We narrowed to 813 results for: TIM

1. Educational Planarian Tools for Teaching Developmental Biology Techniques

   Type

   Blog Post

   Published

   May 24, 2018, 1:20 p.m.

   ... for viewing how gene expression changes through time or after genetic manipulation. The technique involves...

2. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

   Type

   Blog Post

   Published

   May 1, 2018, 1:57 p.m.

   ... interact with the bait at a particular point in time, i.e. when the bait is interacting with a particular...

3. Viral Vectors 101: Calcium Sensors

   Type

   Blog Post

   Published

   Aug. 10, 2023, 1 p.m.

   ...in vivo studies. There are different versions optimized for neuron-specific, astrocyte-specific (GCaMP6...

4. Viral Vectors 101: Virus Safety

   Type

   Blog Post

   Published

   June 22, 2023, 1:15 p.m.

   ...1. In BSL-2 labs, eye protection is required any time you have to work with BSL-2 materials, such as lentivirus...

5. Antibodies 101: Choosing the Right Isotype

   Type

   Blog Post

   Published

   April 6, 2023, 3 p.m.

   ...greater amount of antigen in a shorter period of time and would likely perform better for probing poorly...

6. Hot Plasmids and Viral Preps - March 2021

   Type

   Blog Post

   Published

   March 23, 2021, 1:15 p.m.

   ...this TIR1 complex binds to the degron sequence ultimately leading to the degradation of the AID-tagged ...

7. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

   Type

   Blog Post

   Published

   May 7, 2020, 1:15 p.m.

   ...cells. While editing single genes is very useful, sometimes, you need to modify multiple genes to generate...

8. Synthetic promoter AAVs for cell-type specific expression in retinal cells

   Type

   Blog Post

   Published

   March 12, 2020, 1:15 p.m.

   ...genes in particular tissues, but they are costly and time-consuming to generate, especially for large animals...

9. Mouse Modeling, Part 1: Genetically Engineered Mice

   Type

   Blog Post

   Published

   July 11, 2019, 1:26 p.m.

   ...the transgene in specific cells or under certain stimuli. Cre recombinase catalyzes the recombination of...

10. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type

    Blog Post

    Published

    Oct. 22, 2019, 12:20 p.m.

    ...not be physically present at your poster at all times, so other participants can identify you. You may...

11. Hot Plasmids and Viral Preps - January 2021

    Type

    Blog Post

    Published

    Jan. 5, 2021, 2:15 p.m.

    ...experiments that require high photostability, such as time-lapse imaging or with high light power detection...

12. Progress Towards a PAM-Free CRISPR

    Type

    Blog Post

    Published

    March 7, 2024, 2:39 p.m.

    ...desired edit to still occur if the cut site is sub-optimal? As with everything in biology, the answer is always...

13. Hot Plasmids February 2024

    Type

    Blog Post

    Published

    Feb. 20, 2024, 2:45 p.m.

    ... The Junwei Shi Lab addressed this problem by optimizing the Cas protein delivery with Peptide-Assisted...

14. Plasmids 101: FLEx Vectors

    Type

    Blog Post

    Published

    April 28, 2016, 2:30 p.m.

    ...allowing for rescued expression of GeneR.   Sometimes also referred to as DiO (Double-floxed inverse...

15. Plasmids 101: Terminators and PolyA signals

    Type

    Blog Post

    Published

    March 31, 2016, 2:30 p.m.

    ...contributes to variability in RNA half-life, and ultimately gene-expression. Polyadenylation is the post-...

16. Truncated gRNAs for Regulating Gene Expression

    Type

    Blog Post

    Published

    Jan. 10, 2017, 3:37 p.m.

    ...protospacer. The resulting double strand break (DSB) stimulates DNA repair mechanisms that can be exploited to...

17. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type

    Blog Post

    Published

    March 28, 2018, 6:52 p.m.

    ...so far! Why do we need PAM flexibility? IDT estimates that there is an NGG PAM every 42 bases in the...

18. Degrading DNA with Cascade-Cas3

    Type

    Blog Post

    Published

    Feb. 11, 2025, 2:15 p.m.

    ...of course, only hypothetical at this point. More time and energy must be devoted to studying Cas3 and ...

19. The AAVantages of AAV in CRISPR Screens

    Type

    Blog Post

    Published

    Feb. 4, 2025, 2:15 p.m.

    ...expressed in non-dividing cells for long periods of time. This reduces the chance of integration at random...

20. Antibodies 101: Flow Compensation

    Type

    Blog Post

    Published

    May 16, 2024, 1:15 p.m.

    ...before moving onto your experimental samples. With time and practice, you may be able to do your compensation...