Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 149 results for: pcr

Showing: 91 - 120 of 149 results

  1. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ... have DNA left over for other applications, like PCR, cloning, transfection, or long-term storage. But...

  7. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...Ehrhardt, A., & Baiker, A. (2012). Universal real-time PCR for the detection and quantification of adeno-associated...aliquots of virus. AAV titration by qPCR Overview: AAV titering by qPCR quantifies the number of genome-containing... hours for the qPCR run and data analysis. Refer to this Addgene AAV Titration by qPCR Protocol for more...remember to make one or two small aliquots to use for qPCR titering. This helps avoid multiple freeze/thaws... included. While the physical titer generated by qPCR is useful, I test a few different concentrations...

  13. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...genes replaced.  Additionally, 2A peptides could be PCR-cloned between your genes of interest and you can...

  16. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...targeting region from the vector. Alternatively, PCR-generated or commercially synthesized linear dsDNA...

  19. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...restriction enzyme (RE) recognition site, RE analysis of PCR product could be used to detect success. Although...

  21. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...generate Illumina sequencing libraries using a 2-step PCR-based protocol and are compatible with single-end...biology 11.1 (2015): 31-37. pCri system The Gomis-Ruth lab designed the pCri system to simplify the protein...the repository: FX Cloning Kit BIOFAB Plasmid Set pCri System Cell Free Protein Expression Kit FREQ Seq...depending upon your specific protein folding needs. The pCri system has recently been used to study a matrix ....8 (2015): 4728-4740. You can find more info on pCri in Mary Gearing’s blog post on the system Easing...Easing the Protein Purification Process with pCri Cell-free protein expression kit Cell-free expression ...

  22. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...Addgene Blog Verify Your Constructs with Colony PCR Read about Tags for Protein Purification Resources... use by the Rinehart lab: pCRT7 NT TOPO E17TAG GFP and pGEX-6P-1. The pCRT7 NT TOPO vector contains a ...68292 C321.ΔA bacterial strain – Addgene #68306 pCRT7 NT TOPO E17TAG GFP plasmid - Addgene #68295 SupD...

  24. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ..., SacI restriction site and a linker region. The PCR product is then ligated into the MCS/SacI site in...can occur in this system if a small contaminating PCR product formed from primer dimers and containing ...clone. ORFs from an ORFeome Gateway library or any PCR-amplified ORF cloned into pDONR221 can be expressed...

  27. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...and the sequence of gene of interest in separate PCR amplication reactions for recombination in SLIC cloning...the ccdB cassette, which is not copied during the PCR amplification step, so that only vectors with the...or multiply phosphorylated proteins (B40 OTS and pCRT7-GFP). To demonstrate the improvements of the system...

  29. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...such as physically carrying out experiments like PCR and running gels, but in research you also experience...

  30. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...vectors using BsaI, and promoter-gRNA fragments are PCR amplified for cloning into one of three Zeamays codon-optimized...
Showing: 91 - 120 of 149 results