...transcriptional activators: some methods rely on fusion proteins while others re-engineer components of...Description CRISPR activation can occur through fusing dCas9 with VP64, a strong transcriptional activation...transcription. This is done through creating a dCas9/VP64 fusion protein engineered with aptamers that bind to MS2...dCas9, SunTag uses a repeating peptide array to fused with multiple copies of VP64. By having multiple...consistently throughout cells. VPR Description VPR fuses a tripartite complex with dCas9 to activate transcription...notable CRISPR activators is that it requires a fusion protein, rather than relying on a two-component... activation. dCas9-CBP In this method, dCas9 is fused to CBP, an histone acetyltransferase domain capable...

...by splitting Cre and fusing each half with PhyB or PIF6, phytochrome-like fusion proteins from the plant...unmodified enzyme. Each half of the base editor is fused to a fast-splicing split intein and co-infected ...plasmids containing a red-shifted Ace-mScarlet, a fusion of a voltage-sensitive inhibitory rhodopsin (Ace2N...mScarlet emission. Figure 2: Ace-mScarlet is a fusion between the voltage-sensitive inhibitory rhodopsin...soon as red light or PCB is no longer present, the fusion proteins separate, inactivating Cre. This is critical...Cre (CreC) and the N-terminus of Cre (CreN) are fused to the truncated Phytochrome B (PhyBΔ) and the active...

...ternary complex formation between the FKBP12F36V-fusion and the ubiquitin proteasome machinery through ...expression of N- or C-terminally FKBP12F36V-tagged fusion chimeras or locus-specific knock-in of FKBP12F36V...running in your lab Confirm the functionality of your fusion chimeras For each protein-of-interest, it is important...of comparing functionality of N- and C-terminal fusions and degradation in a cell line of interest prior... used to evaluate degradation of the FKBP12F36V-fusion chimera of interest. In the cell lines we have ...provide the best confirmation of selective FKBP12F36V fusion chimera degradation. In the cell lines we have ..., Erb et al., Weintraub et al.), assessments of fusion chimera functionality (Nabet et al., Erb et al....

...reversible oligomerization when fused to a protein of interest. Fusion protein oligomerization happens...cryptochrome-interacting basic-helix-loop-helix) protein. After fusing the CRY2 and CIB1 domains to separate proteins ...used to control homo-oligomerization of a protein fused to CRY2, although some studies have shown that this...derivative of the opsin CoChR, termied soCoChR, by fusing a fragment of the kainate receptor subunit to the... the C-terminus of the protein. Unlike the diffuse neuronal expression observed with wild type CoChR, ...which TagRFP-T and Dronpa flanked a PKA substrate fused to the phosphopeptide binding domain FHA. The FHA1...

...repressor) fusion protein, the authors show that the VP16 transcription activation domain fusion increases... in cellular functional imaging and in protein fusions. The Gadella lab has deposited all mScarlet constructs...activity. In addition, proteins can be “caged” by fusion to specific domains thereby rendering them inactive... base editing technique uses dCas9/Cas9 nickase fused to a cytidine deaminase for targeted conversion ...minimum efficiency of ~50% and confirmed that the fusion protein retained the PAM properties of the individual...the B. subtilis libraries Transcription factor fusions elucidate iPSC reprogramming mechanism The Ptashne... efficiency of iPSC reprogramming while the HP1 fusion eliminates reprogramming. These results argue that...

...validate that the fusion protein localizes properly by comparing the signal from the fusion itself to the ...fluorescent tag. Once expressed as a fluorescent protein fusion, it is possible to track your protein of interest...finally degraded (2, 3). Once you've created your fusion protein, it's important to validate its function...always possible, to use inert fluorescent protein fusions to visualize subcellular structures. For example...example, you might use your fluoresent protein fused to a signal sequence that traffics the fluorescent protein...size – it is possible that a large or charged FP fusion can change the subcellular localization of a protein...

...are just one (admittedly large) class of common fusion protein tags. Biochemists and molecular biologists...amassed a considerable molecular tool box of tags and fusion proteins to aid in the expression and purification..., glutathione S-transferase (GST), which can be fused to recombinant proteins for one-step purification...immunoprecipitating, or purifying that protein may be to fuse an affinity tag to it. The FLAG, hemaglutinin antigen...column. Like other affinity tags, a His tag can be fused to either the N- or C-terminus of a protein. Unlike... available, and though adding too many tags and fusion proteins to your protein of interest would eventually...scientists use tags, with a few time-tested tags and fusion proteins as examples. The tables list more common...

...vacuolar/lysosomal and cytoplasmic Rosella. When fused with a mitochondria-targeting sequence, Rosella ...mitochondria, or mitophagy. Rosella can also be fused to a protein of interest to track the autophagy ... This autophagy biosensor contains mRFP and GFP fused to the autophagy-related microtubule-associated ...puncta turning red (GFP negative/RFP positive) upon fusion of an autophagosome with a lysosome. An increase...to more yellow and red puncta, while a block at fusion of autophagosomes with lysosomes leads to an increase...Fluorescent Protein, but following translation, this fusion protein is cleaved to generate one molecule of ...

...Nano-lanterns contain a Renilla luciferase variant fused to an FP; when supplied with a luciferase substrate...applications, including optogenetics, biosensors, and fusion proteins. Solving the problems of low luminescence...Saito et al. described the first Nano-lantern, a fusion of a mutagenized Renilla luciferase (RLuc8) with...Renilla luciferase variant are used to excite the fused fluorescent protein. This technique is very similar...compartments over several minutes using Nano-lantern fusion proteins. To further improve long-term imaging,... as good reporter genes for endogenous protein fusions created using CRISPR/Cas9. eNLs offer high luminescent...

...Monitor dynamics of vesicle fusion and release from the plasma membrane by fusing various vesicle cargoes ...more popular timers (whether as part of protein fusions or expressed independently) originally fluoresce...expressed, either in whole cells or as a pool of fusion proteins localized to a specific region of the ...expressing a single FP-timer protein or protein fusion. This one protein method simplifies cloning and...between models for trafficking to the lysosome by fusing Medium-FT to LAMP-2A, a lysosome-associated membrane...