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Showing: 31 - 40 of 99 results

  2. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...cloning: 1. Create Your PCR Product: Design standard primers (no need to add unique restriction sites on the...Pro tips Do not add 5’ phosphates to your PCR primers; you need that free hydroxyl group! You may want...

  3. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...unique barcoded Illumina-M13F bridging primer. These bridging primers are amplified and used to generate ... Type IIS restriction enzymes and standardized primers to clone your gene of interest into both sequencing...

  4. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ... great if you’re adding a restriction site to a primer, you only need to skim the alphabet on the side...helps you set up an optimal PCR depending on the primer sequence and the NEB polymerase used. Overall, ...

  5. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    Published
    March 30, 2017, 2:30 p.m.
    ... that much different from conventional duplexed primer probe assays often used in real time PCR. These...NHEJ. These assays include a forward and reverse primer to amplify the target locus, as well as a reference...

  6. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    Published
    Dec. 28, 2021, 2:15 p.m.
    ... the tenth day of CRISPR, Addgene gave to me: a primer on prime editing.  On the eleventh day of CRISPR...

  8. The Twelve Days of CRISPR

    Type
    Blog Post
    Published
    Dec. 22, 2020, 2:15 p.m.
    ... the first day of CRISPR, Addgene gave to me: a primer on prime editing.  On the second day of CRISPR,...

  10. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...suitable for the library in question. We will order primers that can amplify the insert of the library. This...PCR cleanup kit to remove any template DNA and primers.  After performing extensive QC on that sample,...
Showing: 31 - 40 of 99 results