...nucleus to cell membrane, this collection has fluorescently tagged versions of many proteins known to localize...spectra. A comprehensive list of cell markers all tagged with mEmerald. Plasmids to monitor protein-protein...available targets since the Davidson lab always tagged new proteins with mEmerald first, Baird says. From...story about his life’s work and the stage IV lung cancer that has now prompted him to deposit the tools...Michael Davidson’s lab at Florida State University has been the go-to place. In 2012, his National High ...backbones alone,” said Addgene’s Lianna Swanson, who has been working with members of Davidson's lab to coordinate...coordinate the impressively big deposit. “He has every fluorescent protein under the sun, from the standard...

...gene expression patterns. The lab has also used CellTags for tagging two cell populations in a competitive...the single-cell level with CellTag CellTagging is a lentiviral based tagging method that labels cells with...Each cell is labeled with 3-4 tags to increase the diversity of the CellTag library. The fixed region acts...library-specific identifier and allows cells to be tagged with different CellTag libraries at multiple timepoints during...inefficient. Cells were sequentially tagged with different CellTag libraries at three time points during...reprogramming outcomes. For example, cells with shared CellTagD13 tags often had similar gene expression pattern changes...to use CellTag, you can find the CellTag libraries at Addgene. The Morris Lab's website also has several...

...blog has you covered. Happy voltage sensing!   Figure 3: Components of the types of voltage indicators...approaches. Functionally, the voltage sensitivity of the available VSDs has been successful even at the ... Several adaptations of this method have been to tag, cage, uncage, and trap the chemical fluor with the...single neuron level in vitro. In vivo monitoring has also been achieved with these sensors, but further...research tools for studying voltage within a cell are genetically encoded voltage indicators (GEVIs) and chemigenetic...Types of Voltage Indicators GEVIs can be divided up structurally into 2 main groups: voltage sensitive...occurring voltage indicator. To give an example, a genetically encoded phosphatase (voltage sensing) hydrolyzes...

...anti-FLAG tag antibodies By Ashley Waldron Of all the epitope tags we know and love, the FLAG tag (amino ...purify or image a recombinant protein, the FLAG tag has proven itself a good option for essentially all...Endothelial-specific AAV delivery Recombinant anti-FLAG tag antibodies Peptide-assisted genome editing for ...collection includes over 10,000 plasmids with a FLAG tag (!), but did you know that we distribute ready-to-use...FIQ or mStayGold (Ando et al., 2023). This variant has similar photostability and brightness compared to... of AAV9-X1.1 to that of AAV1. This viral vector has brain endothelial cell-specific tropism, with slightly...western blots, though the original parent clone (M2) has been widely used in a variety of applications. Find...

... a unique tag into a genomic site of choice via the mutational abilities of PolI3m. This tag would allow...used. EvolvR: key components The EvolvR system has two key components: the error prone PolI3M that’s...BioRender.com. The initial version of EvolvR has an editing window of ~17 nt from the nick site, which...directed mutagenesis to generate specific mutations in your plasmids Use REPLACER Mutagenesis to mutate...Mutagenesis is a tool that both evolution and molecular biologists use to tinker with DNA. Making changes...region. EvolvR, a CRISPR-Cas9 based targeted mutagenesis method developed by the Dueber Lab at Berkeley...potential applications. Challenges of existing mutagenesis methods Several continuous diversification methods...

... with containing chemical tags such as the SNAP-tag, Halo-tag, and CLIP-tag (Kohl et al., 2014) (Addgene... et al. "Ultrafast tissue staining with chemical tags." Proceedings of the National Academy of Sciences111.36... circuits with fluorescent proteins and chemical tags." eLife 7 (2018): e40350. PubMed PMID: 30454553.... an assistant professor at Kyushu University. He has a particular interest in the development of sensory...Imai is a professor at Kyushu University. His lab has been studying functional and anatomical aspects of...

...to their N-terminal His14-bdNEDD8 tag. After several washes, this tag can be cleaved by the bdNEDP1 protease...sacrifice and precludes genetic engineering of a tag or fusion with a reporter enzyme. For multicolor ...and decreases the distance between a fluorescent tag and the target antigen to about 2 nm, which can lead...antibody can be “labeled” by incubating it with a tagged nanobody. After this pre-incubation step, the primary...Label the Nanobodies: Purified nanobodies are then tagged with a fluorescent dye or HRP via maleimide-labeling...Control Protein Activity Learn more about protein tags Read about E. coli strains for protein expression... a primary antibody. B) Each arm of the antibody has an antigen binding site for a total of 2 binding ...

...disease. CRISPR has made genomic SDM relatively straightforward, but plasmid-based SDM has lagged behind...REPLACR-mutagenesis could save busy scientists a lot of time. Let us know how REPLACR-mutagenesis works ...Comparison of REPLACR-mutagenesis with other common methods for site directed mutagenesis   References 1. ...al. (2016). “REPLACR-mutagenesis: a one-step method for site-directed mutagenesis by recombineering.” ...Site-directed mutagenesis (SDM) is one of the key tools researchers use to prove causation in molecular...vitro assembly methods. A new method, REPLACR-mutagenesis, harnesses the power of bacterial recombineering... Plasmids After PCR). Current site directed mutagenesis methods Current SDM methods rely on in vitro ...

...contain a variety of N- and C-terminal tags (including His, S-tag, OneStrep, CBP, Trx, GST, Halo, MBP, ...promoter, N-terminal tag, coding sequence of the gene of interest, C-terminal tag, plant terminator, and...Ting’s lab has enabled progress on both fronts – as a genetic electron microscopy (EM) tag providing superior...of an isopeptide bond between 2 peptide tags, SpyTag and KTag. The group demonstrated the use of the SpyLigase...vector for bacterial mutagenesis or gene targeting Victor de Lorenzo's lab has engineered a modular...Building off their SpyTag/SpyCatcher system for protein-peptide locking, Mark Howarth’s lab has developed a ... superior subcellular localization of tagged proteins compared to traditional fluorescence microscopy,...

...combines components of SAM and SunTag activators, fusing the epitope tag of SunTag with the P65-HSF activation...SAM. This method has been shown to activate genes at 2- to 3-fold higher than SAM, SunTag, and VPR (Zhou..., Qi LS, Weissman JS, Vale RD (2014) A Protein-Tagging System for Signal Amplification in Gene Expression... for CRISPR activation, research in the area has continued to develop. These newer methods have not yet... of rearranging chromatin structure. This method has shown higher activation levels than SAM in some loci...popular activation methods (VPR and SunTag) (Chavez et al., 2016). SunTag Description Rather than using a ...activation levels are similar to that of SunTag. An advantage to this method compared to other notable...