...major players in the CRISPR field. Just a year after Feng Zhang’s lab identified Cas13a (C2c2) (Abudayyeh...opposite the target A to be edited. This change specifies the edit to be made when multiple As are present... see if they could shrink the construct. They identified ADAR2DD(delta984-1090), which decreases the REPAIRv1...the authors performed rational mutagenesis and identified that a S375A mutation in RESCUE (named RESCUE-S...

...It was by serendipity that I got into the field of gene therapy, more specifically AAV-based retinal ...trial gone horribly wrong. It brought the whole field to a grinding stop and only now, 2 decades later... work and a little bit of luck A pioneer in the field, Dr. Jean Bennett started working on ways to deliver...Gene therapy was in its infancy, but the eye was identified early on as an ideal target. It is an easily ...have broad tropism in the eye. AAV-2, the first identified serotype and the one used in Luxturna™, can efficiently...

...studied during graduate school. As a stable, easily purified protein with easily measured activity, MDH is ...Provost’s CURE prepare to analyze their recently purified MDH to study the impact of phosphorylation on ... genuine interest to other researchers in their field. Indeed, sharing results with the broader scientific... original and recombinant enzymes in bacteria, purified them to near homogeneity using a 6XHis tag, and...B. 2020. Mentorship and protégé success in STEM fields. Proceedings of the National Academy of Sciences...

...has been a long and winding path for many in the field. The vast majority of grant professionals didn’t...and by finding our individual niches within the field of grants. What is a grant professional?  In the...grants has fostered the demand for experts in the field of grants. It is no surprise that the U.S. Bureau...less than 400 international Grant Professionals certified through the Grant Professionals Certification ...by the Grant Professionals Association, and a Certified Social Media Strategist through the National Institute...

...that MPG could be engineered to act directly on unmodified G bases, resulting in a G → Y (Y = T or C) base...thymine Since an enzyme that excises inosine can be modified to target guanosine instead, perhaps analogous... et al., 2024). In each case, a UNG enzyme was modified to recognize T and/or C substrates. The HuidaGene...protein engineering going on in the base editing field! From CRISPR-free approaches to mitochondrial base...more diverse. We can’t wait to see what tools the field will come up with next! References and Resources...

... Cas9, Cas9 modified 9/12 known off-targets with a 14% indel rate, while ABE7.10 modified only 4/12 off-targets...target base for editing within the editing window specified by the Cas protein These elements were the starting...of dCas9. The resulting editor, BE3, nicks the unmodified DNA strand so that it appears “newly synthesized...efficiency. To further improve specificity, they purified HF-BE3 protein for delivery in ribonucleoprotein... and that they are expressed well. The Liu lab modified the nuclear localization signals and codon usage...

...isolation of modified cells; and (5) is compatible with the introduction of unique molecular identifiers, which... In vitro CRISPR screens in immune cells have identified many putative regulators of immunity. However... of the pRDA_526 vector. Moreover, the screen identified putative novel regulators of CD8+ T cell responses...

...many science policy professionals that entered the field from a more traditional policy background, learning...groups, attend webinars, and learn more about the field.  Read the CCST Science Policy Career Guide to learn...   Where can you do science policy? Because the field of science policy is so diverse, you can do it in...once selected, you can pivot to a completely new field without issue. This may be more challenging if you...you are trying to apply for an outright job in a field where you have no specific scientific credentials...

... editing. Repair oligos do not need to be PAGE purified, although doing so increases knock-in efficiency...CRISPR in C. elegans The Meyer and Mello labs also identified several experimental set ups that could improve...choosing. Tools such as SapTrap Builder greatly simplifies design of these constructs, allowing novice researchers...Developments in mammalian and yeast cells using modified Cas9 proteins to regulate gene expression (CRISPRi...systems, allowing rapid progress in a wide range of fields.   Thank you to our guest blogger! Jordan Ward...

... cell lines used in scientific research are misidentified! Rigor and reproducibility of scientific data...and collections. Importantly, Cellosaurus also identifies problematic cell lines: ones either suspected...or easily contaminated, as well as commonly misidentified lines. Browsing this feature to know if your... care about. If you have cell lines without a certified origin (you know, that split you got from the ...

