We narrowed to 155 results for: pcr

...reaction. This will allow you to quantify the amount of PCR product that comes from amplification of the original... titer are direct p24 measurements via ELISA and qPCR for viral RNA. Measuring p24 levels in a viral prep...first converted to cDNA and then quantified using qPCR primers targeting specific viral components such...assays depending on the vector backbone. When using qPCR based methods for viral titration consider using...most accurate method of lentiviral titration uses qPCR to measure the number of proviral copies that have...cell line known to contain 1 integrated copy of the qPCR target gene. Developing the proper control line ...

...include places where DNA is being created, such as PCR amplification or bacterial growth. Created with BioRender.com...

...screen for this specific recombination outcome using PCR and then generate a monoclonal cell line with the...

... have DNA left over for other applications, like PCR, cloning, transfection, or long-term storage. But...

...insertions/deletions using a mismatch repair assay? Or is PCR followed by gel electrophoresis and/or next-generation...

...the purified virus should be ~1.3 Quantitative PCR: the number of viral DNA packaged in virions is determined...

...efficacy, we have used both Western blotting and PCR, depending on the level of endogenous expression....

...it’s so easy once you are in the lab. There is no PCR, no gel purification; the whole cloning process is...

...purifying plasmid DNA from bacteria or cleaning up a PCR product, a common material you are trying to remove...

...titrated following purification using qPCR or digital droplet PCR. Insect cells Insect cells are a less...

...plate overnight Screen colonies phenotypically or by PCR/sequencing While CRISPR-transposons are powerful...

...bound genomic locus, which they then confirmed by PCR. Subsequent mass spectroscopy studies allowed for...

...Ehrhardt, A., & Baiker, A. (2012). Universal real-time PCR for the detection and quantification of adeno-associated...aliquots of virus. AAV titration by qPCR Overview: AAV titering by qPCR quantifies the number of genome-containing... hours for the qPCR run and data analysis. Refer to this Addgene AAV Titration by qPCR Protocol for more...remember to make one or two small aliquots to use for qPCR titering. This helps avoid multiple freeze/thaws... included. While the physical titer generated by qPCR is useful, I test a few different concentrations...

... selection. Screening of transformants by colony PCR confirmed the insertion of the Lambda insert at the...

...both edited and unedited (control) populations is PCR-amplified and Sanger sequenced. ICE compares these...

...detected directly by Northern blot analysis or RT-PCR. Uses of the minigenome system Minigenome systems...

...genes replaced.  Additionally, 2A peptides could be PCR-cloned between your genes of interest and you can...

...(specific to your target) to screen colonies via PCR. A 354-bp product confirms successful insertion....

... What is a Plasmid? How to Design Primers for PCR Agarose Gel Electrophoresis Inoculating Liquid Bacterial...

...targeting region from the vector. Alternatively, PCR-generated or commercially synthesized linear dsDNA...

... individual gene edited clones are identified by PCR. Then, if necessary for subsequent experiments, the...

...Overall, it worked. We pulled it off! Check out the PCR Primer video! Learn: Producing videos like this would...

...analyzers, and any ultra sensitive equipment (RT-PCR, Microscopy, Fluorescence Scopes, Flowcytometers,...

...vector particle components or performing quantitative PCR titration of vector DNA in samples harvested 24 hours...

...restriction enzyme (RE) recognition site, RE analysis of PCR product could be used to detect success. Although...

...generate Illumina sequencing libraries using a 2-step PCR-based protocol and are compatible with single-end...biology 11.1 (2015): 31-37. pCri system The Gomis-Ruth lab designed the pCri system to simplify the protein...the repository: FX Cloning Kit BIOFAB Plasmid Set pCri System Cell Free Protein Expression Kit FREQ Seq...depending upon your specific protein folding needs. The pCri system has recently been used to study a matrix ....8 (2015): 4728-4740. You can find more info on pCri in Mary Gearing’s blog post on the system Easing...Easing the Protein Purification Process with pCri Cell-free protein expression kit Cell-free expression ...

...Addgene Blog Verify Your Constructs with Colony PCR Read about Tags for Protein Purification Resources... use by the Rinehart lab: pCRT7 NT TOPO E17TAG GFP and pGEX-6P-1. The pCRT7 NT TOPO vector contains a ...68292 C321.ΔA bacterial strain – Addgene #68306 pCRT7 NT TOPO E17TAG GFP plasmid - Addgene #68295 SupD...

...expressed them along with Cas9 in the T-cell line 2D10. PCR amplification and Sanger sequencing of pooled samples...

..., SacI restriction site and a linker region. The PCR product is then ligated into the MCS/SacI site in...can occur in this system if a small contaminating PCR product formed from primer dimers and containing ...clone. ORFs from an ORFeome Gateway library or any PCR-amplified ORF cloned into pDONR221 can be expressed...

... repair templates using Addgene plasmids. Design PCR primers to add T7 promoter-left homology arm and ...