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We narrowed to 749 results for: Tro;

Showing: 91 - 120 of 749 results

  1. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...Noninvasive Controlling Stimulation ExogenousEasily controlled EndogenousNot easily controlled Unique...spatiotemporal control of a small subset of neurons is necessary, use optogenetics. If broad control of whole...boundaries in neuroscience by granting targeted control over neuronal activity. While they serve similar...optogenetics differ are: Timing Targeted manipulation Controlling stimulation Invasiveness  Choosing the best ...Chemogenetics does not offer the same level of control over timing. Because chemogenetics relies on chemical... can reach target cells throughout the body. Controlling Stimulation Some studies rely on delivering reversible...stimulation to receptors. Optogenetics offers better control over these factors as the light source can be easily...

  2. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    Published
    July 21, 2020, 1:15 p.m.
    ... or chaotropic salts. Capillary electrophoresis Quantifying DNA using capillary electrophoresis is ... using gel electrophoresis. Except, it’s smaller. And automated. Unlike gel electrophoresis, you only ...wavelength that they maximally absorb. The spectrophotometer measures these absorbances using UV-transparent...absorb best at 280 nm and organic compounds and chaotropic salts maximally absorb at 230 nm. The A260/A280...specific for double strand DNA (as compared to spectrophotometric methods that measure all nucleic acids). ... sample concentration for you. Agarose gel electrophoresis Though not the quickest way to quantify DNA...methods can’t tell you and you don’t need a spectrophotometer or a fluorometer to quantify DNA this way....

  3. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    Published
    June 25, 2019, 12:54 p.m.
    ...have learned how to introduce genetic materials into cells in the lab. The introduction of foreign DNA or...research. There are multiple ways foreign DNA can be introduced into cells including transformation, transduction...plasmid of interest. Scientists can also use electroporation, the application of an electrical charge to...Transduction occurs when foreign DNA or RNA is introduced into bacterial or eukaryotic cells via a virus...Transduction is a common tool used by scientists to introduce different DNA sequences of interest into a bacterial...transduction. Scientists also use transduction to introduce foreign DNA into eukaryotic cells, like mammalian... process by which foreign DNA is deliberately introduced into a eukaryotic cell through non-viral methods...

  4. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 15, 2017, 1:24 p.m.
    ...Finsterbusch, Michaela, et al. "Patrolling monocytes promote intravascular neutrophil activation and glomerular...same laser) cooperate to cause a higher-energy electronic transition in a fluorescent molecule (see figure...epifluorescence microscope and GFP fusion proteins controlled by promoters specific to the immune lineage, ...movements in real time. This technique was first Introduced to immunology by three papers in the journal ...of pathogen-immune cell interaction relied on in vitro studies where it was difficult to apprehend the ... The video above depicts the infiltration of neutrophils expressing LysM-GFP (green) into a draining lymph...infection with S. aureus (red). You can then see neutrophils swarming around S. aureus and eating the bacteria...

  5. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    Published
    Jan. 17, 2018, 2:57 p.m.
    ...pouring in! While our lentiviral service is going strong, the AAV service has shown incredible growth this...EBFP-Cre Available in serotypes AAV1, AAV5, and AAV retrograde (the most popular form), this viral vector is...deliver DNA encoding Cre recombinase under the control of the synapsin promoter. It can be used to drive...genes to various brain regions. You can partially control which cells are successfully infected by an AAV...serotype, but occasionally you may want to further control where your gene of interest is expressed.  A cell-type...cell-type specific Cre can provide you with that control in a modular way. For example, say you would like...contains a Cre-dependent mCherry cassette under the control of an otherwise ubiquitous promoter. You could ...

  6. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    Published
    Aug. 6, 2019, 12:55 p.m.
    ... for introducing one modification into the mouse genome, they are not commonly used to introduce multiple...opposed to ordering control mice from a stock center. This serves as a better control since the experimental...series, we covered techniques that can be used to introduce genetic modifications into mouse embryos. But ...knockout lines and Cre-lox lines, and how to properly control for genetically engineered mice in your experiment...mutations. This is because as more mutations are introduced into a single embryo, the likelihood that a mouse...genetic engineering should be used to separately introduce each mutation independently into different mice...not a waste. Best practice is to use littermate controls in your experiment - use mice that were bred in...

