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We narrowed to 602 results for: ACE

Showing: 151 - 200 of 602 results

  1. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    Published
    Dec. 20, 2017, 2:33 p.m.
    ...remember that industry career paths are not the only place to develop “industry related skills”. Many career...self-driving cars, tissue engineering for organ replacement).  In addition to presenting your expert skills...Google Scholar account Sanitize your LinkedIn, Facebook profile, online presence (if you need to).  Immigration...for international candidates. Being able to “put a face to a name” will help a candidate get looked at and...investment banking and venture capital areas.  We place individuals into roles in areas including: research...

  2. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...originally published in Nature, is a “search-and-replace” genome editing technique that mediates targeted... efficient due to its lower dependence on PAM placement. Fewer byproducts and more efficient than homology-directed... formation slightly. Designing the sgRNA with a spacer that only binds the edited strand, as in the PE3b... & Liu, D. R. (2022). Programmable deletion, replacement, integration and inversion of large DNA sequences..., Raguram, A., & Liu, D. R. (2019). Search-and-replace genome editing without double-strand breaks or ...

  3. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    Published
    June 30, 2020, 1:15 p.m.
    ...both conferences, there was a Q&A section in the interface where you can type in questions for the speaker...posters could be viewed publicly and stored in one place if the participants used the conference hashtag ... says.Depending on the meeting, there may be a “place” for networking within the app. For example, the... an on-site conference, you really have to make space for it,” says Bachle. “On the flip side, you can...

  4. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ...and novelty in scientific research and in any workspace. Re-design lab meetings with a focus on discussion...making lab meetings more interactive and creating space for discussion at the end of each talk - time for...remotely in science are endless. Let’s adapt and embrace new ways of working, reconnect as human beings ... purposeful and creative collaboration in the workplace. There are many other ways to encourage collaborations...

  5. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...Biology and, on occasion, would meet in the same space where BosLab exists today. Back then, it consisted...Kendall square, but we saw the potential in the space and decided to reactivate it. We started out by ...projects at BosLab. Some are entrepreneurs using our space to perform early-stage experiments. For example,... visit our website and follow us on twitter or facebook. If you are local, join us for our next event ...

  6. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...example the Phage Assisted Continuous Evolution (PACE) method requires constant turbidity of the culture...its native endonuclease activity degrades the displaced strand.   Figure 1: EvolvR consists of ... of PolI3m. This tag would allow researchers to trace the progeny of a single starting cell. Additionally...generate specific mutations in your plasmids Use REPLACER Mutagenesis to mutate your plasmid Resources ...

  7. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ... by spaces. File names with more than one word should be named in the camelCaseFormat since spaces between...analyze large data sets (myself included) and we are faced with finding the best system to document these types...the basics. However, these notes take up a lot of space and don’t tell you why you did something or what...

  8. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    Published
    Oct. 1, 2019, 1:21 p.m.
    ...Personalize all connection requests LinkedIn is a place to build relationships. If you don’t add a personal... talked about (or how good the beer was at that place, for example). This is also a great way to keep ...profile and not updating it. LinkedIn may actually replace the resume some day (I’ve already abandoned my ...have to be a headshot in a suit, but this is not Facebook so no pets or kids. Try looking a bit over your...

  9. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...amazing affinity reagents we just didn't have the space to give proper credit to. Thankfully, as Addgene's..., or be able to access an epitope folded into a space that would size-exclude an antibody. In imaging ...eukaryotic cells, allowing for them to be used as intracellular inhibitors.  Aptamers are not proteins at all.../neu and CD16 Exhibit Improved Tumor Lysis When Placed in a Divalent Tumor Antigen Binding Format. Journal...

  10. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    .... Everything will go wrong Whenever an order is placed on the Addgene website, it sets off a chain reaction... need to be repeated. We also now have a good interface to begin tracking analytics data for lab procedures...at some point, decide early on what to do in a namespace collision. It may even be worth it to have extra... you're solving, but consistency is key. Some namespace collisions you may encounter: vector, sequence...

  11. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...repeats separated by an 8-bp long asymmetric core spacer sequence. The asymmetry in the core sequence gives... double-strand DNA break occurs within the core spacer of each loxP site and the two strands are ligated...loxP sites is reversed.   Deletion: If the sites face in the same direction, the sequence between the ...been created, which contain a unique asymmetric spacer "NNNTANNN", where "N" indicates which bases may...

