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We narrowed to 444 results for: Ank

Showing: 151 - 200 of 444 results

  1. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...resistance (Figure 2). This MazF-ZeoR cassette is flanked by two direct repeats which can be used for marker...through a second recombination event between the two flanking repeats. In cells where recombination has not ...

  2. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...or virology without acquiring materials from this bank. I am often asked, “What is the difference between...their identity. For example, perhaps a working cell bank has been made by a research institution (an excellent...

  3. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...transfer plasmid encodes your insert of interest, flanked by long terminal repeat (LTR) sequences, which ...Mansour, M. R., Schwarzwaelder, K., Bartholomae, C., Hubank, M., Kempski, H., Brugman, M. H., Pike-Overzet,...

  4. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...the addition of gene-specific 1 kb homology arms flanking a FLOXed neoR gene. pAAV-SEPT-Acceptor, the preferred... to deposit any new AAV tools you create. Many thanks to our guest blogger, Todd Waldman!  Todd Waldman...

  5. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...staff. Many thanks to our guest blogger, Stephanie Hays. Stephanie would like to additionaly thank Isaac Plant...

  6. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ... there is at least ~200 base pairs of sequence flanking the editing site on either side of the amplicon...screening by PCR may be the way to go. Design a PCR to flank your desired edit site and run the PCR product on...

  7. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...Also shown are the “B” restriction sites, which flank the targeted region. Upon PCR amplification, the...minor modifications to a plasmid template. Many thanks to our Guest Blogger Kristian Laursen! Kristian...

  8. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...Technique" in the comments section below! Many thanks to our guest blogger Krissy Lyon! Krissy Lyon is...32. PubMed PMID: 6176866. 3. Hoebeeck, Jasmien, Frank Speleman, and Jo Vandesompele. "Real-time quantitative...

  9. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...Duchenne muscular dystrophy. gRNAs can be designed to flank a single or multiple exons that, when deleted through...low to no off-target activity at the ten highest-ranking predicted off-target sites. Each paper characterizes...

  10. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...: Key elements (promoter, tag, polyA site) are flanked by unique restriction sites so you can swap sequences...endonuclease-reverse transcriptase uses homology arms flanking an RNA payload to insert the payload sequence ...

  11. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...Hippocampus labeled with anti-Kv2.1 (magenta), anti-AnkyrinG (green), and Hoechst nuclear stain (cyan). Panels...Immunofluorescence Staining. In W. H. Yong (Ed.), Biobanking (Vol. 1897, pp. 299–311). Springer New York. ...

  12. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...cost-effective and efficient manner than ever before, thanks to the development of site-specific nucleases (...the pitfalls common to other approaches. Many thanks to our Guest Bloggers Amar Singh and Mathew Goodwin...

  13. Finding Your Science Policy Path

    Type
    Blog Post
    Published
    Sept. 22, 2020, 1:15 p.m.
    ...networking---and they’re more accessible than ever thanks to the virtual world that the pandemic has foisted.... Image used with permission from SCI. Many thanks to our guest blogger Steph Guerra, a AAAS Science...

  14. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ...calculated p values from smallest to largest and ranking them (1 being the smallest value). It calculates...significance by finding the largest p value where its rank divided by the total number of comparisons performed...

  15. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...usually the products of teams: Watson, Crick, & Franklin, Meselson-Stahl, Hershey-Chase...Who was I to ...Discovery is exactly that: a myth. The original Dr. Frankenstein from Mary Shelley’s novel worked alone, but ...

  17. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...and Joung 2016. Many thanks to our guest blogger Søren Hough. We additionally thank Monica Sentmanat, Victor...

  18. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...overall. Type I systems degrade large sections of DNA thanks to the dual action power of Cas3. Cas3 functions...D. B. T., Gootenberg, J. S., Abudayyeh, O. O., Franklin, B., Kellner, M. J., Joung, J., & Zhang, F. (2017...

  21. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...essential to test the expression of your opsin. Thankfully the Deisseroth constructs coexpress a fluorescent...your optogenetics experiments. Stay tuned!  Many thanks to our guest blogger Derek Simon! Derek P. Simon...

  22. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    .... J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P.-F., Chen, C., Nelson, J. W., Newby...s41551-024-01227-1 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson...

