We narrowed to 816 results for: ache

1. Troubleshooting Your Plasmid Cloning Experiment

   Type

   Blog Post

   Published

   Sept. 24, 2019, 12:55 p.m.

   ...these steps, you might want to reconsider your approach, and try a different cloning strategy. Once you've...

2. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

   Type

   Blog Post

   Published

   Sept. 4, 2018, 12:53 p.m.

   ...arbitrary DNA sequences and have been shown to achieve ~100 fold repression in E. coli. In addition, TALEs...

3. RaPID Detection of RNA-protein Interactions

   Type

   Blog Post

   Published

   Aug. 16, 2018, 12:40 p.m.

   ...work. APEX2, a faster peroxidase-based labeling approach, is an alternative option but it’s toxic to tissues...

4. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

   Type

   Blog Post

   Published

   Jan. 31, 2019, 1:32 p.m.

   ...on the extracellular matrix and fibroblasts. With each cell mechanism discovered in microenvironments, ...

5. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

   Type

   Blog Post

   Published

   Aug. 5, 2021, 12:15 p.m.

   ...construction of the dual virus and the experimental approach. (B) CR-Cre mice demonstrate pain response due...

6. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

   Type

   Blog Post

   Published

   May 11, 2021, 1:15 p.m.

   ... making fluorescent protein fusions, check out Joachim Goedhart’s article on the Addgene blog: Fluorescent...

7. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

   Type

   Blog Post

   Published

   April 13, 2017, 2:30 p.m.

   ... assays Long-lived protein degradation: This approach measures the degradation rate of radio-labeled...

8. Developing Lab Management Software for Biology

   Type

   Blog Post

   Published

   Aug. 25, 2016, 2:30 p.m.

   ...Use tried-and-true tools We use Python, Django, Apache, and MariaDB. Using tried-and-true tools means ...

9. Gendered Innovations: Why Does Sex of the Cell Matter?

   Type

   Blog Post

   Published

   July 21, 2016, 2:30 p.m.

   ...and Western Europe have taken three strategic approaches to gender equality over the past several decades...

10. Plasmids 101: Golden Gate Cloning

    Type

    Blog Post

    Published

    Aug. 27, 2015, 2:30 p.m.

    ...variety of restriction enzyme-based cloning methods. Each method has its own pluses and minuses, but Golden...

11. Plasmids 101: Cre-lox

    Type

    Blog Post

    Published

    Jan. 13, 2015, 3:47 p.m.

    ...double-strand DNA break occurs within the core spacer of each loxP site and the two strands are ligated, resulting...

12. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type

    Blog Post

    Published

    Nov. 7, 2017, 1:59 p.m.

    ...back into the infants. Almost all patients have achieved persistent immunological reconstitution with a...

13. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type

    Blog Post

    Published

    July 23, 2020, 1:20 p.m.

    ...inducible promoters, light, or small molecules could achieve rapid, dynamic, and spatial control of CRISPR-Cas...

14. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type

    Blog Post

    Published

    June 25, 2020, 1:15 p.m.

    ...has helped me realize that is not true at all. Racheal Komuhendo, a Lab Technician, dreamed of becoming...

15. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type

    Blog Post

    Published

    Oct. 22, 2019, 12:20 p.m.

    ...choose essentially any background, but two common approaches are either a solid color, or a “themed” background...

16. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type

    Blog Post

    Published

    Sept. 26, 2019, 12:50 p.m.

    ...ranging from 11% to 100%, a level similar to other approaches.  Nanoblades can also be used to non-invasively...

17. Plasmids 101: The Wide World of Natural Plasmids

    Type

    Blog Post

    Published

    Oct. 8, 2024, 1:15 p.m.

    ...genes that help bacteria invade hosts, including attaching to host cells and evading the host immune system...

18. Overcoming the Challenges of Lentiviral Production

    Type

    Blog Post

    Published

    Aug. 7, 2018, 12:31 p.m.

    ...example, in our hands we find that a double harvest approach at 48 and 72h produces high titer virus for transfer...

19. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type

    Blog Post

    Published

    June 26, 2018, 1:33 p.m.

    ..., Michael McMurray, and Richard Gronostajski who each are being awarded free plasmids form Addgene to ...

20. Corralling and Cornering the Goldilocks Antibody

    Type

    Blog Post

    Published

    Aug. 9, 2022, 1:15 p.m.

    ...exposure times. To test the antibody’s reproducibility, each antibody is independently screened twice, confirming...