  12. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...edit. Figure 2: Cytidine deamination takes place on the free strand of DNA and converts a C to U ..., ABE8s resulted in 1.5-fold more editing at protospacer positions A5-A7 and 3.2-fold more editing at ...Joung’s lab created a dual-deaminase editor called SPACE (synchronous programmable adenine and cytosine editor...mechanism than base editing, has allowed scientists to replace entire sequences of varying lengths. Base editing...and adenine base editors Grünewald et al., 2020 SPACE   Fused cytosine and adenine base editors Walton...

  13. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ...PubMed search option in early 2020 and eventually replace the existing version of PubMed.  Key features This...references sections of a PubMed entry are great places to look for more articles on your topic of interest...Twitter to promote their work, so it’s also a good place to look for publications. Bonus: papers have to ...automatically update as papers are published, similar to Facebook or Instagram. Key features Setting up a personalized...

  14. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ..., if these variants are fused to the MLV Gag in place of SpCas9. gRNA A gRNA targeting a DNA sequence ... charge repulsion between virions and the cell surface, and also helps form DNA and retroviral-particle...components. However, this sometimes requires intracellular injections into the cytoplasm or pronucleus ...avoid this pitfall since they are injected into the space surrounding the zygote, where they can then fuse...

  15. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...structures, etc., as well as the presence of a Protospacer Adjacent Motif (PAM) sequence. If the gRNA sequence...extent NYN. This increases the flexibility of gRNA placement significantly over SpCas9 and more so than Cas9s...Engineered CRISPR-Cas9 nuclease with expanded targeting space. Science 361,1259-1262(2018). DOI:10.1126/science.aas9129...

  16. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...ultrafugation Overview: Iodixanol gradient ultracentrifugation uses a gradient of different concentrations...during AAV production is overlaid. Following ultracentrifugation, the AAV-containing 40% fraction is collected...day. Refer to the AAV Iodixanol Gradient Ultracentrifugation Protocol for more details. Pro tips Watch...transfer plasmids. It’s easy to contaminate a workspace with plasmid, not that I would know about this...

  17. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...contained all 64 possible NNN PAM sequences adjacent to a protospacer. If ω-dCas9 could recognize a library ...other CRISPR enzymes require a short PAM sequence adjacent to the targeted sequence at the locus of interest... and 3.7 shows that we have only scratched the surface of modifications that can be made to Cas9. It is...

  18. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...quite large (500-600 bp) and may take up precious space in viral transfer vectors with limited packaging...stream of the IRES or 2A element (depending on the placement of the reporter gene). Addgene's collection offers...flanking the multicistronic element(s), or you could replace one of the reporter genes above with your gene ... are designed to have one or more of the genes replaced.  Additionally, 2A peptides could be PCR-cloned...

  19. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ..., the real value here is seeing them all in one place. Can we address problems in scientific publishing...of creating a technology that could eventually replace scientists themselves.  The sections on optogenetics...COVID times,” but the pandemic has certainly exacerbated them and forced scientists to change their priorities...introduction they need to grasp the challenges you face in your career.  Acknowledgements: I would like...

  20. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...is then washed, and either eluted from a fixed surface or precipitated, dried, and then rehydrated. There...glass particles in glass milk, gel slices are first placed in a chaotropic salt sodium iodine (NaI) solution...with ddH2O and then ethanol. After regeneration, trace amounts of DNA (3 femtograms/ul) could still be ...cleaned with 1M phosphoric acid and re-used, but trace contamination could interfere with interpreting ...

  21. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...gene therapy had fallen - plummeted really -  from grace 2 years prior with the death of Jesse Gelsinger ...these key elements together at the right time and place. But it is the hard work and persistence of a dedicated...early as next year. Twenty years after falling from grace, gene therapy is back, better and safer. It is now...therapy for blindness 2. Gene Therapy emerges from disgrace to be the next big thing, again 3. Acland G. et...

  22. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...yellow derivatives) A206K Interferes with dimer interface (monomeric derivatives) K26R, Q80R, N146H, H231L...biosensors has been designed to detect a variety of intracellular conditions, including ion (such as Ca2+) concentrations...available. Others: We’ve really just scratched the surface of the potential applications for GFP. It has also...strand break repair, and to label pathogenic intracellular microbes to visualize host/pathogen interactions...

  23. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...progress. You will also probably have a lot more face time with your bench mentor than your scientific...lot of details, especially when you are in a new place, learning something new. So how can you remember... walking through the protocol, by going to each space in order to double-check you remember where the ...Resources Resources on the Addgene blog Trading Spaces – Visiting Scientist Edition Mentoring for Scientists...

