...process samples at the picnic table before dark, a bison wandered into our campsite. We had to abandon our...our samples and move farther away until the bison meandered off. In the end we were able to safely get...get back to our lab work. Figure 3: When a bison wanders into the campsite, leave your samples behind...ThermoCas9 from the van der Oost lab. Phylogenetic comparison indicates that like GeoCas9 and ThermoCas9, IgnaviCas9...

...end; cause, effect; lesson, learning. Storytelling is a crucial part of interpersonal communication, which... it came time to add author details a different person in the lab wanted to be the first author. When ...protagonist will be you. Make sure to highlight what lessons you’ve learned and what knowledge you’ve gained...

...   In fact, there are a lot of good reasons to stay and good reasons to go.  Here are some questions and...especially race and gender) Interpersonal issues can create interpersonal issues (one bad apple can change...

...Although CRISPR/Cas9 is all the rage, they had another reason to test this system. It’s difficult to determine...require multiple transduction events. For these reasons, iTOP is best suited to binary systems in which... size associated with viral vectors. For these reasons, iTOP, especially in tandem with genome editing... VP22 nuclear homing an artifact? Lundberg M, Johansson M. Nat Biotechnol. 2001 Aug;19(8):713-4. PubMed...

... T. M., Edwards, A. M., Healy, L. M., Robertson, J., & McPherson, P. S. (2019). Implementation of an antibody...perform as well in other conditions. For these reasons, it is essential to test the selectivity of your...cannot prove antibody selectivity, and for that reason, it is best practice to combine correlation with...C., Bandrowski, A., Virk, H., Edwards, A. M., McPherson, P., & Laflamme, C. (2023). Scaling of an antibody...

...Thanks to our guest blogger Cameron MacPherson! Cameron R. MacPherson is Lead Data Scientist for the Milieu...post was contributed by guest blogger Cameron MacPherson at the Institut Pasteur CRISPR software and the... by their focus on specific organisms. For this reason I generally recommend that laboratories that have...CRISPR technology to a new organism: For obvious reasons you can rule out any tool that limits you to a ...

...fluorescent proteins everyday.  The late Michael Davidson and Roger Tsien played enormous roles in enabling...their legacy, we recently launched the Michael Davidson and Roger Tsien Commemorative Travel Awards. These... we’re proud to announce our first two Michael Davidson and Roger Tsien Commemorative Travel Awardees:...Plasmids from the Tsien Lab Browse the Michael Davidson Collection ...

...contributed by guest bloggers Samantha Zyontz and Neil Thompson from the MIT Sloan School of Management. Professor...depositing a plasmid with Addgene, in contrast to other reasons for the correlation. However, determining this ...to our guest bloggers, Samantha Zyontz and Neil Thompson. Special thanks to Addgenies Pamela McA'Nulty ...page: https://ssrn.com/author=1232466.     Neil Thompson is an Assistant Professor of Innovation and Strategy...

...When our Director of Biology, Lianna Swanson, suggested that I might be able to attend the 10 Years of...degeneration; Laurenz Studer discussed his work with Parkinson's Disease; Hideyuki Okano presented ground-breaking...clinical trials for ALS.   I have an uncle with Parkinson's, a half-brother who is a quadriplegic due to ... are key to the research, but they are also the reason this research is happening. Stem cells scientists...

...make for an excellent model organism for a few reasons: Affordable to culture and easy to work with ...established in yeast, including a widely used Parkinson’s experimental model. In fact, approximately 25%...of the uses outlined below. Biology They say comparison is the thief of joy, but I think it’s acceptable...aren’t going anywhere anytime soon, and for good reason! However, there are other players in the game (...