... process, the insert and vector are first PCR amplified. The forward primer used to amplify the vector...PCR, you should confirm that the products were amplified by running a small aliquot of the reaction on ...2. Li, Chaokun, et al. "FastCloning: a highly simplified, purification-free, sequence-and ligation-independent... Our Special Collections for Plasmids from Your Field ...

...technology to survive the diverse demands of the biotech field, let's look at how prokaryotes originally utilized...generate RNA, which guide a Cas nuclease to the specified exogenous genetic material next to the species-specific...re-identifying it (3). Surprisingly, few genes have been identified that neutralize CRISPR/Cas. Earlier this year...lab protocols, providing tips from experts in the field, and enabling access to multiple plasmids used for...

... region. The variable region acts as a unique identifier for individual cells. Each cell is labeled with.... The fixed region acts as a library-specific identifier and allows cells to be tagged with different ...during reprogramming. These library-specific identifiers act like a timestamp and help demultiplex the...this lineage tracing experiment, the Morris lab identified a novel gene, Mettl7a1, whose expression helped...

...small molecules since the very beginnings of the field. Those engineered sensors were designed to replace...1980s. “Synthetic dyes were the standard in the field, but there is one problem: how to get that into ... sensors Griesbeck’s recent contribution to the field is a series of fluorescence resonance energy transfer...protein, troponin C (TnC), from muscle cells. They identified a toadfish TnC variant which had a high-affinity...

...pAAV-CAG-GFP 59462  AAV2 pAAV-CAG-tdTomato (codon diversified) 114472  AAV5, AAV8 pAAV-hSyn-mCherry 50459...for tracking large populations of neurons in wide-field imaging (Dana et al., 2018). See our full biosensor... For an estimate on availability date or to be notified when a particular catalog item is available for... the vector name and serotype you’d like to be notified about. Controls Plasmid Serotype Name 37825...

...selectable marker, and target gene, for example, are amplified with overlapping regions of homology and inserted...for transformation into yeast that same day.  The field of synthetic biology has led to advances in biofuel... in these methodologies offer streamlined and simplified alternatives to traditional cloning. NEBuilder...and opens the door for new opportunities in the field.  Gibson Assembly® is a registered trademark of ...

...conformational changes. These tiny movements are then amplified tens or hundreds of thousands of times by propagating...membranes outside of their natural context or 3) be modified to extend at different pH levels. Domesticating...soluble when extended (see figure to the right), we identified R-body mutants that extend at both lower and ...response to pH changes. Beyond these applications, purified R bodies could also perform as actuators in microelectromechanical...

...emission spectra. The lab of Dimitri Deheyn recently identified a family of GFP proteins in the cephalochordate...that you need. The Golden GATEway cloning kit simplifies the cloning process for complex DNA constructs...tumor markers to enhance cancer cell capture. Fierer et al., PNAS 2014 Apr 1;111(13):E1176-81. Zakeri...optimization through engineering, Ed Boyden’s lab has identified a variety of new channelrhodopsins for use as...for these effects, drawing on the rapidly growing field of optogenetics. Using a rational protein engineering...on the Golden Gate cloning method, but has been modified specifically to improve plant transgenesis. TheGreenGate...

...focused on current questions and experiments in the field of synthetic biology. Founded as a nonprofit in...biology has grown into a recognized name in the field of STEM education worldwide. The BioBuilder curriculum... as a program focused on an emerging scientific field, BioBuilder embraces new opportunities for scientific...module allows students to explore how engineering fields rely on rapid prototyping to develop and test hypotheses...

... use of microscopy in open science. But the new field of open-source microscopy seeks to change this. ...commercially available consumer-grade components. In this field, often referred to as 'frugal science', existing...Microscopy Sharing Hardware vs. Sharing Software In the field of microscopy, where image processing algorithms...Nevertheless, there are still many open questions to be clarified, especially with regard to licensing models. Direct...