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We narrowed to 874 results for: sta

Showing: 761 - 780 of 874 results

  1. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...CNS and PNS! What is the CREATE method?  CREATE stands for Cre Recombinase-based AAV Targeted Evolution...number of neurons overall (GFP+ cells that also stained for a neuronal marker). Check out tables 1 and ...

  2. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ... (MCS). Modules are assembled one at a time by standard restriction enzyme digestion followed by ligation...Goler JA, Arkin AP, et al. BglBricks: A flexible standard for biological part assembly. Journal of biological...

  3. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    Published
    March 20, 2014, 3:04 p.m.
    ... should not even be the real goal. We are all constantly negotiating our energy and attention. The goal...going away to a conference. Really. No one will starve while you are away a few days. Also, your children...

  5. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...fluorescently labeled piggyBac vectors for uniform and sustained expression of single or multiplexed gRNAs and ...includes a CRISPRi-based synthetic oscillator, bistable network, stripe pattern-forming incoherent feed-forward...

  6. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...Tong, Wang, et al., 2023). Based on this promising start, the team used rational mutagenesis to produce the...narrowed the editing window and reduced A → G bystander edits. Their final tool, ACBE-Q (for an N108Q ...

  7. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ... T’s. Thus, it’s important that there are no “bystander bases” if you choose this system.  Fig....demonstrated that targeting dCas9 to transcriptional start sites was sufficient to repress transcription by...

  8. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...tool for sharing ideas. This leads to everyday obstacles for many of us, such as finding time to read all...platforms (such as social networking, visual appeal, instant sharing across multiple other sites), I created...

  9. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...prevent your gene from being up-regulated. For instance, generally, the more highly expressed a gene is...whichever guides are closest to the transcription start site (TSS). We recommend targeting the guides to...

  11. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...yourself up for a screening success? Before you start analyzing individual clones, it’s wise to quickly...can ensure you aren’t looking for a needle in a haystack before going on to generate and screen individual...

  12. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ... to lab. Promega supports the Addgene mission statement: Accelerate research and discovery by improving...evolution of the coelenterzine substrate to the more stable yet brighter furimazine by Promega’s Chemistry ...

  13. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ... had the lowest background activity in the dark state and highest fold induction at 31X. By using a slit...NPPOC-caged trimethoprim (TMP) Cas9 fused to destabilizing domains (Cas9-DHFR) Gene editing, transcription...

  14. New Coomassie Protocol Video

    Type
    Blog Post
    Published
    Oct. 31, 2023, 1:15 p.m.
    ...out our latest protocol video, "Coomassie Purity Stain of Recombinant Antibodies."  Hot tip! This protocol...

  15. New Antibodies in the Lab Animation!

    Type
    Blog Post
    Published
    Nov. 19, 2024, 2:15 p.m.
    ...applications in your experiment...but not sure where to start or what your options are? Addgene's latest animation...

  16. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    Published
    Sept. 5, 2023, 1:15 p.m.
    ...they go to Addgene.”-Ryan Mehl, Professor, Oregon State University. ...

  17. Addgene Launches Partnership with IPI

    Type
    Blog Post
    Published
    June 13, 2023, 1:15 p.m.
    ...antibody protocols, and Antibodies 101 blog series.  Stay tuned for more information on our blog and social...

  18. Antibodies 101 Series on TikTok

    Type
    Blog Post
    Published
    March 19, 2024, 1:15 p.m.
    ...our videos will also be shared on our @addgene Instagram account, or you can watch the full animation on...

  20. 2022 Depositor Week

    Type
    Blog Post
    Published
    Dec. 12, 2022, 2:15 p.m.
    ...  Monday: Thank you post (this is it!)  Tuesday: Stats roundup on Twitter Wednesday: 2022 Blue Flame Depositors...
Showing: 761 - 780 of 874 results