We narrowed to 804 results for: arc

1. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

   Type

   Blog Post

   Published

   Dec. 10, 2020, 1 p.m.

   ...using adenoviral vectors with your CRISPR/Cas9 research! To find more information about the adenoviral...

2. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

   Type

   Blog Post

   Published

   April 4, 2019, 12:53 p.m.

   ...knockdown, however, keeps bacteria viable and allows researchers to study if that gene is a target of an antibiotic...

3. Viral Vectors 101: Voltage Indicators

   Type

   Blog Post

   Published

   Jan. 30, 2024, 2:15 p.m.

   ...ability to monitor their signals – electricity! The research tools for studying voltage within a cell are genetically...

4. Tips for a 1st time AAV user (by a Rookie AAV user)

   Type

   Blog Post

   Published

   Oct. 23, 2018, 11:49 a.m.

   ...out Addgene's virus protocols Find AAV for your research ...

5. Fluorescent Tagging of Endogenous Genes with SapTrap

   Type

   Blog Post

   Published

   Oct. 12, 2017, 2:26 p.m.

   ...with either blasticidin or puromycin selection. Researchers at the Allen Institute for Cell Science recently...

6. Cloning Mammalian Cells with the Agarose Method

   Type

   Blog Post

   Published

   Sept. 7, 2017, 12:17 p.m.

   ... biological screens. For example, for cancer researchers, if growth properties in agarose are of interest...

7. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

   Type

   Blog Post

   Published

   June 22, 2017, 2:30 p.m.

   ...function of a protein or its role in a disease, researchers often isolate proteins of interest and examine...

8. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

   Type

   Blog Post

   Published

   May 22, 2015, 4:58 p.m.

   ...exclusively because historical studies of pathogen research have conclusively shown that barrier protocols...

9. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

   Type

   Blog Post

   Published

   Aug. 30, 2022, 1:15 p.m.

   ...your target epitope(s). To achieve these goals, researchers commonly use one of two types of fixatives - ...

10. DNA Purification Without a Kit

    Type

    Blog Post

    Published

    Feb. 11, 2020, 2:15 p.m.

    ...number of spin columns you need to complete your research. Do you know of other nucleic acid purification...

11. A Guide to Starting Your Own Journal Club

    Type

    Blog Post

    Published

    Feb. 6, 2020, 3 p.m.

    ... of learning about new cutting edge science, researchers of all varieties must stay current with literature...

12. Antibodies 101: Producing Recombinant Antibodies

    Type

    Blog Post

    Published

    Aug. 13, 2024, 1:15 p.m.

    .... Recombinant Antibodies in Basic Neuroscience Research. Curr Protoc Neurosci. 2020 Dec;94(1):e106. doi...

13. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type

    Blog Post

    Published

    Oct. 10, 2023, 1 p.m.

    ...universally compatible with all bacteria used in research, it isn’t a widely popular selection agent. Pros...

14. Plasmids 101: Secondary Nanobody Toolbox

    Type

    Blog Post

    Published

    Feb. 27, 2018, 2:04 p.m.

    ...you ready to start using nanobodies for your own research? Great! Pleiner et al. have done the hard work...

15. Tips for Screening with Yeast Two Hybrid Systems

    Type

    Blog Post

    Published

    Oct. 22, 2015, 2:30 p.m.

    ...that retain their individual activities (1). Researchers also discovered the modular format of some transcriptional...

16. Cpf1: A New Tool for CRISPR Genome Editing

    Type

    Blog Post

    Published

    Oct. 14, 2015, 2:30 p.m.

    ...location and GC-content. After sequencing and searching for cellular RNAs important for Cpf1 function,...

17. Plasmids 101: Protein tags

    Type

    Blog Post

    Published

    Dec. 11, 2014, 4:26 p.m.

    ...decades of trying to address these challenges, researchers have amassed a considerable molecular tool box...

18. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type

    Blog Post

    Published

    Oct. 13, 2022, 1:45 p.m.

    ...modifications. Cas9 has been modified to enable researchers to knock out, knock in, base edit, activate, ...

19. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type

    Blog Post

    Published

    Sept. 16, 2020, 1 p.m.

    ... efficiency than its wild-type counterpart.  In search of a smaller, but equally potent Cas9 protein, ...

20. Finding nucleic acids with SHERLOCK and DETECTR

    Type

    Blog Post

    Published

    April 16, 2020, 1 p.m.

    ...Durbin AF, Kellner MJ, Tan AL, Paul LM, Parham LA, Garcia KF, Barnes KG, Chak B, Mondini A, Nogueira ML, ...