  24. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...target is present immediately upstream of a Protospacer Adjacent Motif (PAM), the sequence NGG.  If these... within the editing window (bases 4-8 of the protospacer). While this is a small window, this system can...system.  Fig. 1: Cytidine deamination takes place on the free strand of DNA and converts a C to U ...

  25. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ...express my concerns and request a replacement. I got my replacement in the end and the issue was resolved...less suitable for troubleshooting or issuing replacement products. In order to find good and helpful answers... in you – a technical support role is the right place to live it out, but ideally without asking too many...

  26. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...recombination; SSA, single strand annealing) took place and reconstituted the EGFP expression cassette. ...fragment containing the sgRNA target sequence can be placed in multi-cloning site (MCS) between EGFP fragments...extra precautions needed when dealing with RNA (e.g. face mask, and a dust free and specially cleaned work...mice that may have no mutation, costing money and space. As a rule, screen all of your pups unless you have...

  27. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...), the inner membrane space of mitochondria and chloroplasts, the extracellular milieu, and gram negative...should be taken into account when deciding where to place an FP within a fusion construct [1,2], i.e. positioning...mitochondria, lysosomes or they are secreted into the extracellular milieu. Many of the resident proteins within...

  28. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...is by no means exhaustive, but should be a good place to start when trying to pick your perfect promoter...promoter down regulation due to methylation or deacetylation. Ubc General expression mRNA Mammalian promoter...calmodulin. GAL1, 10 General expression mRNA Yeast adjacent, divergently transcribed promoters Inducible with...orientation to your gene Although this list is a great place to start, the tables above do not delve into the...

  29. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...The source of those conflicts can frequently be traced back to one of a few trouble spots:  Communication...here is pretty intuitive: You need to be willing to face conflict head-on. That doesn’t mean you have to ...and compromising. Though there may be a time and place for avoiding, competing, or accommodating, if you...knowledge to resolve conflicts. Conflict in the workplace is inevitable, but having who can help resolve...

  30. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...consuming and difficult. Finding volunteers who had space to shoot was another hurdle. As we slowly started...reopened. But there were still many restrictions in place, most importantly, masks.  Besides the muffled voices...strict safety protocols to keep Addgenies safe. Space was at a premium, so I had to figure out a solution... we were finally refining the process down to a place I could be happy with it. Cycle 5 - The Future! ...

  31. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...double-strand break (DSB). DSBs can be caused by intracellular factors such as nucleases and reactive oxygen...for DNA repair synthesis. The ssDNA strand then displaces one strand of the homologous DNA donor and can...formation of a DNA structure referred to as the displacement loop (D loop). The recombination intermediates...synthesized portion of the invasive strand is displaced from the template and returned to the processed...

  32. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...precision editing of the dystrophin gene would take place, with minimal risk of off-target editing. In order...sites. Each paper characterizes additional, unique facets of CRISPR DMD therapy. Long et al. show that AAV-CRISPR...the desired phenotype. Precision genome editing faces the challenges above and more. One chief challenge...approval. Even with the challenges precision editing faces, it’s encouraging to see such progress in the more...

  33. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...finding yourself having issues, you’re in the right place! In this post, we’ll talk about western blot optimization... visible to the naked eye.   If you see a smiley face in your gel, or if you see smeared bands, your voltage...voltage is likely too high. If you’re seeing a smiley face at an appropriate voltage (usually 10–15 V/cm of...bleed!), cut off the upper left-hand corner, or place a double ladder on the lefthand side. Whatever you...dry the membrane, wet it with 20% methanol, and place over a light box to visualize proteins after transfer...

  34. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ... site and adjacent PAM. Your knock-in design may naturally remove the gRNA sequence or place it far from...100 bp homology on each side. For optimal repair, place the loxP sites precisely at the cut site for each...the oviductal lumen, tweezer-type electrodes are placed to mediate electroporation. Ohtsuka et al. compared...

  35. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...members of your field, and present your research. Face to face meetings can make favorable impressions on potential...Medical Institute. Today's postdoctoral fellows (PFs) face a number of challenges ranging from long periods...manuscripts. 4. Balance focus and exploration Researchers face a fundamental conflict. Science is about exploration...

  36. Which Fluorescence Microscopy Techniques is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...reaching the detector. The pinhole is specifically placed to only allow light from the focal plane to reach...coverslip at an angle such that when it reaches the interface between the glass coverslip and a sample in aqueous...within a few hundred nanometers of the glass/water interface. Super resolution microscopy Super Resolution ...sample thin (< 15 μm) or thick? These questions will place each sample into one of four categories that are...