... by Kendall Morgan and updated in 2022 by Lucie Wilson. Lucie is an Addgene co-op from Northeastern University...in monoallelic diseases In 2013, a team led by Jinsong Li from the Chinese Academy of Sciences found that...fibrosis (8).   The difference with CRISPR/Cas9 in comparison to other genome editing techniques is its ease...s Disease.” Journal of Alzheimer's disease & Parkinsonism. https://doi.org/10.4172/2161-0460.1000439 6...

...Levskaya A, Chevalier AA, Tabor JJ, Simpson ZB, Lavery LA, Levy M, Davidson EA, Scouras A, Ellington AD, Marcotte...Chemical effectors are suboptimal for a number of reasons, including their potential toxicity and cross-reactivity...genetic edge detection program. Tabor JJ, Salis HM, Simpson ZB, Chevalier AA, Levskaya A, Marcotte EM, Voigt...

...come into being through the actions of a single person but through the coordinated efforts of many individuals...collection page, we certainly don't expect that one person will write all the copy, design every icon, curate...page. That would be entirely too much work for one person, it would take forever, and the end product would...who can do the job. It is good to delegate so the person doing the task will have to learn and stretch a...

...in the form of a protocol, and you can add in personalized steps like “check drawers for light-sensitive....      References and Resources Images used Roberson Lab, UPenn. Western blotting. Retrieved Feb. 7... 7, 2022. https://www.med.upenn.edu/robertsonlab/assets/user-content/documents/Western%20Blotting.pdf  ... Rachel Leeson...

...PMID: 29553576. 2. Kittleson, Joshua T., Sherine Cheung, and JChristopher Anderson. "Rapid optimization..., and ColE2 plasmids) as cell division slows (Kittleson et al., 2011). Segall-Shapiro et al. showed that...References 1. Segall-Shapiro, Thomas H., Eduardo D. Sontag, and Christopher A. Voigt. "Engineered promoters...

...AAV pRDA_889 (ITRs from AAV2) Mammalian Transposon pRDA_757 (Piggybac; constitutive expression... drosophila) Delivery type (lentivirus, AAV, transposon, Plasmid) Destination vector (dropdown options..., Bosch, J. A., Zirin, J. D., Viswanatha, R., Sontheimer, E. J., Goodale, A., Greene, M. A., Green, T.... Rachel Leeson...

...also probably thought about why certain posters resonate with you (or not). It is important to reflect ...length, and will be more difficult to read. The Thompson Rivers University Library provides useful tips...scientific poster design was conceived by Mike Morrison, a psychology doctoral student at Michigan State... avoiding red and green is a good idea for this reason but there are many other ways to make your poster...

...    What is this FRET you speak of? Förster Resonance Energy Transfer (FRET) was originally described...has led to the alternative acronym Fluorescence Resonance Energy Transfer. Unlike the typical excitation...Müller, Sara M., et al. "Quantification of Förster resonance energy transfer by monitoring sensitized emission... Jason Niehaus...

...national levels. These skills will also help the person mentor others more successfully. But how does one...phone calls. This has its challenges as meeting in person is often much more productive and makes it easier... previous formats but I learnt to overcome the personal frustration and submit reports in the format requested...voice may not always be perfectly aligned with my personal viewpoint but, as a leader in the NPA, I had to...

...edit, it simply becomes impossible (or at least unreasonable) to test them all. This is where PRIDICT comes...find their way into the hands of users. For this reason, we made our tools available via the PRIDICT website...A. A., Koblan, L. W., Levy, J. M., Chen, P. J., Wilson, C., Newby, G. A., Raguram, A., & Liu, D. R. (2019.... M., Pallaseni, A., Kuzmin, I., Raudvere, U., Peterson, H., Liberante, F. G., & Parts, L. (2023). Prediction...

...proteins on the cell surface. Figure 1: Comparison of common antibody, HCab, and nanobody. For more...starting library size (up to 1012 members) in comparison to other display systems using phage and yeast...Egloff P, Hutter CAJ, Kuhn BT, Bräuer P, Newstead S, Dawson RJP, Geertsma ER, Seeger MA (2020) Generation of... Gera J, Gmür S, Spies P, Gygax D, Geertsma ER, Dawson RJ, Seeger MA (2018) Synthetic single domain antibodies...

