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Showing: 61 - 80 of 270 results

  1. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...Plasmid Cloning Protocols Catch Up On Your Basic Molecular Biology Techniques Watch Our Protocol Videos ...

  4. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...polymerase) is the ability to remove, reset and ligate mismatched overhangs, yielding scarless DNA assembly, and...the reaction mixture. The exonuclease removes mismatched 3’ overhangs, so that the region of homology ...

  5. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ... pathways. Individual cellular fates were then matched with candidate TFs responsible for that differentiation...collection, we’re releasing individual plasmids in batches. If there’s something you’d like that’s not yet...

  6. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    Published
    Sept. 19, 2017, 1:20 p.m.
    ...developing embryos are transparent, so it’s easy to watch development in real time.  In both cases, dL5** ...light activation. Video: https://www.youtube.com/watch?v=xOtH5bs4HiI Advantages of MG-2I-dL5** over other...

  7. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ...efficiently collected by the microscope. To compare the match between a fluorescent protein and a filter set, ... emission spectra are red. Neither is a perfect match to the filter set but the excitation filter excites...

  8. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...Which Cas9 Do I Choose for My CRISPR Experiment A Match Made in Heaven: CRISPR/Cas9 and AAV Resources on...Resources Find CRISPR Plasmids for Your Research Catch up on Your CRISPR Background with Our Guide Pages...

  9. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...Did you catch our April AAV webinar with Tim Miles, PhD, Director of the CLOVER Center at CalTech? If...packages of 10…)  If you missed the webinar, you can watch the presentation and Q&A session on our YouTube ...

  10. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ... If that’s not enough, we consult with experts, watch webinars, or use other research methods. In the...repositories. Then we format, do a final review to catch those pesky typos, and hit publish. Making content...

  11. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...proprietary. These methods also rely on ssDNA ends to match up regions of homology, so they won’t work if the...L-arabinose. These cells can be made and frozen in large batches suitable for many experiments. When REPLACR-mutagenesis...

  12. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    Published
    Feb. 18, 2014, 4:22 p.m.
    ...enough senior people willing to step up and be matched with the many interested mentees. So I experimented...in preparation for the meeting or to be read or watched during the meeting.  Scientists do better with ...

  14. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...prevalent issue with polyclonal antibodies is their batch to batch variability. Once an animal has undergone a...

  15. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...quite variable from antibody to antibody or batch to batch. For example, take lysine conjugation, which...

  16. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ... important step in ensuring quality, as it will catch any errors made during the cloning, sample handling... sequences to visually check for homology and mismatches. Do you have your own best sequencing practices...

  17. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...Plasmid Name Use a descriptive plasmid name and match the name as it appears in its publication or as ...spreadsheet, save it in the format: labname_addgene_batch_upload.xls Replace ‘labname’ with the name of...

  18. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...standard conditions be used when comparing different batches of virus. In addition, some viral preps are very... stored in the freezer. If you plan to freeze a batch of virus for long term use, you may want to consider...

  19. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...additionally remove all jewellery as well as that watch you’re wearing before you start. Also, avoid unnecessary...cell culture contamination in this post from the ATCC Declutter! Keep the hood clean Hoods should not ...

  20. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...very low concentration. So how do we make sure to catch all of these potential mishaps before distributing...suspect is contaminated. For AAV-68544, the best match for the contaminant is Addgene plasmid #44362. Method...
Showing: 61 - 80 of 270 results