  7. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...Promoters control the binding of RNA polymerase and transcription factors. Since the promoter region ...promoters can be regulated via positive or negative control. Positive Repressible: An activator protein is ...pJKR-H-tetR from the Church lab uses this system to control expression of GFP.)  To turn this inducible system...system, Gossen and Bujard created the tetracycline-controlled transactivator (tTA). They fused TetR with the...displays low promoter activity (~20% activity of the strong yeast promoter pTEF). As ethanol accumulates, it...interest to be expressed. These permit exquisite control of gene expression and tracing of gene expression... and Potter labs has refined the Q system and introduced the second generation QF2 activators. These activators...

  8. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    Published
    Aug. 3, 2023, 1:15 p.m.
    ...markers. Compensation/reference control(s) Compensation or “reference” controls are samples stained with a ...is not the same as your single color control. Reference controls: Beads or cells? Compensation beads are...mathematical algorithm and single stained reference controls to distinguish one fluorophore from another within...autofluorescence (you can see this in your unstained flow controls). Spectral flow cytometers include a tool called... or spectral flow. In spectral flow, reference controls tell the software to look for that exact signature...using conjugated dyes, it is critical that your controls and your sample antibodies are from the same batch... are a convenient tool to obtain a strong positive signal for a given fluorophore, as they contain a mixture...

  9. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type
    Blog Post
    Published
    Aug. 27, 2020, 1:15 p.m.
    ...right negative control is critical to the success of BiFC experiments. The ideal control is a mutated variant... work. You will also need to generate negative control constructs — more on this later. For more on constructing... measure the fluorescence of a single FP. Your control construct may generate a modest level of fluorescence...intensity of your BiFC constructs to your negative control to determine whether your PIPs actually do interact...has been compromised (Kodama and Hu, 2012). Such controls are necessary because split FPs can associate,...fragments themselves cannot be used as negative controls because their expression level, stability, and...structural information, constructing appropriate controls may requires that you empirically test a number...

  10. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    Published
    Nov. 17, 2016, 3:30 p.m.
    ...delivering control shRNAs, GFP, and rtTA from the Tet-on system. The first two can serve as controls in a variety...virus, and quality control data for each of the materials we distribute. Quality control info can be found...The viral preparations undergo rigorous quality control testing at Addgene meaning they come ready made...used to activate expression of a gene under the control of the tet operator. AAV AAVs are the workhorses...are already available allowing you to chemically control neuronal activity while studying your phenotype...of interest. Optogenetic tools for similarly controlling neuronal activity with light will be available...determined by the serotype. You can use these as controls to determine if your AAVs are getting to the right...

  11. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...generally it consists of a centromere-like region on the plasmid, a centromere-like region binding protein... the origin of replication and the replication control machinery (Need a refresher on the Origin of Replication... because they compete for the same replication control machinery within the cell. Most plasmids encode...two models for iteron-based plasmid replication control that are not necessarily mutually exclusive. In...plasmids, different negative regulation systems control plasmid replication of each plasmid using unique... complex than simply competing for replication control factors and tends to be more of a concern with ...2012).  During cell division, CBPs bind to the centromere-like region on each plasmid and pair the plasmids...

  12. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ...between recipients.   In vitro prediction of drug immunogenicity  The goal of in vitro prediction of immunogenicity..., 2020).  Evaluating a drug’s immunogenicity in-vitro helps researchers understand a drug’s safety and...depending on the person. However, in silico and in vitro assessment of immunogenicity can help evaluate risk...silico prediction algorithms fail to capture. In vitro evaluation of ‌therapeutic immunogenicity is typically...induction (Groell et.al, 2018). Thus, microscopic in-vitro models can be built that contain various components...components of the immune system. Figure 2: In-vitro models for immunogenicity prediction of therapeutic...efficacy.  Better together Combining in silico and in vitro approaches enables researchers to develop safer ...

  13. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    Published
    June 28, 2018, 3:01 p.m.
    ...positive control in our knock-in experiments as it works quite efficiently and serves as a good control for...pull-down assays include immunoprecipitation mass spectrometry (IP/MS) and chromatin immunoprecipitation (ChIP... protein complexes are then analysed by mass spectrometry to identify the interacting proteins. Similarly...the field, it has recently become possible to introduce DSBs and concomitant insertion of epitope tags...of plasmid vectors and/or rely on cumbersome in vitro synthesis of single guide RNAs (sgRNAs), are time-consuming...tracrRNA system is much more potent than the in vitro-transcribed single guide RNA (IVT sgRNA) approach...Direct comparison of Cas9 RNPs prepared with an in vitro-transcribed (IVT) sgRNA versus synthetic cr:tracrRNA...