...PMID: 31891733. Brechmann NA, Schwarz H, Eriksson PO, Eriksson K, Shokri A, Chotteau V. Antibody capture....106. PMID: 33151027; PMCID: PMC7665837. L'Abbé D, Bisson L, Gervais C, Grazzini E, Durocher Y. Transient...Chen E, Haidar JN, Srivastava A, Goldstein J. Comparison of predicted extinction coefficients of monoclonal...

...the most successful scientists have outgoing personalities to supplement their intellectual prowess and... era of social media and self-promotion, and personality is playing an increasingly important role in ... that produced by the confident and flamboyant Watsons.” In Part Four, “New Science,” Zimmer tackles more...little bit about many different things (part of the reason Addgene has been such a great fit for me!), the...

...identify it as a personal strength until it is identified by another person. Similar reasoning may also apply...convey your empathy and your awesome solution to the person over email. Examine your current and past job ...

... yet retroviruses are RNA-containing viruses  (Robinson et al., 1965)! You may be beginning to realize...Brian O'Neill, with thanks to Addgenie Rebecca Hutcheson for her assistance with lentiviral replication...PMID: 26225354. PubMed Central PMCID: PMC4515362 Robinson, W. S., Pitkanen, A., & Rubin, H. (1965). The ... PMCID: PMC355630 Faras, A. J., Taylor, J. M., Levinson, W. E., Goodman, H. M., & Bishop, J. M. (1973)...

...maximise the time you are enjoying both work and personal life. There are no easy answers to negotiating...my colleagues and me. To that end, here are my personal 10 commandments for making it all work – most ...the “jobs” that take you away from having your personal time. Someone else can clean your house, pick ...you can’t make it to dinner this week. One swim lesson missed can mean a world of mental relaxation. And...

...restraining further replication.   Figure 3: Comparison of replication regulation mechanisms of stringently...regulation! This post was originally written by Jason Niehaus in December 2015 and was updated by Emily...464.1998 Grimwade, J. E., Rozgaja, T. A., Gupta, R., Dyson, K., Rao, P., & Leonard, A. C. (2018). Origin recognition.../doi.org/10.1093/nar/gky457 Park, K., Han, E., Paulsson, J., & Chattoraj, D. K. (2001). Origin pairing...

...fluorescent biosensors. Aptabeacons Fluorescence resonance energy transfer (FRET) technology has been studied... sensitive detection assay. In 1999, Grate and Wilson paired laser cleavage and an RNA aptamer that bound... May 15;26(9):3725-36. PubMed PMID: 21419619. 9. Song, Kyung-Mi, et al. “Aptamers and Their Biological... 22368488. PubMed Central PMCID: PMC3279232. 10. Song, Shiping, et al. “Aptamer-based biosensors.” TrAC...

...biologist some time… months, in fact. In the Lee-Parsons lab, we are investigating how the production of...protein of interest. Find all plasmids from the Lee-Parsons lab. Test different leaf infiltration methods ...(Germany), before starting his work in the Lee-Parsons Lab.     References D'Aoust, Marc-André, et al.... De Ruijter, N. C. A., et al. "Evaluation and comparison of the GUS, LUC and GFP reporter system for gene...

...relevant to you. Elect a devil’s advocate. This is the person whose job it is to disagree with the group. They...sure we have all experienced a meeting where one person speaks up after a group consensus and is immediately...entire company. It is important to note that the person who submitted the idea did not have to be in charge...always done it this other way,” you probably have reason to continue advocating for your idea. Our product...

