We narrowed to 381 results for: ENA

...considered when using this method:     Cells must be amenable to transfection or viral transduction     Appropriate...studies in experimental systems that may not be amenable to plasmid based methods. One of the most common... "Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro...

...systems. Importantly, they allow transient delivery, enabling transgene-free genome editing (no transgene integration... used to simultaneously target multiple genes, enabling efficient multiplexed genome editing in a single... diverse plant and animal systems, potentially enabling more precise and accessible genome engineering...

...that overcome some current prime limitations and enable impressive editing efficiencies in vitro and in...simplifying the production process and could even enable the creation of CAR-T cells directly within the...Engineered allostery in light-regulated LOV-Turbo enables precise spatiotemporal control of proximity labeling...

...implemented and from that, develop better software.  Maintenance  We’re not just about developing new features...: we take time during each sprint to do some maintenance work on our code. One way to look at this is ...continuing to add new features.  Another area of maintenance is agreeing to the names of the things in our...

...standard, denaturing Western blot will not be suitable for an IP, as proteins in an IP are not denatured (learn...for an IP, samples are typically lysed in a non-denaturing buffer containing non-ionic detergents, such ...

... of glucose dehydrogenase and generated a strain that could achieve glucose dehydrogenase expression that... crRNA can be customized as well, including concatenations into crRNA arrays targeting multiple different...

...Principal Investigator meeting of the NSF EDGE (Enabling Discovery Through Genomic Tools) Program and I...the NSF program directors, this program should “enable the ability to study life’s innovations from anywhere...has created an automated tank for feeding and maintenance that had the audience gasping. Animal husbandry...

... provide a modern and fully supported API that enables development of custom integrations with other tools.... As illustrated in this video the integration enables RSpace users to search for protocols in protocols.io.... These integrations utilize an interface that enables users to conveniently select and deposit individual...

...generous donation of a Illumina MiSeq sequencer enabled us to perform the necessary sequencing on-site,...how the various pieces were connected, as this enabled us to rapidly iterate and optimize our pipeline...used Nextflow Tower to augment our pipeline and enable our team members to automate their weekly sequencing...

...can denature while aptamers are more stable and can go through cycles of denaturation/renaturation. ...

...eukaryotic genome to be entirely sequenced and is very amenable to genetic manipulation. Yeast cells are great...molecular biology processes. C. elegans are also quite amenable to genetic manipulation. Transgenic animals can...gene transfer in C. elegans: extrachromosomal maintenance and integration of transforming sequences." The...

...plant-specific changes to CRISPR components are necessary to enable genome editing in plant cells.   This blog post... empty gRNA backbones also contain Cas9, which enables expression of both Cas9 and the gRNA off of the... nanomaterials with chemical cargoes in plants enabled by a biorecognition motif. Nat Commun 11: . https...

...CRISPR has revolutionized molecular biology and has enabled functional studies in unprecedented ways. With ...This method is facilitated by DNA constructs that enable insertion of ~400 bp complementary to your gene...between targets) or multiple target sequences are concatenated. hpRNA has been extensively used in plant research...

...non-mammalian systems, but it is not confined to that arena! The Mammalian Toolkit was engineered specifically... Assembly Standard and New E. Coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional...McGee, Abby V., et al. Modular Vector Assembly Enables Rapid Assessment of Emerging CRISPR Technologies...

...Scores of FP variants have been created, thereby enabling a broad spectrum of donor fluorescence wavelengths...importantly, these compounds cross cell membranes, thus enabling FRET measurements in intact cells. The Gradia ...similarly but with differing R0 values, thereby enabling calibrated FRET distance measurements (for an ...

...keratinocytes from hair pluck, peripheral blood cells, and renal epithelial cells from urine are some of the easily...β-like-cells with the expression of pancreatic and duodenal homoeobox gene1 (PDX1) [40]. In recent works, ...3690668. 40. Ferber, S., et al., Pancreatic and duodenal homeobox gene 1 induces expression of insulin ...

...a thorough yet concise rundown of how recently enacted labor laws will likely increase postdoc earnings...graduate school classmates, Ben Vincent and Niroshi Senaratne. When we recorded, Ben and Niroshi, were finishing...

...senior graduate students, Ben Vincent and Niroshi Senaratne, from the Biological and Biomedical Sciences program...Biology, and he tweets @benjvincent.     Niroshi Senaratne is a graduate of the PhD program in Genetics and...

...about how FRET can be used to study biological phenomena and about how fluorophores other than fluorescent...Expression in Mammals Recombinase-based State Machines Enable Order-dependent Logic in vivo As always, feel ...

...series, we continue our conversation with Niroshi Senaratne and Ben Vincent from the Biological and Biomedical...Biology, and he tweets @benjvincent.     Niroshi Senaratne is a graduate of the PhD program in Genetics and...