We narrowed to 884 results for: ica;

...results. Most cell culture media contain the pH indicator phenol red (pH range 6.0 (yellow) - 8.0 (red))...Anything between orange and light orange usually indicates that the cells have grown enough, are making their...analysis of bisphenol A-induced perturbation in biological processes in ER-negative HEK293 cells." PLoS ...

...:  my undergrads were different people! More specifically, they had different learning styles. One was...delegating well, but it does get easier over time! Communicate often and clearly As a manager you need to clearly...it is the best way to avoid mistakes due to miscommunication and to detect trouble. I’m not comfortable...

...their fused protein under certain conditions (typically the addition of an exogenous small molecule). ...in size from several kDa up to ~35 kDa and are typically knocked-in via gene targeting or cloned in-frame... F-box protein must be expressed (inducibly, ectopically, stably… it’s your call). The TIR1 F-box protein...

...however, your cost per cloning reaction drops dramatically, as one tube of enzyme should be enough for ...there is no need for restriction digests, gel purifications, or ligations. The general Gateway cloning protocol...Chaokun, et al. "FastCloning: a highly simplified, purification-free, sequence-and ligation-independent PCR ...

... to synthesize a large volume of information: basically, a scientist’s tool kit. I wrote a review article... was cheating. Eventually I learned that it’s practical because there simply isn’t enough time to read...used both. Curate and present some useful data Typically lists and pictures are the most useful parts of...

...carry different antibiotic resistance markers (indicated here by plasmid color), allowing you to easily...is simple -  no restriction, ligation or gel purification steps are required! Multiple fragment cloning...the attB, P, L and R sites that recombine very specifically and directionally: attB1 sites react only with...

... will have numbers by gated populations; this indicates the percentage of total events contained in the... gated population. Heat maps are often used to indicate the relative density of cells. You can see both...presented. A horizontal line across the peak will indicate the cut-off value for a positive signal (Figure...

... use is pX333 from the Ventura lab. pX333, a modification of pX330, contains humanized wtCas9 and two ...thus marking cells that have undergone genomic modification. In this system, Cas9 must be supplied on a ... combinatorial mammalian genetics.” Journal of Clinical Investigation 125(4) (2015):1603-19. PubMed PMID...

... suggestions in the comments.  X has moved significantly away from the functionality and features that...this reason, our timeline has accelerated. We significantly reduced posting on November 20, 2023, and as...

... showed that a laughter program significantly decreased immunological markers of stress in adolescents...

...Finally, our team is rounded out with our science communication specialist (you'll know me by my Tweeting — ...leading a professional development workshop. Specifically, Jo, Brook, and Angela are co-leading "Making...

...software engineers, surprisingly, who have deep technical knowledge but little biology experience.) The ...synthetic biology, DNA assembly, and various applications. I think these papers are good starting points...

...open platform for scientists using AAV to share practical information about their experiences. It will contain...important details that you won’t find in the final publication and we have already collected over 100 entries...

...Rob Hurt, whose expertise and suggestions were critical to the development and writing of two blog posts...Modular Cloning and Plasmids 101: Modular Cloning Applications and Kits We are very grateful for each and...

...Sapozhnikov tells us how to use dCas9 (with no modifications!) as a CRISPR/dCas9-based tool for DNA demethylation...demethylation. AAV Q&A with Tim Miles: While not technically a research story, I couldn't resist adding in...

...Videos page! Videos are a powerful tool for communicating scientific knowledge. On this new page, we have...  Our first video, featuring Outreach Scientist Jessica Welch, can be found below: How-to videos Our...

...Addgene will be at the American Society for Microbiology Microbe meeting here in Boston next week - be... Jessica Welch...

... specificities, from target-specific ones to application-specific ones to anything goes. We've shared ...repositories and search engines Website Targets Application Purpose Notes Antibodypedia Any Any...

...lighting/HVAC requires a significant energy expenditure here at Addgene. These are critical systems that cannot...

...produce either a gRNA, a nuclease, or both. Applications of CRISPR: Cut: CRISPR can be used to cut the...all sequences. However, prime editing and the identification of other Cas proteins continue to expand the...