  37. AAV Packaged on Request is Here!

    Type
    Blog Post
    Published
    Jan. 10, 2025, 1:01 p.m.
    ...plasmids and your viral packaging service in the same place, making it easier to go from selection to viral ...to order at your convenience. After an order is placed, we’ll facilitate the necessary MTA, then start...your plasmid of interest in your desired serotype, place your order when ready, and we’ll take care of the...

  38. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    Published
    Sept. 25, 2018, 2:39 p.m.
    ...Can you imagine how much space a million plasmids would fill? Two weeks ago, Addgene edged passed our... hard to picture one million plasmids. How much space would one million bacterial stabs take up? If 81...upcoming months, we will be moving into a bigger space so that we can make an even bigger impact - we are...

  39. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ...depends on a surface glycoprotein – the only viral protein present on the virion surface – making it critical... system in a novel way, by shielding host cell surface proteins. Researchers at the University of Virginia...

  40. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ...factors – to specific sequences of DNA has become commonplace. This ability has opened up a world of possibilities...on a version that she hopes will change the intracellular localization of RNA. “You can think of changing...tips: First, she recommends using standard BsaI in place of the high fidelity version (despite what the original...

  41. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    Published
    May 1, 2014, 8:23 p.m.
    ...transfers. When Addgene entered the plasmid sharing space, we heard over and over again that the legal documents...experiments with confidence. Benefits of the UBMTA: Peace of mind Addgene has adopted the terms of the Uniform... Recipients: Streamlined Processing: When you place an order, Addgene generates the MTA and emails it...

  42. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    Published
    Dec. 5, 2019, 1:49 p.m.
    ...relatively the same for quite a while and hadn’t kept pace with our growth over the last few years. Some of... professional, and modern. Instead of trying to place all of the content above the fold, we decluttered...elements and allowed our content to dictate the space. We wanted to comfortably tell our story, to get...

  43. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    Published
    Feb. 14, 2019, 2:35 p.m.
    ...your workplace and you could end up making a huge impact on how waste is handled. A lot of places have...on waste, but also on culture. Encourage your workplace to become a leader in sustainability. Sign up...

  44. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    Published
    Nov. 22, 2016, 3:30 p.m.
    ... Many thanks to our guest blogger Sean Stacey! Sean D. Stacey is a Ph.D. student at East Tennessee State...This post was contributed by guest blogger Sean Stacey who recently attended both online and on site courses...

  45. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    Published
    March 15, 2018, 12:59 p.m.
    ... a double-strand break, and when used with two adjacent gRNAs, can lower the probability of off-target... of the targeted region, whereas PAM-in designs place the PAMs closer together in the middle of the targeted...sites separated by 51-68 bp Use D10A for HDR Place intended insert in between nick sites Use 40 nt...

  46. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    Published
    Sept. 12, 2023, 1:15 p.m.
    ... free online molecular biology tools all in one place. Plasmid mapping These tools are for viewing, editing... looking at plasmid maps and viewing sequencing traces, while the paid version provides more tools for...Removes non-DNA characters, such as numbers and blank spaces, from the text of a DNA sequence, making it ready...

  47. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    Published
    April 25, 2024, 1:15 p.m.
    ...perspectives. Such activities foster a mindset that embraces the unexpected and encourages exploration of tangents...ideas and approaches. Moreover, the ability to embrace serendipity fosters adaptability, a crucial skill...your academic journey, remember to leave a little space for the unexpected — it might just be the key to...

  48. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ...protein engineer currently working at Sanofi Pharmaceuticals in Cambridge, MA. When I joined Sanofi/Genzyme...continue to help bring HOPE to AJ.   PS: The superhero race is the perfect event for supporting AJ, who is a...protein engineer currently working at Sanofi Pharmaceuticals in Cambridge, MA. His work experience is in...

  49. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ... amazing job shuttling unwanted lab supplies to places that really need them.  When I was in graduate ...indefinitely at room temperature (thus also saving freezer space).  The procedure becomes less attractive when noting...very thin gel on a large glass slide using only surface tension.  It's a bit tricky to pour the right amount...

  50. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ..."CRISPR", short-hand for Clustered Regularly Interspaced Short Palindromic Repeats, in Pubmed returned...want to use double nickase, you could express two spacers and use PX335 to express the Cas9n (nickase). The...usually adds it when they order the oligo. If your spacer sequence starts with a 'G', you naturally have ...
Showing: 151 - 200 of 602 results