  14. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ...spread of cancer cells. Besides these stromal cell modifications, stromal cells are also constantly communicating...communicator at the Portuguese science museum "Fábrica Centro Ciência Viva" in Aveiro, Portugal. If cancer was...inflammatory, angiogenic vascular, endothelial, and stromal cells - a rich soup of life encircling the tumor...caveolin 1 in breast cancer also induces changes of stromal cells in the microenvironment. This results in ...microenvironment is the immune cells. The contradiction is strong in this one. After all, the system built to protect...decrease the expression of TAK 1, an enzyme that controls apoptosis and regulates transcription. Without... cannot undergo apoptosis, thus multiplying uncontrollably and building up tumors. “Friendly” microenvironment...

  15. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    Published
    Nov. 6, 2018, 1:12 p.m.
    ...Transdifferentiation in vivo or in vitro in vitro Both in vitro and in vivo Epigenetic marks Complete...include retrovirus [2], lentivirus [4], adenovirus [5], Sendai virus [6], plasmid electroporation (episomal...redifferentiation into the cell type of choice in vitro is known as reprogramming [1]. The process can be...small molecules, and growth factors are used to control the cell fate of iPSCs and differentiate them into...the earliest examples of transdifferentiation in vitro where treatment of mouse fibroblasts with 5-azacytidine...original somatic cells [41]. The in vivo and in vitro methods are similar except that the vectors carrying...reprogramming process as they are propagated in vitro as part of the process. This opens up a wide range...

  16. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...use our negative control to compare exposure times of our samples. A prominent, strong signal has a shorter...negative control, and a weak signal has a longer exposure time closer to the negative control. Reproducibility...specificity and exposure time. We first have a negative control which does not have primary and only contains secondary...background noise. We then compare the negative control to a sample with the primary included to confirm...If the sample images similarly to the negative control, that informs us that the primary antibody is very...binding. If the sample differs from the negative control, such as showing a very bright signal only in the...that this antibody has specific binding and has a strong signal. We call the unique pattern of the antibody...

  17. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    ...appropriate for generating knockouts or knock-ins, or introducing mutations and tags, while a catalytically dead...conjunction with activator or repressor domains to control gene expression. Base editors can help you make...mismatch repair assay? Or is PCR followed by gel electrophoresis and/or next-generation sequencing more appropriate...system, you can then choose the best method for introducing those CRISPR components into your target cells...Plasmids containing gRNA and Cas9 are used in in vitro transcription reactions to generate mature Cas9 ...delivered to target cells using microinjection or electroporation. Transient expression of CRISPR components,...ribonucleoprotein complexes Purified Cas9 protein and in vitro transcribed gRNA are combined to form a Cas9-gRNA...

  18. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ...high false positives. Control vs. all samples Oftentimes there exists a control group and multiple experimental...significantly greater or lesser than the mean of a control group. If you only care if one group is different... want to measure if a sample is greater than a control), then a one-tailed test is suitable. Of course...subjects or populations (a treatment group vs. a control group), on the other hand, should be analyzed with...group being compared to a standard value (not a control group). This situation is best served by a one-...can test three or more groups (two samples and a control, for example) simultaneously, with up to two independent...these tests from ANOVA/MANOVAs. Quantifying and controlling for any covariance between manipulated independent...

  19. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...(4; brighter than control, 3; same as control (Cetn1/sgRNA1), 2; darker than control, 1; very dark). Brighter...your in vivo work is to screen you gRNA in vitro first. In vitro screening is a simple and fast method for...selecting the guide RNA, validating its efficacy in vitro, and using it in mouse embryos to generate gene ... many previously validated gRNAs at Addgene. In vitro screening for the best gRNA Figure 1: Validation...Validated gRNAs to Target Mouse Genes To begin the in vitro screening process, you should first develop primers...in vivo. Find Plasmids for Testing Your gRNAs in vitro at Addgene CRISPR/Cas9 gene editing in mice Now ...mammalian cells was being explored and it allowed control over each individual component of the system. However...

