Research in Singapore: Interview with Wei Leong Chew
TypeBlog PostPublishedFeb. 6, 2018, 2 p.m....primary investigator. Coming up with new project ideas, applying for grants, and mentoring. Chapter 5: ...Find Synthetic Biology Plasmids Browse our CRISPR Guide Pages ... -
Addgene’s GIPHY Page is Here to Help You Express Yourself (and Your Gene of Interest)
TypeBlog PostPublishedAug. 17, 2021, 1:15 p.m....informative and entertaining protocol and explainer videos for you to level up in the lab, and we’ve started... could be you after watching Addgene’s protocol videos. via GIPHY If you’re also looking for that... -
Twelve Amazing Guest Blog Contributors!
TypeBlog PostPublishedNov. 26, 2024, 2:15 p.m.... from IPI Simon Moe, from Promega, who wrote a guide to selecting fluorescent dyes and ligands. Illuminating...Controls Priyamvada Prathima, who wrote a helpful guide to understanding your flow data. Flow Cytometry... -
Celebrating the 2024 Nobel Prize in Chemistry
TypeBlog PostPublishedOct. 17, 2024, 1:15 p.m....awarded the Nobel Prize in Chemistry last week alongside Demis Hassabis and John Jumper! The Baker Lab ...like high resolution Ras sensors and crosslinked peptides proposed as a scaffold for a new generation of... -
Antibody Data Repositories and Search Engines
TypeBlog PostPublishedOct. 31, 2024, 1:15 p.m....share validation and experimental data to help you decide if the antibody is a good fit for your experiment...improving antibody quality throughout research, consider checking out the Only Good Antibodies community...