  25. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ... with customizable N- and C-terminal modules   Thanks to the lab of Tomás Santalucía, you can now take...because they are transported back to the nucleus (thanks to a NLS on these proteins). The RFP proteins, ...Biol. 2015. PubMed PMID: 26000751   Thank you for reading. You can find all of our Hot Plasmid...

  26. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...Rivest, A.J., Jung, H.-Y., Kitamura, T., Suh, J., Frank, D., Kajikawa, K., Mise, N., Obata, Y., Wickersham..., W., Zhou, S., Beyer, J., Forsayeth, J., and Bankiewicz, K.S. (2013). Axonal transport of adeno-associated...

  27. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...from Cynthia Arokiaraj. Addgene would also like to thank Viviana Gradinaru and Tim Shay for their support...Stevenson, O., Vergara, J., Sharma, J., Zhang, Q., Franken, T. P., Smith, J., Ibrahim, L. A., Mastro, K. J...

  28. New Antibodies in the Lab Animation!

    Type
    Blog Post
    Published
    Nov. 19, 2024, 2:15 p.m.
    ...refresher on — these common antibody applications.  Thanks for watching, and we hope you found the video radd-ically...

  29. Meet Aavery, Blugene's New Virus Friend!

    Type
    Blog Post
    Published
    Feb. 13, 2020, 2:34 p.m.
    ...Blugene's new virus friend finally has a name! We thank you for your participation in suggesting names and...

  33. Addgene's 20th Anniversary Party

    Type
    Blog Post
    Published
    June 11, 2024, 1:15 p.m.
    ...recognizing our incredible depositors and requestors. Thank you all so much for an amazing twenty years of sharing...

  35. CRISPR 101 eBook...en español

    Type
    Blog Post
    Published
    Jan. 18, 2024, 2:15 p.m.
    ...helpful resources for their CRISPR experiments.  Many thanks to:  Daniel Mejías Sánchez, PhD, translator  Denise...

  37. Congrats to the 2019 Deck The Lab Winners

    Type
    Blog Post
    Published
    Jan. 17, 2020, 3 p.m.
    ...topper, tube “lights,” and autoclave tape garland. Thanks @ribosholmes!   Frogs and tacos The Miller Lab...

  38. Twenty Years of Sharing Science!

    Type
    Blog Post
    Published
    Jan. 8, 2024, 3:25 p.m.
    ... on our blog and social media. In the meantime, thank you all so much for being part of our community ...

  39. 8 Great Depositor Research Stories

    Type
    Blog Post
    Published
    Dec. 15, 2022, 2:15 p.m.
    ...available for other scientists to use. As always, a big thank you to our depositors for sharing your science with...

  40. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    Published
    May 17, 2017, 3:37 p.m.
    ...Our Segment on The Yeast Prototrophy Kit Special thanks to our sound engineer/producer, Eddy Page! Edward...

  41. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    Published
    Jan. 15, 2019, 2:19 p.m.
    ... Dyer Lab (@DyerLab_Emory) December 14, 2018 Thanks to all who participated in the 2018 #DeckTheLab...

  42. New Viral Vectors - Spring 2025

    Type
    Blog Post
    Published
    April 29, 2025, 1:30 p.m.
    ...However, our in-stock preps remain a popular choice thanks to the convenience of smaller sizes and quick shipping...

  43. Newly Updated AAV Data Hub!

    Type
    Blog Post
    Published
    Feb. 7, 2023, 2:15 p.m.
    ...you get interrupted by a freezer alarm going off, thanks to our handy-dandy "save" button. You can check...

  46. Fueled by Coffee at #SfN14

    Type
    Blog Post
    Published
    Nov. 18, 2014, 7:15 p.m.
    ...conservative estimate. As a graduate student, I drank 3-4 cups of coffee a day, and I had colleagues who...

  47. Addgene's Website Update

    Type
    Blog Post
    Published
    April 8, 2024, 2:15 p.m.
    ...will find Addgene’s website easier to navigate, thanks to design choices informed by Web Content Accessibility...

  48. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    Published
    Dec. 30, 2015, 3:30 p.m.
    ...accelerating research in labs all around the world. We thank our many depositors for making these reagents available...
Showing: 151 - 200 of 444 results