Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 317 results for: pho

Showing: 151 - 200 of 317 results

  1. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...example of how this works. Make sure to order 5’ phosphorylated oligos or treat with T4 PNK. Delivery options...

  3. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...inactivation maximum of 590 nm light. Aurora and Phobos optogenetic tools - A collection of bi-stable anion... medicine, evolution of body plans, synthetic morphology, and even artificial intelligence," says Michael...

  4. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...and cells. They can be used either with or as fluorophores in a wide variety of colors.  To antibody or... the Addgene blog Plasmids 101: Aptamer FluorophoresAntibodies 101: scFVs Antibodies 101: Introduction...

  5. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...selecting a bright fluorescent protein and a photostable fluorescent protein. In the third post of this...verify a number of key properties (brightness, photostability, oligomerization) of a number of promising ...

  6. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...costs low. Most will work with you remotely (by phone, Skype or Google Hangout) but in-person coaching...White Consulting frequently do sessions by Skype or phone. Samantha Sutton is another very experienced and...

  7. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...actions of the whole complex.  For example, the phosphorylation status of SRC-3 defines its association with...

  11. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ... of mCherry instead of BFP. Beyond switching fluorophores, why is FLEx useful? The power of the FLEx system...for clearer characterization of the location, morphology, and circuit mapping of a targeted neuronal population...

  13. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...responses of cryptochrome proteins or organic fluorophores (Karki et al., 2021; Lee et al., 2011; Matsuoka..., & Zoltowski, B. D. (2021). Cryptochromes: Photochemical and structural insight into magnetoreception...

  14. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...Screening Rinehart Lab Iterative Synthetically Phosphorylated Isomers (iSPI) Libraries ~11,000–110,139 ...

  15. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...commonly used promoters include the phosphate and copper inducible PHO5 and CUP1 promoters respectively. ...

  16. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    ...mismatch repair assay? Or is PCR followed by gel electrophoresis and/or next-generation sequencing more appropriate...   Lipid mediated Cationic polymers Calcium phosphate Cells Transformed cell lines Transformed cell...

  18. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ... This process can be avoided direct antibody fluorophore conjugates are used, but these are usually more...secondary nanobodies conjugated with the desired fluorophores. In figure 4B below, triple colocalization is...

  19. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...your samples and maintaining cellular and tissue morphology. Formaldehyde is the most commonly used cross-linker...reagents - cross-linkers better preserve sample morphology, while organic solvents can reveal otherwise ...

  20. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...basic science research (neuronal tracing and morphologic studies), as well as translational applications...capsids are useful tools for neuronal tracing and morphology studies when used as part of a two part fluorescent...

  23. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...including a lot of future Addgenies as shown in the photo below.  Kids and adults alike enjoyed some friendly...of your plans by email, gong, evite, posters, megaphone, or your company’s intranet. Don’t forget about...

  26. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...γ-Retrovirus Guide Protocols Agarose Gel Electrophoresis Bacterial Transformation How to Design a Primer... How to Design Primers for PCR Agarose Gel Electrophoresis Inoculating Liquid Bacterial Cultures Restriction...

  27. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...-I3, while initially dimmer, showed greater photostability and also matured considerably faster, with ...the intestine. Figure 3: Expression of fluorophore-tagged GOLG-4 through eight stages of embryonic...

  28. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...sample lysates will also contain proteases and phosphatases that may degrade the target protein. To prevent...prevent this, include protease and phosphatase inhibitors in the lysis buffer, keep samples on ice, and ...

  29. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...protein. VSV-G is a trimeric protein that binds phosphatidylserine and low-density lipoprotein receptors (LDLRs...in sera and augmented transduction of primary lymphocytes and CD34+ cells derived from human and nonhuman...

  30. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...development, extended-open integrins enable tissue morphogenesis, organ development, and the shaping of the extracellular...affinity reagents to study the difference in cell morphology changes induced by integrins binding the RGD ...

  31. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ... at Harvard University. Just as computers, cell phones, and cars become more technologically advanced ...sequences among DNA fragments separated by gel electrophoresis." Journal of molecular biology98.3 (1975): ...

  32. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...expression constructs fused with epitopes or fluorophores, generate expression vectors with various promoters...kinase is to express them with an appropriate phosphatase, a technique originally pioneered by Markus Seeliger...(Seeliger et. al. 2005). In this kit, Lambda phosphatase (Plasmid 79748) enhances bacterial expression...

  33. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    .... I've found cooking, gardening, hiking, and photography to be very satisfying. Finally, many PFs are...distance runner, gardener, cook, and outdoor photographer.  Additional Resources on the Addgene Blog ...

  35. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...(a thymine in this case) is only bound by the phosphate backbone. These PAM bases must be present for .... Instead, both lobes bind to the DNA and RNA phosphate backbones, allowing Cas9 to verify the structure...

  36. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...horseradish peroxidase protein (HRP) and alkaline phosphates. HRPs are usually preferred because of their ...conjugating your antibodies yourself. Alkaline phosphates have a linear reaction rate and are compatible...

  37. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ... by adding a protease inhibitor cocktail and phosphatase inhibitors to your lysis buffer. Additionally...recommendations. Tris-buffered saline (TBS) or phosphate-buffered saline (PBS) are the most commonly used...

  38. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    Published
    Jan. 31, 2018, 2:44 p.m.
    ... creative scientists delivered some spectacular photos once again this year! All winners receive an Addgene...

  41. Deck the Lab 2022!

    Type
    Blog Post
    Published
    Jan. 17, 2023, 2:15 p.m.
    ... contest, you need only decorate your lab, take photos, and then share them on Twitter or Instagram with...

  42. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    Published
    May 17, 2017, 3:37 p.m.
    ... down and read through each one, plug in some headphones to learn more about the latest and greatest plasmids...

  44. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    Published
    Sept. 25, 2018, 2:39 p.m.
    ...Here's the Addgene Leadership team in all their photo booth glory! #1millionplasmids On to the next million...

  45. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    Published
    Dec. 18, 2018, 1:17 p.m.
    ...Streptococcus pyogenes Cas9 inhibitor, with the LOV2 photosensor from Avena sativa, light-mediated genome editing...

  49. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ...light-responsive molecules of cryptochrome 2 are the chromophores flavin-adenine dinucleotide and methyltetrahydrofolate...

  50. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    Published
    Dec. 31, 2019, 2:26 p.m.
    ...Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins by Cary Valley, Shreya Vedantam, Michelle...
Showing: 151 - 200 of 317 results