We narrowed to 791 results for: Kin
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TypeBlog PostPublishedApril 26, 2022, 1:15 p.m....relative quantification - that is, since you’re looking at the amount of DNA in your sample relative to...
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When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog PostPublishedApril 19, 2016, 2:30 p.m.... experiments. Considerable effort has gone into making FPs monomeric, yet many investigators remain ignorant... -
Anatomy of a Plasmid Page at Addgene
TypeBlog PostPublishedFeb. 4, 2016, 3:30 p.m....found yourself bamboozled by all of the different kinds of information on our plasmid pages? Well, to help... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog PostPublishedSept. 28, 2017, 2:01 p.m....Multi-color labeling of neurons is for more than making pretty pictures (see figure 4). It also enables... -
Great Results Start with Great Standard Curves
TypeBlog PostPublishedAug. 15, 2024, 1:15 p.m....complex trendlines. If you don’t have access to this kind of software, don’t give up! Rather than plotting... -
Tips for arabidopsis transformation
TypeBlog PostPublishedOct. 25, 2018, 1:23 p.m....phenotypic analysis it is appropriate to begin working in the T2 generation. The selection process itself... -
An Introduction to Adenovirus
TypeBlog PostPublishedOct. 3, 2023, 1:15 p.m....resources References Bauzon, M., Castro, D., Karr, M., Hawkins, L. K., & Hermiston, T. W. (2003). Multigene expression... -
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons
TypeBlog PostPublishedDec. 7, 2020, 2:53 p.m....array. Workflow and technical considerations Making insertions in bacterial genomes with the V. cholera... -
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries
TypeBlog PostPublishedOct. 4, 2018, 12:44 p.m.... Addgene Blog Read this blog post about CRISPR Kinome Libraries available at Addgene Learn about the ... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog PostPublishedMarch 17, 2016, 2:30 p.m....been limited, in part, due to the necessity for working with this virus under the highest biosafety level... -
How to Write a Scientific Review Article
TypeBlog PostPublishedFeb. 16, 2017, 3:30 p.m....So, as long as it doesn't look done, just keep working on it. Also bear in mind that this is just a review... -
Hot Plasmids: Fall 2024
TypeBlog PostPublishedNov. 21, 2024, 1:30 p.m....assembly of the split luciferase is reversible, making PILATeS suitable for live imaging with a time resolution... -
The PAM Requirement and Expanding CRISPR Beyond SpCas9
TypeBlog PostPublishedAug. 20, 2020, 2:30 p.m....Cas13 enzymes require a single base protospacer flanking sequence (PFS) adjacent to the target, but many... -
Four Base Editing Reporters to Monitor and Enrich Editing in Real-time
TypeBlog PostPublishedJuly 7, 2020, 1:15 p.m....APOBEC-Cas9 editosome would restore eGFP fluorescence, making it easy to quantify single base editing efficiency... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog PostPublishedJuly 2, 2019, 12:51 p.m....neuron-specific promoters, including calmodulin-dependent kinase II alpha (aCaMKII; excitatory neurons) and Synapsin... -
CRISPR-mediated Plant Base Editors
TypeBlog PostPublishedJan. 3, 2019, 1:35 p.m.... repaired by cellular mismatch repair machinery making an original C-G pair converted to T-A or reverted... -
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
TypeBlog PostPublishedNov. 1, 2018, 12:35 p.m....through a second recombination event between the two flanking repeats. In cells where recombination has not ... -
Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research
TypeBlog PostPublishedJuly 29, 2025, 1:15 p.m.... version of TnpB is just 408 amino acids long — making it one of the most compact genome editors to date... -
QC Sequencing Technologies at Addgene
TypeBlog PostPublishedJuly 15, 2025, 1:15 p.m....choose the best method based on the plasmid. By working with the Data team, the QC team has also improved... -
Plasmids 101: Control Plasmids
TypeBlog PostPublishedApril 9, 2015, 3:29 p.m....results can be compared; results from an experiment lacking the proper controls are invariably inconclusive...