...fetal microcephaly and Guillain-Barré syndrome. In response, the World Health Organization has declared a ... performing sensors and constructs encoding corresponding portions of the Zika virus genome with Addgene... American-lineages). Strain discrimination is especially important in the current outbreak because of ...the diagnostics into the hands of global health responders as soon as possible. Our longer-term aim is to...

...III), each responsible for transcribing different types of RNA: Polymerase I is responsible for ribosomal...ribosomal RNA, Polymerase II is responsible for mRNA and miRNAs, and Polymerase III transcribes tRNA and other...polymerase-associated proteins, CPSF and CstF, which are responsible for recruiting the cleavage and polyadenylation...

...time moaning about their troubles, Silent or unresponsive clams, who seem to ignore you and your questions...fellows are hesitant about expressing their ideas, especially if their supervisors are difficult people. The...will help you to communicate with confidence earn respect from your supervisor. Whenever possible, discuss... you even when you meet in person (silent or unresponsive clam), you will probably need support from your...

... inactivate specific genes in mammalian cells. Despite the popularity of CRISPR-based knockout/knock-in...-in systems, these systems remain valuable, especially in cases where CRISPR cannot be used (e.g. there... example shown in Figure 1, large sequences corresponding to exons 1 and 3 of the targeted gene have been...physiopathological events to which the chosen promoter responds. You can also use this method to tag an endogenous...

...product is toxic to cells at high concentrations. Despite their advantages, mammalian expressions systems...provides the highest level of protein expression. Despite their ease of use, it is important to note that... access and purification of a desired protein. Despite being a rather nascent technology, plant cells ...and copper inducible PHO5 and CUP1 promoters respectively. Yeast cells are grown in well-defined media...

...signaler. The pro of this system is the kinetic response time from signal input to readable output, which... is particularly powerful for in vivo imaging especially since JFs can cross the blood brain barrier and...molecule is added exogenously to measure a phenotypic response in the form of fluorescence or voltage indicators... to direct your voltage sensor selectively is especially essential. If you want to learn more about how...

...daily tasks, meet cross-team project commitments, respond to any issues raised by team members, and plan ... all the things for which you are officially responsible on paper, and take some time to write down all... have to exercise in order to manage these responsibilities. If you are in charge of completing many ...management and organizational skills. If you are responsible for working with lots of students, managing inventories...

...SARS-CoV-2 toward destabilizing one’s natural immune response to Adenovirus (and/or to its partner, AAV) or ...transient due to the fact that AdV surface proteins (especially fiber’s knob domain, and hexon), as well as other...all recruit strong innate and adaptive immune responses that inactivate viral genomes or otherwise clear...Sherman, L., & Verma, I. (1998). Cellular immune response to adenoviral vector infected cells does not require...

...result can leave scientists under-informed with respect to how different serotypes, promoters, and injection...conducted to improve our Addgene Data Hub, 76% of respondents indicated they wanted to see more negative data...flaws or gaps in their technical ability. But, especially for AAV data, that may not be the case. We can...transfer to the rat CNS: AAV PHP.EB vector dose–response and comparison to AAV PHP.B. Gene Therapy, 25(...

... Although we are busy with many other work responsibilities, we make team building activities a priority...don’t make things too complicated for yourself (especially if you are the sole event planner)! If you have...wise lady for many reasons, but this saying is especially true. If your event requires a lot of set up ...at Addgene. In addition to her operational responsibilities, she enjoys organizing team building events...

...LC) is known to play a major role in the stress response circuit by integrating neuronal signalling and...activated, norepinephrine release causes a freezing response, a form of immobility caused by stress and fear...surface. The latter reflects a state of behavioral despair, and the more time the animal spends in the passive...subset of excitatory neurons (Drd1) in mPFC is responsible for the antidepressant effect. To specifically...

...wheat, mushroom, and rice (Khatodia et al., 2016). Despite the almost universal functionality of the CRISPR...used to drive expression of small RNAs in their respective species. Addgene carries >30 “empty gRNA” backbones...activate or repress target genes in plant cells, respectively. Cas9 expression is commonly driven by plant-derived...excellent alternative genome editing technologies especially in human cells. In contrast to homology directed...

...synthesized enzyme. Biotin protein ligase (BPL) is responsible for this highly specific post-translational protein...biotinylated. In E.coli for example, the BPL, BirA, is responsible for biotinylating only one lysine in the entire...Esmond E. Snell was able to purify the protein responsible for binding biotin, avidin. Avidin was aptly ...your protein of interest or primary antibody respectively. Avidin bound to a reporter is then added to...

...differences in physiology, intelligence, and human correspondence that give them an edge as a disease mode (Iannaccone...also easier to study and at times more closely corresponds with human diseases and conditions. For example...scientific history, highly characterized genome, and widespread utilization, Arabidopsis has made significant...Arabidopsis genome project marked the beginning of widespread Arabidopsis genetics research development. Arabidopsis...

..... Ownership: Misunderstandings about who is responsible for what tasks or projects is a common source...customer support element amongst their other responsibilities. Knowing which aspects of customer support... require a manager to explicitly assign the responsibility in question to a specific person or team. ...one of your strengths. Empathizing with people, especially people with whom you strongly disagree, is a ...

...species on a single sample, you can then use the corresponding secondary antibodies conjugated to distinct ...), and will share some of their results and corresponding protocols. Other researchers may also have tested...Reviewing available antibodies can seem overwhelming, especially when there are dozens of products that all claim... find an antibody in which you are confident, especially if you work in an organism that is underrepresented...

...very rapidly in response to a single light pulse whereas others have slower responses (Gunaydin et al....different opsin (natural and engineered proteins responsive to light in combination with retinal) variations... along a pathway, there can be very different responses to long vs. short stimulation times or frequencies...

...to their Biosafety Level-2 or 2+. Please Note: Despite the fact that AAVs themselves are not pathogenic...expression (up to 9 months in mice [2]). Low Immune Response: Compared to other viral vectors (e.g. Lentivirus...Adenovirus) AAVs cause the least amount of immune response. Any investigator who is using an in vivo animal...model should consider the possible elicited immune response from viral infection. Animal safety is a top priority...

...sequence, a part of the env gene containing the rev response element (RRE), an internal promoter, and the desired...generation of replication competent lentiviruses (RCL) especially if you are working on HIV positive human cell...generating RCL is low but extra care has to be taken especially if you are working with proto-oncogenes or with...the Tet-on system. In the Tet-off system a Tet-response element (TRE) is placed upstream of the promoter...

... function. When a select group of people are responsible for a group of specific processes, they must ...tasks done. At Addgene, the Scientist Team is responsible for guiding new deposits into the repository,... not someone (or someones) taking on these responsibilities on a team to which you belong, consider stepping...roles they’re meant to fill and what their responsibilities are. That’s a subtle but important distinction...