  20. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    Published
    Jan. 24, 2019, 2:24 p.m.
    ...can introduce the four Tetbow plasmids (Addgene #104102 - #104105) using in utero electroporation or with...#104108) (Figure 3) that can be introduced using in utero electroporation. Then at your preferred time points...multiple copies of the transgene cassette are introduced, stochasticity will result in a combinatorial...stochastic expression of XFPs is possible by simply introducing a mixture of XFP genes. Stochastic multicolor...labeling is possible when each of the XFP genes is introduced at ~2 copies per cell per color, following a ...Brainbow) (Sakaguchi et al., 2018) (Figure 1). Once introduced, fluorescent proteins were best imaged after ...procedure, find the protocol here.   Tips and troubleshooting your Tetbow experiment Plasmid concentrations...

  21. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ... fibers are drawn with microelectronics included in them for downstream control – this is important later...neural fibers developed by the Anikeeva lab are microelectronic fibers made from thermoplastic polymers that...optogenetic purposes, measure temperature, study electrophysiology, and deliver small molecules.   Fun fact:...(Orguc et al., 2020). Using a programmable microcontroller, NeuroStack coordinates optical stimulation...fibers as previously described, then measured electrophysiological potential afterwards. This measurement trended..., P., & Chandrakasan, A. P. (2020). Modular Optoelectronic System for Wireless, Programmable Neuromodulation... Anikeeva, P. (2019). Flexible fiber-based optoelectronics for neural interfaces. Chemical Society Reviews...

  22. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ...intersectional studies And more! Control AAV We’re always excited to add new control AAVs to our inventory since...popular control AAV encoding GFP. Check out our inventory of serotype testing AAV New control AAVs ...our entire AAV inventory. Our new AAVs include: Control AAVs for serotype testing jGCaMP7c for calcium ...scientists, we now offer trial size 20 µL samples of control AAV that scientists can use for serotype testing...Plasmid Serotype Name 119036  AAV1  pAAV-hsyn-flex-Voltron-ST DREADDs / Chemogenetics AAV We have also ...used in combination with Cre for intersectional control of specific neuronal populations. We now offer ... Addgene in the next 6 months, pending quality control. For an estimate on availability date or to be ...

  23. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    .... Find the Dolcetto lentiviral prep here Control AAV Control AAV allow researchers to not only interpret...many of our control plasmids in new serotypes, as well as a new Cre dependent GFP control plasmid for ...mediated neuronal control. The advantages of optogenetics lie in the spatiotemporal control offered by the...for their own experiments. To this end, we offer control AAV in smaller aliquots of 20ul for serotype testing...also offered as AAV1 (along with AAV PHP.eB, AAV Retrograde, AAV2, AAV5, AAV8, and AAV9). Once activated,... Addgene in the next 6 months, pending quality control. For an estimate on availability date or to be ... AAVrg pAAV-hDlx-GqDREADD-dTomato-Fishell-4 Controls Plasmid Serotype Name 114471 AAV2 pAAV-Ef1a-fDIO...

  24. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...type SOX2 with either a SOX2-VP16 (strong activator) or SOX2-HP1 (strong repressor) fusion protein, the authors...elavl3/HuC), microglial/macrophage (mpeg1.1), and astrocytic (gfap).This toolbox adds new neuronal tools to...to express the transcription factors from a polycistronic cassette. However, it was unknown whether variations...variations in gene expression from these polycistronic cassettes could lead to comparable experimental...Woltjen Lab compared different variants of the polycistronic cassettes and discovered reprogramming discrepancies...variation in the Klf4 isoform (KLF4s or KLF4L). Polycistronic cassettes that contained KLF4s had overall diminished...reprogramming and global gene expression patterns. Polycistronic cassettes that contained the KLF4L isoform had...

  25. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ... and destroyed by CRISPR/Cas9 during transfection. Making the double strand break and introducing the ...individual proteins under endogenous regulatory control. But even with the advent of CRISPR/Cas9 technology... in human cell lines. Furthermore, the quality control necessary to ensure the edited cells are behaving...the tag through homology directed repair To introduce the fluorescent tag to the cells, we used CRISPR/Cas9...with the cut, we trick the cell into using the introduced donor plasmid as a repair template. This results...and the donor plasmid into the cells we use electroporation, a technique that briefly destabilizes the ...Figure 3: Inducing the double strand break and introducing the tag. Created with BioRender.com. Plasmids...