...host tropism of your virus, there are many other reasons when you might pseudotype your virus:  Lowering...a cell surface to endocytose into the cell. The reason VSV-G is so commonly used is that it is good at...Sandoval, 2017). This high titer is one of the reasons VSV-G is used to transduce stem cells and neurons...https://doi.org/10.1093/hmg/10.19.2109 Sandrin V, Boson B, Salmon P, Gay W, Nègre D, Le Grand R, Trono ...

...10.1038/nature09523  Ibraheim R, Song C-Q, Mir A, Amrani N, Xue W, Sontheimer EJ (2018) All-in-one adeno-associated...can be turned off by anti-CRISPR proteins. The Sontheimer lab used NmeCas9 with AAV to edit two different...0  Kim E, Koo T, Park SW, Kim D, Kim K, Cho H-Y, Song DW, Lee KJ, Jung MH, Kim S, Kim JH, Kim JH, Kim ...

...believe GMOs are safe for human consumption in comparison to 37% of the general public (5, 6). Similarly... the public (8–13). Facing the possibility that reason is losing ground in public discourse, many scientists...politicians to interpret it for them. Other popular reasons to march include: General inclusion of science...policy should have more weight than how any one person feels about it. And yet research shows that thoughts...

...fellow at the Morgridge Institute for Research in Madison, Wisconsin. It is commonly cited that approximately...enzyme function, protein interaction, etc. by comparison to non-phosphorylated or ‘wild type’ control ...dephosphorylation reaction can be tracked through the comparison of phospho:non-phospho species on PhosTag gels...fellow at the Morgridge Institute for Research in Madison, Wisconsin. She is particularly interested in the...

... make the best case for your candidacy. A major reason that a lot of candidates either get no interviews...understand your cover letter and research statement. My personal litmus test was whether my wife could explain ... please introduce yourself to me by email or in person. If you feel compelled to repay me in some way,...job searches. If you feel the urge to repay me personally, I will gratefully accept a very hoppy pint of...

...nuclear- or DNA-binding proteins, you may need to sonicate your lysates in order to release the proteins ...you’re using chemical blockers, you can run a comparison of different formulations instead of concentrations...temperature, and/or buffer to increase lysis Add sonication step to protein lysis Splice variants Check...lysing buffer/adjust lysing buffer strength Sonication based lysing protocol Subcellular fractional...  Rachel Leeson...

...Addgene. Klann TS, Black JB, Chellappan M, Safi A, Song L, Hilton IB, Crawford GE, Reddy TE, Gersbach CA...publication at Addgene. Maeder ML, Angstman JF, Richardson ME, Linder SJ, Cascio VM, Tsai SQ, Ho QH, Sander...Sander JD, Reyon D, Bernstein BE, Costello JF, Wilkinson MF, Joung JK (2013) Targeted DNA demethylation... this publication at Addgene. Mendenhall EM, Williamson KE, Reyon D, Zou JY, Ram O, Joung JK, Bernstein...

... appear within a range of wavelengths. For that reason, we speak of excitation and emission spectra. Both... happens due to a process called Fluorescence Resonance Energy Transfer (FRET).   Figure 1: Emission...fused to CD45, but is separately expressed. (The reason for having a separately expressed transduction ...difficult. I chose BV421 for the following two reasons: 1) The emission spectrum of BV421 is far enough...

... work, funding for science, and numerous other reasons. It is important to remember that we, scientists...Albany, NY, USA 42.6526°N 73.7562°W – Nicholas Thompson Over the last few years, there's been a disturbing...positive event that I hope happens again. Nicholas Thompson is a PhD Candidate studying Nuclear Science and...enforcement, and, although there was at least one person vocally protesting us, he was not threatening in...

...Many thanks to our guest blogger, Doug Richardson! Doug Richardson is the Director of the Harvard Center...This post was contributed by Doug Richardson, Director of the Harvard Center for Biological Imaging and... 2, 932-940 (2005). PubMed PMID: 16299478. 9. Richardson DS, Lichtman JW. Clarifying Tissue Clearing. ...