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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 276 results for: ATC

Showing: 61 - 80 of 276 results

  1. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ...checked. Assuming full complementarity, its sequence matches the guide sequence. PAM: The “protospacer-adjacent... and target in order to cut DNA, whereas some mismatches can be tolerated outside this region. Conclusion...

  2. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    Published
    Jan. 24, 2019, 2:24 p.m.
    ...in your research.     To see Tetbow in action, watch the video below depicting dendrite wiring of mitral... and Takeshi Imai. "Optical clearing and index matching of tissue samples for High-resolution fluorescence...

  4. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...Plasmid Cloning Protocols Catch Up On Your Basic Molecular Biology Techniques Watch Our Protocol Videos ...

  6. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    Published
    Sept. 19, 2017, 1:20 p.m.
    ...developing embryos are transparent, so it’s easy to watch development in real time.  In both cases, dL5** ...light activation. Video: https://www.youtube.com/watch?v=xOtH5bs4HiI Advantages of MG-2I-dL5** over other...

  7. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...polymerase) is the ability to remove, reset and ligate mismatched overhangs, yielding scarless DNA assembly, and...the reaction mixture. The exonuclease removes mismatched 3’ overhangs, so that the region of homology ...

  8. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ... pathways. Individual cellular fates were then matched with candidate TFs responsible for that differentiation...collection, we’re releasing individual plasmids in batches. If there’s something you’d like that’s not yet...

  9. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ...efficiently collected by the microscope. To compare the match between a fluorescent protein and a filter set, ... emission spectra are red. Neither is a perfect match to the filter set but the excitation filter excites...

  10. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...Which Cas9 Do I Choose for My CRISPR Experiment A Match Made in Heaven: CRISPR/Cas9 and AAV Resources on...Resources Find CRISPR Plasmids for Your Research Catch up on Your CRISPR Background with Our Guide Pages...

  11. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...Did you catch our April AAV webinar with Tim Miles, PhD, Director of the CLOVER Center at CalTech? If...packages of 10…)  If you missed the webinar, you can watch the presentation and Q&A session on our YouTube ...

  12. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    Published
    Aug. 19, 2025, 1:15 p.m.
    ... neuronal activity, scientists can use GECIs to watch neurons fire under the microscope. To add another... usefulness depends on selecting a fluorophore matched to their excitation wavelength. OCaMP is well-suited...

  13. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ... If that’s not enough, we consult with experts, watch webinars, or use other research methods. In the...repositories. Then we format, do a final review to catch those pesky typos, and hit publish. Making content...

  14. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...proprietary. These methods also rely on ssDNA ends to match up regions of homology, so they won’t work if the...L-arabinose. These cells can be made and frozen in large batches suitable for many experiments. When REPLACR-mutagenesis...

  15. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    Published
    Feb. 18, 2014, 4:22 p.m.
    ...enough senior people willing to step up and be matched with the many interested mentees. So I experimented...in preparation for the meeting or to be read or watched during the meeting.  Scientists do better with ...

  17. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...prevalent issue with polyclonal antibodies is their batch to batch variability. Once an animal has undergone a...

  18. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...quite variable from antibody to antibody or batch to batch. For example, take lysine conjugation, which...

  19. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ... important step in ensuring quality, as it will catch any errors made during the cloning, sample handling... sequences to visually check for homology and mismatches. Do you have your own best sequencing practices...

  20. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...Plasmid Name Use a descriptive plasmid name and match the name as it appears in its publication or as ...spreadsheet, save it in the format: labname_addgene_batch_upload.xls Replace ‘labname’ with the name of...
Showing: 61 - 80 of 276 results