  26. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    Published
    Dec. 15, 2016, 3:57 p.m.
    ...system to modify your target DNA, you first electroporate a linear donor DNA substrate (either dsDNA or...and SbcCD nucleases from digesting linear DNA introduced into the E. coli. Exo: Exo is a 5’→3’ dsDNA-dependent...Expression of lambda red recombination genes. Electroporation of substrate DNA and outgrowth of bacteria....dsDNA recombineering substrates to avoid errors introduced during PCR. ssDNA Substrate ssDNA substrates ...that avoid MMR activation.  The first way is to introduce a C/C mismatch at or within 6 base pairs of the...1% or 25-50% when activation of MMR is avoided Introduce C/C mismatches or silent changes in wobble codons...these will be discussed in more detail below).  Controlling expression of Red proteins is critical for minimizing...

  27. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...would like to introduce the edit. Finally, you must stipulate what sequence to introduce, which is done...guide’s recognition sequence is destroyed via mutation. By introducing a mutation in either the PAM or...Identify where in the genome you would like to introduce your knock-in. Then choose which Cas enzyme to...region of a gene, make sure that the PAM edit you introduce is a silent mutation, so you don’t accidentally...to your culture media prior to and after Cas9 introduction increases HDR, just make sure to remove the ...homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery. eLIFE. (2014)...efficient homology-directed repair and termperature-controlled genome editing. Nat. Com., 8(2024), (2017). 10.1038...

  28. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...emission Before we design your first panel, we will introduce some background on the color spectrum and the ... photons are absorbed by matter, they promote electrons within atoms to a higher energy state. This process... called excitation. After a short period, the electrons revert to a lower energy state, whereby a photon...those of absorbed photons. When photons excite electrons of a fluorophore, they can do so at a range of...by the cytometer. Brighter fluorophores have a stronger signal. Table 1: Degrees of brightness for a few...absorbed and then emitted, which creates a relatively stronger — or brighter — signal.   Figure 2: Excitation... real-life advantage of a brighter fluorophore? Strong brightness makes it easier to distinguish a signal...

  29. New Viral Vectors - Fall 2024

    Type
    Blog Post
    Published
    Oct. 3, 2024, 1:15 p.m.
    ...-GFP AAV9-X1.1 Controls Edward Boyden New serotype pAAV-hSyn-EGFP AAV PHP.eB Controls Bryan Roth New...CN3905) AAV PHP.eB Controls Jonathan Ting New viral prep pAAV-CaMKIIa-EGFP AAV1 Controls Bryan Roth New ...minBG-SYFP2-WPRE3-BGHpA (Alias: CN3044) AAV PHP.eB Controls Jonathan Ting New viral prep AiP13038 - pAAV-AiE0743m...minBG-SYFP2-WPRE3-BGHpA (Alias: CN3038) AAV PHP.eB Controls Jonathan Ting New viral prep AiP12408 - pAAV-AiE0600m-minBG-SYFP2...pAAV-AiE0600m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2408) AAV PHP.eB Controls Jonathan Ting New viral prep AiP13863 - pAAV-AiE0888m_C4...minBG-SYFP2-WPRE3-BGHpA (Alias: CN3863) AAV PHP.eB Controls Jonathan Ting New viral prep AiP13905 - pAAV-AiE0441h...New serotype pAAV-Ef1a-DIO mScarlet AAV5 Controls Karl Deisseroth New serotype pAAV_hSyn-PdCO-mScarlet-WPRE...

  30. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    Published
    Sept. 1, 2020, 1:15 p.m.
    ...deliver the mini-dystrophin gene to a mouse model of muscular dystrophy. While mini-dystrophin expression levels... to deliver a 6 kb mini-dystrophin gene to a mouse model of muscular dystrophy. Three different splice... and expression of the full-length transgene in vitro and in vivo (Allocca et al., 2008). Shortly after...levels were lower than expression of wild-type dystrophin in normal muscles, mice treated with the overlapping...achieved ~80% of muscle cells expressing mini-dystrophin.  3. Hybrid  Overview: The hybrid approach combines... can be from one of the transgene’s endogenous introns or comprised of non-endogenous sequences, such ...Gene Therapy of mdx Mice With Large Truncated Dystrophins Generated by Recombination Using rAAV6. Molecular...
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