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We narrowed to 148 results for: Coli

Showing: 61 - 80 of 148 results

  1. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...opposite, a uracil DNA N-glycosylase (UNG) from E. coli. The resulting construct, eUNG-BE4max(R33A)ΔUGI,... of 30 target sites. But the same strategy in E. coli produced different results. GBEs seemed to follow... by excising the freshly edited U bases — but E. coli instead filled those abasic sites with A's, producing.... In addition, they showed that UNG from both E. coli and C. elegans could be effective components of ...

  3. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ... With MAGIC, scientists introduce an Escherichia coli donor strain into the microbiome to deliver a genetic.... The lab used DCDC to measure growth rate of E. coli for over 10 generations and showed that this method...species such as Snodgrassella alvi and Gilliamella apicoli. The plasmids could also be transformed into Alphaproteobacteira...

  5. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...plasmid multimerization in the naturally occurring E. coli plasmid ColE1 (Goebel & Helinski, 1968; Bazaral ...Bedbrook & Ausubel, 1976; Fishel et al., 1981). E. coli strains such as DH5α, HB101, NEB Stable, Stbl3, ...harboring large inserts are often dimers in Escherichia coli K-12. Plasmid. 1989 Mar;21(2):138-41. doi: 10.1016...Characterization of multiple circular DNA forms of colicinogenic factor E-1 from Proteus mirabilis. Biochemistry...

  7. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    Published
    June 13, 2016, 7:08 p.m.
    ...addgene.org. If you are looking for plasmids used in E. coli and yeast, you’re in luck! Addgene has so many of...

  8. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...were used to express the necessary components in E. coli (Klompe et al., 2019). Recently, the lab introduced...temperatures. Data adapted from Vo et al., 2020. In E. coli, the new streamlined VchINT system was shown to ...

  9. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...with codon bias across all endogenous genes in E. coli and S. cerevisiae (Tuller et. al., 2010). ...codon optimizing a human gene for expression in E. coli. Genes are also optimized for expression in mammalian...

  10. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...from the bacterial genome, and inserted it into E. coli to express and then test for any proteins that could...addition, when they looked at the transformed E. coli expressing Cas7-11, they found no evidence of growth...

  11. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...described by studies of EcoRI and Bsp6I in Escherichia coli in 1995 when introduction of restriction-modification...Engelberg-Kulka, and Gad Glaser. "An Escherichia coli chromosomal" addiction module" regulated by guanosine...

  12. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...supplemental add-on kit and is compatible with E. coli and/or cell-free systems. Protein expression in ...al. “EcoFlex: A Multifunctional MoClo Kit for E. Coli Synthetic Biology.” ACS Synthetic Biology, vol. ...MoClo: Improved MoClo Assembly Standard and New E. Coli Part Library Enable Rapid Combinatorial Design for...

  17. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...simplified the process of generating multiple E. coli gene expression plasmids with the Cross-disciplinary... cloning that results in the creation of many E. coli constructs with greatly varied levels of expression...endeavours of unparalleled complexity in Escherichia coli, like the construction of a so-called “genomically... as a dominant-negative mutator allele of the E. coli MMR protein MutL, all under the control of the cI857...MutL is highly conserved in distant relatives of E. coli so it can be used in a broad range of strains. Thus...but these domains often do not express well in E. coli without their partner regulatory domains. The Chodera...

  18. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ... E. coli tRNA adenosine deaminases (ecTadA), fused it with dCas9, and transformed it into E. coli harboring...

  19. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...et al. "Expanding the genetic code of Escherichia coli with phosphoserine." Science 333.6046 (2011): 1151...flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation." Nature...
Showing: 61 - 80 of 148 results