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TypeBlog PostPublishedMarch 20, 2014, 3:04 p.m.... should not even be the real goal. We are all constantly negotiating our energy and attention. The goal...going away to a conference. Really. No one will starve while you are away a few days. Also, your children...
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CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?
TypeBlog PostPublishedOct. 13, 2022, 1:45 p.m.... T’s. Thus, it’s important that there are no “bystander bases” if you choose this system. Fig....demonstrated that targeting dCas9 to transcriptional start sites was sufficient to repress transcription by... -
CRISPR 101: Any Base Transversion Editors
TypeBlog PostPublishedApril 1, 2025, 1:15 p.m....Tong, Wang, et al., 2023). Based on this promising start, the team used rational mutagenesis to produce the...narrowed the editing window and reduced A → G bystander edits. Their final tool, ACBE-Q (for an N108Q ... -
Using Video to Share Your Science: We Share Science
TypeBlog PostPublishedNov. 3, 2016, 2:30 p.m....tool for sharing ideas. This leads to everyday obstacles for many of us, such as finding time to read all...platforms (such as social networking, visual appeal, instant sharing across multiple other sites), I created... -
Hot Plasmids - August 2020
TypeBlog PostPublishedAug. 18, 2020, 1:15 p.m....fluorescently labeled piggyBac vectors for uniform and sustained expression of single or multiplexed gRNAs and ...includes a CRISPRi-based synthetic oscillator, bistable network, stripe pattern-forming incoherent feed-forward... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog PostPublishedJuly 23, 2019, 12:46 p.m....role as a receptor for PHP.B. The Deverman lab started by seeing how changes in LY6A’s expression affected... References Holmes, Christina, and William L. Stanford. "Concise review: stem cell antigen‐1: expression... -
CRISPR Activation: A Practical Guide
TypeBlog PostPublishedAug. 18, 2016, 2:30 p.m....prevent your gene from being up-regulated. For instance, generally, the more highly expressed a gene is...whichever guides are closest to the transcription start site (TSS). We recommend targeting the guides to... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog PostPublishedSept. 1, 2020, 1:15 p.m....split vectors has been inconsistent. A greater understanding of basic AAV biology, however, would help improve...and effective in the retina of a mouse model of Stargardt disease. Hum Mol Genet 24:6811–6825 . https://... -
CRISPR 101: Validating Your Genome Edit
TypeBlog PostPublishedNov. 3, 2022, 12:15 p.m....yourself up for a screening success? Before you start analyzing individual clones, it’s wise to quickly...can ensure you aren’t looking for a needle in a haystack before going on to generate and screen individual... -
Technologies Enabled by NanoLuc® Luciferase
TypeBlog PostPublishedFeb. 8, 2018, 12:17 p.m.... to lab. Promega supports the Addgene mission statement: Accelerate research and discovery by improving...evolution of the coelenterzine substrate to the more stable yet brighter furimazine by Promega’s Chemistry ... -
Optogenetics + CRISPR, Using Light to Control Genome Editing
TypeBlog PostPublishedSept. 3, 2020, 12:15 p.m.... had the lowest background activity in the dark state and highest fold induction at 31X. By using a slit...NPPOC-caged trimethoprim (TMP) Cas9 fused to destabilizing domains (Cas9-DHFR) Gene editing, transcription... -
New Coomassie Protocol Video
TypeBlog PostPublishedOct. 31, 2023, 1:15 p.m....out our latest protocol video, "Coomassie Purity Stain of Recombinant Antibodies." Hot tip! This protocol... -
New Antibodies in the Lab Animation!
TypeBlog PostPublishedNov. 19, 2024, 2:15 p.m....applications in your experiment...but not sure where to start or what your options are? Addgene's latest animation... -
Plasmids 101 eBook 4th Edition
TypeBlog PostPublishedSept. 5, 2023, 1:15 p.m....they go to Addgene.”-Ryan Mehl, Professor, Oregon State University. ... -
Addgene Launches Partnership with IPI
TypeBlog PostPublishedJune 13, 2023, 1:15 p.m....antibody protocols, and Antibodies 101 blog series. Stay tuned for more information on our blog and social... -
Antibodies 101 Series on TikTok
TypeBlog PostPublishedMarch 19, 2024, 1:15 p.m....our videos will also be shared on our @addgene Instagram account, or you can watch the full animation on... -
#DeckTheLab to Win a Tasty Winter Treat This Holiday Season
TypeBlog PostPublishedNov. 30, 2015, 3:30 p.m....submissions at random and deliver the prizes at the start of the New Year. Will your lab be a lucky winner... -
'Tis the Season to #DeckTheLab
TypeBlog PostPublishedDec. 2, 2016, 3:30 p.m....winning submissions and deliver the prizes at the start of the New Year. Will your lab be a lucky winner... -
Help Us Name Blugene’s New Virus Friend
TypeBlog PostPublishedJan. 7, 2020, 1:55 p.m....UPDATE: The contest is now closed. Stay tuned to see the finalists and vote for your favorite! Our mascot... -
2022 Depositor Week
TypeBlog PostPublishedDec. 12, 2022, 2:15 p.m.... Monday: Thank you post (this is it!) Tuesday: Stats roundup on Twitter Wednesday: 2022 Blue Flame Depositors...